Do Plant Caspases Exist?

Woltering, E.J.; Bent, A. van der; Hoeberichts, Frank A.

doi:10.1104/pp.006338

Cited by 156 publications

(124 citation statements)

References 30 publications

(35 reference statements)

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“…23,25,[36][37][38] This is the first study of caspase-like activity in a gymnosperm plant. Neither caspase-1-nor 3-like-activities previously reported for angiosperm cell death systems 8,9,11,13 were found to be involved in spruce embryonic cell death. It is possible that the observed differences in substrate specificity between angiosperm and gymnosperm caspase-like proteases are attributed to a long phylogenetic distance between the two groups of higher plants.…”

Section: Discussionmentioning

confidence: 56%

“…Although the implication of plant metacaspases in programmed cell death has not been shown so far, yeast metacaspase YCA1 has been demonstrated as an effective executor for cell death, displaying increased proteolytic activity against caspase-6-specific synthetic substrate VEID-7-amino-4-methylcoumarin (AMC). 7 Second, there is a growing body of physiological and biochemical data suggesting that plant cells contain caspase-1-(i.e., YVADase) and/or 3-like (i.e., DEVDase) proteolytic activities, 8 which correlate with the level of experimentally induced cell death, [9][10][11][12][13] and that the latter can be abolished or delayed by synthetic [9][10][11][12][13] or natural 14,15 caspase-specific inhibitors. As most of these experiments dealt with pathogenor drug-induced cell deaths, they cannot address the roles of caspase-related proteins (metacaspases) and caspase-like proteolytic activity in normal plant development.…”

Section: Introductionmentioning

confidence: 99%

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VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation

et al. 2003

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Plant embryogenesis is intimately associated with programmed cell death. The mechanisms of initiation and control of programmed cell death during plant embryo development are not known. Proteolytic activity associated with caspase-like proteins is paramount for control of programmed cell death in animals and yeasts. Caspase family of proteases has unique strong preference for cleavage of the target proteins next to asparagine residue. In this work, we have used synthetic peptide substrates containing caspase recognition sites and corresponding specific inhibitors to analyse the role of caspase-like activity in the regulation of programmed cell death during plant embryogenesis. We demonstrate that VEIDase is a principal caspase-like activity implicated in plant embryogenesis. This activity increases at the early stages of embryo development that coincide with massive cell death during shape remodeling. The VEIDase activity exhibits high sensitivity to pH, ionic strength and Zn 2 þ concentration. Altogether, biochemical assays show that VEIDase plant caspase-like activity resembles that of both mammalian caspase-6 and yeast metacaspase, YCA1. In vivo, VEIDase activity is localised specifically in the embryonic cells during both the commitment and in the beginning of the execution phase of programmed cell death. Inhibition of VEIDase prevents normal embryo development via blocking the embryo-suspensor differentiation. Our data indicate that the VEIDase activity is an integral part in the control of plant developmental cell death programme, and that this activity is essential for the embryo pattern formation.

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Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation

et al. 2003

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“…All these classes of proteases have been shown to be involved in programmed cell death (PCD) in mammalian systems, with the cysteinyl-aspartic-proteases (caspases) as key cell death executioners (Grütter, 2000). Caspaselike activity also executes PCD in plant cells (Coll et al, 2011;Woltering et al, 2002), and the active process operates through constitutively expressed machinery (Elbaz et al, 2002). Their potential involvement in HR has Author manuscript, published in Plant Physiology and Biochemistry (2014) 77: 84-89 DOI:10.1016/j.plaphy.2014 been reported following the infection of TMV in tobacco (Del Pozo and Lam, 2003).…”

Section: Discussionmentioning

confidence: 99%

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Benouaret

Goujon

Goupil

2014

Plant Physiology and Biochemistry

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To cite this version:Razik Benouaret, Eric Goujon, Pascale Goupil. Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells. Plant Physiology and Biochemistry, Elsevier, 2014Elsevier, , 77, pp.84-89. <10.1016Elsevier, /j.plaphy.2014.01.021>. Author manuscript, published in Plant Physiology and Biochemistry (2014) 77: 84-89 DOI:10.1016/j.plaphy.2014 1 -A polyphenol-rich extract from grapes induced chemoperception in tobacco suspension cells -GME induced modification of plasma membrane properties -GME elicits defence genes in tobacco cells -GME-induced cell death was mediated by proteases and de novo protein synthesis -This study evidences the ability of GME to induce hypersensitive response Graphical abstractHighlights Abstract: Grape marc extract (GME) showed elicitor activity on suspension-cultured cells of tobacco. The BY-2 cells reacted to GME (0.25% and 0.125%) with a long-sustained pH rise in their growth medium. Using EGTA or LaCl 3 , we showed that extracellular alkalinization depended on Ca 2+ mobilization. The tobacco BY-2 cells challenged with GME promoted cell death and the upregulation of defence-related genes such as PR3, PAL and CCoAOMT. Cell death rate was quantified using an experimental calibrated Evans Blue assay. The GMEinduced cell death was dose-dependent and occurred in 24 h. Longer exposure increased the extent of tobacco cell death. To investigate a potential hypersensitive reaction, we tested the effect of various inhibitors of protein synthesis (cycloheximide) and proteases (aprotinin, pepstatin and E-64) on GME-induced cell death. All these chemicals reduced GME-induced cell death rate in 30 min. Overall, our findings indicate that GME elicits early perception events, defence reactions and cell death requiring protein synthesis and proteases.

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“…It has been shown that there are certain morphological and biochemical similarities between plant and animal PCD. 4 Thus, it is useful to compare the PCD of plants and animals in developing strategies to study PCD in plants.…”

Section: Dear Editormentioning

confidence: 99%

Evidence for cytochrome f involvement in eggplant cell death induced by palmitoleic acid

Peters

Chin

2005

Cell Death Differ

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Do Plant Caspases Exist?

Cited by 156 publications

References 30 publications

VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation

VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Evidence for cytochrome f involvement in eggplant cell death induced by palmitoleic acid

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