Do neurons in the vertebrate CNS migrate on laminin?

Liesi, Päivi

doi:10.1002/j.1460-2075.1985.tb03755.x

Cited by 239 publications

(139 citation statements)

References 52 publications

(53 reference statements)

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“…By contrast, the incorporation of laminin (or a suitable hydrogel e.g. fibrin [50]) into bridges increases axon regeneration and disrupts gliotic scar formation in vivo [51][52][53][54]. PDL has additional effects in promoting neuronal cell adhesion [55].…”

Section: Discussionmentioning

confidence: 99%

An in vitro spinal cord injury model to screen neuroregenerative materials

Weightman¹,

Pickard²,

Yang³

et al. 2014

Biomaterials

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Implantable 'structural bridges' based on nanofabricated polymer scaffolds have great promise to aid spinal cord regeneration. Their development (optimal formulations, surface functionalizations, biocompatibility, topographical influences and degradation profiles) is heavily reliant on live animal injury models. These have several disadvantages including invasive surgical procedures, ethical issues, high animal usage, technical complexity and expense. In vitro 3-D organotypic slice arrays could offer a novel solution to overcome these challenges, but their utility for nanomaterials testing is undetermined. We have developed an in vitro model of spinal cord injury that replicates stereotypical cellular responses to neurological injury in vivo, viz. reactive gliosis, microglial infiltration and limited nerve fibre outgrowth. We describe a facile method to safely incorporate aligned, poly-lactic acid nanofiber meshes (± poly-lysine + laminin coating) within injury sites using a lightweight

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Section: Discussionmentioning

confidence: 99%

An in vitro spinal cord injury model to screen neuroregenerative materials

Weightman¹,

Pickard²,

Yang³

et al. 2014

Biomaterials

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“…In most neuronal tissues, development of the axons and formation of new synapses is preceded by migration of the neuronal cell bodies to the appropriate regions of the brain. It has been proposed that LN facilitates all of these processes in several neural systems [51][52][53]. The spatio-temporally controlled expression of LN-1 in the developing peripheral nervous system, as well as the visual pathway and the cerebellum, suggests that LN plays a role in axon outgrowth and guidance [54][55][56].…”

Section: Discussionmentioning

confidence: 99%

Differences between the effect of anisotropic and isotropic laminin and nerve growth factor presenting scaffolds on nerve regeneration across long peripheral nerve gaps

2008

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Anisotropic scaffolds of agarose hydrogels containing gradients of laminin-1 (LN-1) and nerve growth factor (NGF) molecules were used to promote sciatic nerve regeneration across a challenging 20 mm nerve gap in rats.Step and continuous gradient anisotropic scaffolds were fabricated and characterized and regeneration was compared to that in isotropic scaffolds with uniform concentrations of LN-1 and NGF and sciatic nerve grafts harvested from syngenic rats. Polysulfone tubular guidance channels were used to present the agarose based scaffolds to the nerve stumps. Fourmonths after implantation, regenerating axons were observed in animals implanted with anisotropic scaffolds with gradients of both LN-1 and NGF molecules and nerve grafts, but not in animals with isotropic scaffold implants. Also, the scaffolds with gradient of either LN-1 or NGF, with the other component being uniformly distributed in the scaffold, did not elicit axonal regeneration. The total number of myelinated axons was similar for the anisotropic scaffold and the nerve graft conditions, with the anisotropic scaffolds having a higher density of axons than the nerve grafts. Axonal diameter distribution was similar for the anisotropic scaffolds and the nerve grafts. The nerve grafts and anisotropic scaffolds resulted in better functional outcome compared to isotropic scaffolds as measured by the relative gastrocnemius muscle weight (RGMW). Additionally the state of neuromuscular junctions as assessed by pre-and post-synaptic staining revealed that both the anistropic scaffolds performed as well as nerve grafts.

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“…Laminin-1 expression in the mouse brain is high before birth but is downregulated postnatally (Liesi, 1985) before dendrites undergo their most rapid growth. This observation suggests that other cues activate integrins to regulate dendrite development in the postnatal brain.…”

Section: Arg Is Required For Integrin-mediated Responses To Semaphorimentioning

confidence: 99%

Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases

Moresco¹,

Donaldson²,

Williamson³

et al. 2005

J. Neurosci.

134

133

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Dendrite arbor structure is a critical determinant of nervous system function that must be actively maintained throughout life, but the signaling pathways that regulate dendrite maintenance are essentially unknown. We report that the Abelson (Abl) and Abl-related gene (Arg) nonreceptor tyrosine kinases are required for maintenance of cortical dendrites in the mouse brain. arg Ϫ/Ϫ cortical dendrites initially develop normally and are indistinguishable from wild-type dendrites at postnatal day 21. Dendrite branches are not efficiently maintained in arg Ϫ/Ϫ neurons, leading to a reduction in dendrite arbor size by early adulthood. More severe dendrite loss is observed in abl Ϫ/Ϫ arg Ϫ/Ϫ neurons. Elevation of Arg kinase activity in primary cortical neurons promotes axon and dendrite branching. Activation of integrin receptors by adhesion to laminin-1 or Semaphorin 7A also promotes neurite branching in cortical neurons, but this response is absent in arg Ϫ/Ϫ neurons because of the reduced dynamic behavior of mutant neurite branches. These data suggest that integrin signaling through Abl and Arg support cortical dendrite branch maintenance by promoting dendrite branch dynamics in response to adhesive cues.

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Do neurons in the vertebrate CNS migrate on laminin?

Cited by 239 publications

References 52 publications

An in vitro spinal cord injury model to screen neuroregenerative materials

An in vitro spinal cord injury model to screen neuroregenerative materials

Differences between the effect of anisotropic and isotropic laminin and nerve growth factor presenting scaffolds on nerve regeneration across long peripheral nerve gaps

Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases

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