Do the intervertebral disc cells respond to different levels of hydrostatic pressure?

Hutton, William; Elmer, William A.; Bryce, Lisa M.; Kozłowska, E.; Boden, Scott D.; Kozlowski, Miroslav

doi:10.1016/s0268-0033(01)00080-8

Cited by 64 publications

(64 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of a constant hydrostatic pressure has been shown to affect disc cell metabolism in a magnitude-and durationdependent manner. A constant hydrostatic pressure of 0.3-1 MPa increased matrix synthesis in bovine caudal disc tissue, dog IVD cells in alginate and even human pathological disc tissue but hydrostatic pressure higher than 3 MPa was found to induce a catabolic response of the disc cells [36,42,43,46,65].…”

Section: In Vitro Organ Culturementioning

confidence: 94%

“…The influence of static hydrostatic pressure has been studied on isolated human and animal disc cells [42,43,65] (Table 3). The application of a constant hydrostatic pressure has been shown to affect disc cell metabolism in a magnitude-and durationdependent manner.…”

Section: In Vitro Organ Culturementioning

confidence: 99%

“…It has been shown in various in vivo and in vitro models that a low magnitude of dynamic compression [70,122] and static/dynamic hydrostatic pressure [31,36,43,46,50,65,86,98,124] at a physiological frequency could increase anabolic response, but a high magnitude of dynamic loading causes early signs of disc damage and catabolic remodelling (Tables 2, 3). …”

Section: Factors Affecting Ivd Responses To Loadingmentioning

confidence: 99%

See 2 more Smart Citations

The effects of dynamic loading on the intervertebral disc

2011

View full text Add to dashboard Cite

Loading is important to maintain the balance of matrix turnover in the intervertebral disc (IVD). Daily cyclic diurnal assists in the transport of large soluble factors across the IVD and its surrounding circulation and applies direct and indirect stimulus to disc cells. Acute mechanical injury and accumulated overloading, however, could induce disc degeneration. Recently, there is more information available on how cyclic loading, especially axial compression and hydrostatic pressure, affects IVD cell biology. This review summarises recent studies on the response of the IVD and stem cells to applied cyclic compression and hydrostatic pressure. These studies investigate the possible role of loading in the initiation and progression of disc degeneration as well as quantifying a physiological loading condition for the study of disc degeneration biological therapy. Subsequently, a possible physiological/beneficial loading range is proposed. This physiological/beneficial loading could provide insight into how to design loading regimes in specific system for the testing of various biological therapies such as cell therapy, chemical therapy or tissue engineering constructs to achieve a better final outcome. In addition, the parameter space of 'physiological' loading may also be an important factor for the differentiation of stem cells towards most ideally 'discogenic' cells for tissue engineering purpose.

show abstract

Section: In Vitro Organ Culturementioning

confidence: 94%

Section: In Vitro Organ Culturementioning

confidence: 99%

Section: Factors Affecting Ivd Responses To Loadingmentioning

confidence: 99%

See 1 more Smart Citation

The effects of dynamic loading on the intervertebral disc

2011

View full text Add to dashboard Cite

show abstract

“…For example, 4 h of loading is required to up-regulate aggrecan and collagen I1 levels in the annulus fibrosus, but our results do not indicate whether four 1 h loadings applied over four days would have the same effect. Additionally, while changes in matrix gene expression have been found to correlate well to synthesis levels and protein concentration [8,15,37], mechanical loading has been shown to increase the amount of active enzyme (MMP-2) without affecting mRNA or protein concentrations [13]. Since matrix remodeling is a synergistic relationship between anabolic and catabolic processes, the added effect of enzyme activation makes it difficult to predict the long-term effects of our loading conditions with gene expression alone, and highlights the need for additional long-term studies capable of detecting changes in protein composition.…”

Section: Discussionmentioning

confidence: 99%

The effects of short‐term load duration on anabolic and catabolic gene expression in the rat tail intervertebral disc

MacLean

Lee

Alini

et al. 2005

Journal Orthopaedic Research

125

120

View full text Add to dashboard Cite

The goal of this study was to determine the time-dependent response of the intervertebral disc cells to in vivo dynamic compression. Forty-seven skeletally mature Wistar rats (> 12 months old) were instrumented with an Ilizarov-type device spanning caudal disc 8-9. Using a load magnitude (1 MPa) and frequency (1.0 Hz) that were previously shown to significantly alter mRNA levels in the disc, the effects of 0.5 and 4 h of loading were investigated and compared to a sham group and our previous 2 h results. Annulus and nucleus tissue of loaded (c8-9) and internal control discs ( c 6 7 and c10-11) were separately analyzed by real-time RT-PCR for levels of mRNA coding for various anabolic (collagen-lAl, collagen-2A1, aggrecan) and catabolic (MMP-3, MMP-13, ADAMTs-4) proteins. In the annulus, mRNA levels increased for Collagen types I & 11, and MMP 3 & 13 with increasing load duration. In contrast, the nucleus had the largest increases in aggrecan, ADAMTs-4, MMP-3 and MMP-13 after 2 h of loading, with aggrecan and MMP-13 mRNA levels returning to control values after 4 h of loading. Taken in context with our previous studies, we conclude that intervertebral disc cells from the nucleus and annulus have distinct responses to dynamic mechanical compression in vivo with sensitivity to compression magnitude, frequency and duration.

show abstract

“…To elucidate the mechanism by which such degeneration occurs and progresses, several studies have been performed, including in vitro experiments on cultured disc cells [11][12][13] and tissues [17,18,41] under mechanical stresses. The murine tail disc model has been frequently adopted in animal model studies for producing disc degeneration-like changes by the application of artificial mechanical loading because it is inexpensive and convenient to perform.…”

Section: Introductionmentioning

confidence: 99%

Effects of compressive loading on biomechanical properties of disc and peripheral tissue in a rat tail model

et al. 2009

View full text Add to dashboard Cite

Intervertebral disc degeneration induced by mechanical compression is an important issue in spinal disorder research. In this study, the biomechanical aspect of the rat tail model was investigated. An external loading device equipped with super-elastic TiNi springs was developed to apply a precise load to the rat tail. By using this device, rat tail discs were subjected to compressive stress of 0.5 or 1.0 MPa for 2 weeks. Discs in the sham group received an attachment of the device but no loading. After the experimental period, first the intact tail with peripheral tissues (PT) such as tendon and skin and then the retrieved disc without PT were subjected to a uniaxial tension-compression test; biomechanical characteristics such as range of motion (ROM), neutral zone (NZ), and hysteresis loss (HL) were evaluated. Furthermore, the loadbearing contribution of PT in the intact tail was estimated by comparing the load-displacement curves obtained by the mechanical tests performed with and without PT. The experimental findings revealed that the continuous compressive stress induced reduction in disc thickness. The intact tail demonstrated decreases in ROM and NZ as well as increases in HL. On the other hand, the retrieved disc demonstrated increases in ROM, NZ, and HL. Further, a significant increase in the load-bearing contribution of PT was indicated. These findings suggest that the load-bearing capacity of the disc was seriously deteriorated by the application of compressive stress of 0.5 or 1.0 MPa for 2 weeks.

show abstract

Do the intervertebral disc cells respond to different levels of hydrostatic pressure?

Cited by 64 publications

References 16 publications

The effects of dynamic loading on the intervertebral disc

The effects of dynamic loading on the intervertebral disc

The effects of short‐term load duration on anabolic and catabolic gene expression in the rat tail intervertebral disc

Effects of compressive loading on biomechanical properties of disc and peripheral tissue in a rat tail model

Contact Info

Product

Resources

About