Do Tryptase, ECP and Specific IgE Measurement by Nasal Incubation Increase the Specific Nasal Provocation Test Sensitivity?

Bellussi, Luisa; Marcucci, Francesco; Sensi, L.; Passàli, Gc; Lauriello, M; Passali, Fm; Giannuzzi, A. L.; Passàli, Desiderio

doi:10.1177/039463200401700212

Cited by 12 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the cardiovascular system this receptor is present on both adherent cardiovascular system cells and on circulating inflammatory cells (39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53) . It is involved in balancing degradation processes initiated by inflammatory cells and in the response of adherent cells to alterations in surrounding extra cellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Expression of the High-Affinity Laminin Receptor (67 kDa) in Normal Human Skin and Appendages

Cavalieri

Rotoli

Feliciani

et al. 2005

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

The interaction of cells with extracellular matrix components plays a significant role in the regulation of cell biology. Laminin is a large glycoprotein involved in fundamental interactions between cells and the basement membrane. Several cell surface receptors are responsible for cell-matrix interactions. The 67 kDa high affinity laminin receptor, 67LR, is involved in the adhesion of normal cells to the laminin network and is also associated with the metastatic phenotype of some tumoral cells. We have investigated the expression of laminin and of the 67LR in normal human skin using immunoperoxidase staining. Twenty samples of skin were analyzed. Antibody against laminin reacted in a continuous linear band at the dermal-epidermal junction, as well as basement membranes of hair follicles, sebaceous and eccrine sweat glands, and dermal blood vessels. The epidermis and the follicular epithelium were negative for laminin. The 67LR seemed not to be expressed on the basal surface of basal keratinocytes. The major expression of this receptor may be detected in the upper half of the spinous layer and in the granular layer. The cells of the outer root sheath in hair follicle showed the same immunohistochemical pattern described for epidermis. In sebaceous glands and in eccrine sweat glands the secreting epithelium was positive. Endothelial cells of dermal blood vessels were routinely positive for 67LR. We observed that the expression of the 67LR in normal human skin is mostly located in epidermal areas in which the keratinizing process was particularly advanced.The interaction of cells with extracellular matrix components plays a significant role in the regulation of cell morphology, growth and differentiation and contributes to the pathogenesis of cancer (1). Extracellular matrix organization is particularly complex at the interface between histologically dissimilar tissues that originate from different primary germ layers. In this area the so-called basement membrane is formed (2).The skin basement membrane zone is among the most complex basement membranes of the human body (3). Several macromolecules have been identified within the basement membrane zone. Among these laminin is a ubiquitous, large glycoprotein with a molecular weight of approximately 900 kDa. It is composed of three genetically different subunits, named A, B I and B2 in the classical form, which assemble into a cruciform structure with one long arm and three short arms (4-5).Laminin is thought to form a major basement membrane network located in the lamina lucida (6) and to be involved in important interactions between cells and basement membrane (7). Epidermal cells and extracellular matrix proteins interact through specific receptors of the cellular surface. To date, severallaminin binding proteins have been identified (8). The high affinity laminin receptor, molecular weight 67 kDa, was the first laminin receptor to be identified in 1983 (9-11).This receptor is physiologically expressed by a large variety of cells and seems to be involved in the a...

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of the High-Affinity Laminin Receptor (67 kDa) in Normal Human Skin and Appendages

Cavalieri

Rotoli

Feliciani

et al. 2005

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

show abstract

“…It would be interesting to evaluate the possible correlation between response to anti-TNF alpha molecules and molecular or cellular mechanisms of inflammation (apostosis, oxidative stress, lymphocyte and mast cell functions and so on) (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

Infliximab Monotherapy for Refractory Psoriasis: Preliminary Results

Cassano

Loconsole

Аморусо

et al. 2004

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

Tumour necrosis factor (TNF)-alpha plays an important role in the pathogenesis of psoriasis. Infliximab is an anti-TNF-alpha chimeric monoclonal antibody, which is licensed for the treatment of rheumatoid arthritis and Crohn's disease. Some reports have shown the efficacy of infliximab, either in monotherapy or in combination with methotrexate, for the treatment of psoriatic arthropathy and psoriasis. The efficacy and tolerability of infliximab monotherapy was evaluated in 29 patients with moderate to severe psoriasis, unresponsive to conventional treatments. Fourteen patients suffered from concomitant arthropathy. Patients received intravenous infliximab, 5mg/kg, at weeks 0, 2, and 6. After this 3-dose-induction regimen, patients were followed-up at monthly intervals and retreated with a single-dose infusion in case of relapse of signs and symptoms. Clinical assessment was performed using the psoriasis area and severity index (PASI) to monitor psoriasis activity; pruritus and joint pain were assessed on a scale of 0 to 3. A marked improvement of skin lesions and subjective symptoms was noted in the majority of patients; an excellent reduction of PASI score (⩾75%) was observed in 13.8% of cases at week 2, 71.4% at week 6 and 78.6% at week 10. During the follow-up period, some patients maintained satisfactory clinical results without requiring any additional infusions. In general, skin lesions showed a trend towards a more prolonged and sustained improvement as compared with subjective symptoms. Treatment was well tolerated and no serious adverse events occurred.

show abstract

“…The measurement of cells and mediators in the nose may increase the sensitivity of nasal challenges (1303)(1304)(1305)(1306) but more data are needed.…”

Section: Nasal Challenge Testsmentioning

confidence: 99%