For this study, 373 students of H cyber university were chosen to conduct a survey in the spring semester of 2011. The result of this study through structural equation modeling analysis was as follows: First, organizational support significantly affected perceived isolation. Second, organizational support and perceived isolation significantly affected satisfaction. Third, perceived isolation and satisfaction significantly affected learning persistence, while organizational support didn't. In addition, satisfaction was verified as a mediating variable between organizational support, satisfaction, and learning persistence, and satisfaction was verified as a mediating variable between perceived isolation, organizational support and learning persistence. These results imply that perceived isolation and organizational support should be considered for the design and development strategies of instructional courses in order to enhance satisfaction and learning persistence of students in cyber educational environment.