Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae

Li, Qiurong; Zhang, Qiang; Wang, Meng; Liu, Fuzhong; Zhao, Sumin; Ma, Jian; Luo, Nan; Li, Ning; Li, Yousheng; Xu, Guowang; Li, Jieshou

doi:10.1016/j.abb.2007.06.023

Cited by 82 publications

(60 citation statements)

References 39 publications

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“…Previous reports in the literature suggest that treatment of cells with DHA and EPA causes a partial displacement of caveolin-1 from the lipid rafts and decreases signaling from molecules such as interleukin-1 in human umbilical cord endothelial cells (24,25). Our observations confirmed the previous literature and showed that the phenomenon also occurs in MDA-MB-231 breast cancer cells (Fig.…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Altenburg

Siddiqui

2009

Molecular Cancer Research

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Section: Discussionsupporting

confidence: 82%

“…Incorporation of DHA and EPA into the plasma membrane leads to disruption of the lipid raft domains (23). One of the markers for lipid rafts, caveolin-1, is partially displaced on treatment with DHA and EPA (24,25). Similarly, n-3 PUFAs have been shown to displace other signaling proteins from lipid rafts (26).…”

Section: Introductionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Altenburg

Siddiqui

2009

Molecular Cancer Research

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“…One of the mechanisms by which EPA and DHA are believed to improve vascular tone are by increasing endothelial cell membrane fluidity, and in fact DHA appears to be the more effective of the two in increasing plasma membrane fluidity of vascular endothelial cells (162) . In addition, DHA treatment increases the total n-3 LCP content of caveolae (invaginations of the plasma membrane where eNOS is located when it is inactivated) in cultured endothelial cells, and displaces the protein caveolin-1, which is responsible for securing eNOS in the caveolae region, from the caveolae (163) . This suggests that DHA may influence endothelial function by incorporation into the cell membrane, including caveolae, leading to an increase in eNOS activity and an inhibition of intracellular signalling pathways that may activate the inflammatory response (164) .…”

Section: Mechanisms For Modulation Of Vascular Function and Blood Prementioning

confidence: 99%

Dietary saturated and unsaturated fats as determinants of blood pressure and vascular function

Hall

2009

Nutr. Res. Rev.

144

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The amount and type of dietary fat have long been associated with the risk of CVD. Arterial stiffness and endothelial dysfunction are important risk factors in the aetiology of CHD. A range of methods exists to assess vascular function that may be used in nutritional science, including clinic and ambulatory blood pressure monitoring, pulse wave analysis, pulse wave velocity, flowmediated dilatation and venous occlusion plethysmography. The present review focuses on the quantity and type of dietary fat and effects on blood pressure, arterial compliance and endothelial function. Concerning fat quantity, the amount of dietary fat consumed habitually appears to have little influence on vascular function independent of fatty acid composition, although single high-fat meals postprandially impair endothelial function compared with low-fat meals. The mechanism is related to increased circulating lipoproteins and NEFA which may induce proinflammatory pathways and increase oxidative stress. Regarding the type of fat, cross-sectional data suggest that saturated fat adversely affects vascular function whereas polyunsaturated fat (mainly linoleic acid (18 : 2n-6) and n-3 PUFA) are beneficial. EPA (20 : 5n-3) and DHA (22 : 6n-3) can reduce blood pressure, improve arterial compliance in type 2 diabetics and dyslipidaemics, and augment endothelium-dependent vasodilation. The mechanisms for this vascular protection, and the nature of the separate physiological effects induced by EPA and DHA, are priorities for future research. Since good-quality observational or interventional data on dietary fatty acid composition and vascular function are scarce, no further recommendations can be suggested in addition to current guidelines at the present time.

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“…When incorporated into the membrane, EPA and DHA can alter this organisation, thus modulating signalling in various types of cells (121) . In endothelial cells, both EPA and DHA were shown to alter the organisation of caveolae, a particular subset of lipid rafts, and displace endothelial NOS from caveolae, a necessary step in the activation of endothelial NOS (122,123) . This could potentially lead to an increase in NO release by the endothelium and explain the putative beneficial effects of EPA and DHA on vasodilation observed to date ( Table 2).…”

Section: Arterial Compliancementioning

confidence: 99%

“…3-series prostacyclin is synthesised from EPA by endothelial cells, which adds on to the anti-aggregatory effect of 2-series prostacyclin (124) . In addition, both EPA and DHA inhibit plateletactivating factor synthesis (183) and stimulate endothelial NOS activity (122,123) in endothelial cells. The decrease in platelet-activating factor levels, as well as the increase of NO, which has anti-aggregatory properties, may also contribute to the anti-thrombotic effects of fish oils.…”

Section: Effects Of Epa and Dha On Thrombosis And Haemostasismentioning

confidence: 99%

The differential effects of EPA and DHA on cardiovascular risk factors

2011

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Compelling evidence exists for the cardioprotective benefits resulting from consumption of fatty acids from fish oils, EPA (20 :5n-3) and DHA (22 :6n-3). EPA and DHA alter membrane fluidity, interact with transcription factors such as PPAR and sterol regulatory element binding protein, and are substrates for enzymes including cyclooxygenase, lipoxygenase and cytochrome P450. As a result, fish oils may improve cardiovascular health by altering lipid metabolism, inducing haemodynamic changes, decreasing arrhythmias, modulating platelet function, improving endothelial function and inhibiting inflammatory pathways. The independent effects of EPA and DHA are poorly understood. While both EPA and DHA decrease TAG levels, only DHA appears to increase HDL and LDL particle size. Evidence to date suggests that DHA is more efficient in decreasing blood pressure, heart rate and platelet aggregation compared to EPA. Fish oil consumption appears to improve arterial compliance and endothelial function; it is not yet clear as to whether differences exist between EPA and DHA in their vascular effects. In contrast, the beneficial effect of fish oils on inflammation and insulin sensitivity observed in vitro and in animal studies has not been confirmed in human subjects. Further investigation to clarify the relative effects of consuming EPA and DHA at a range of doses would enable elaboration of current understanding regarding cardioprotective effects of consuming oily fish and algal sources of long chain n-3 PUFA, and provide clearer evidence for the clinical therapeutic potential of consuming either EPA or DHA-rich oils.

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Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae

Cited by 82 publications

References 39 publications

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Dietary saturated and unsaturated fats as determinants of blood pressure and vascular function

The differential effects of EPA and DHA on cardiovascular risk factors

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