Do mRNAs act as direct sensors of small molecules to control their expression?

Stormo, Gary D.; Ji, Yongmei

doi:10.1073/pnas.181334498

Cited by 83 publications

(36 citation statements)

References 28 publications

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“…Significantly, the biosynthesis genes of these metabolites are encoded by those same mRNAs Winkler et al 2002). This confirmed earlier predictions that nature uses metabolitemRNA interactions for translational control of proteins involved in metabolite biosynthesis (Nou and Kadner 1998;Gelfand et al 1999;Stormo and Ji 2001). It is reasonable to expect that more examples of natural metabolite-mRNA translational switches will soon be discovered.…”

Section: Nature's Use Of Rna As a Metabolic Switchsupporting

confidence: 79%

Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA

Silverman¹

2003

RNA

110

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Switches and sensors play important roles in our everyday lives. The chemical properties of RNA make it amenable for use as a switch or sensor, both artificially and in nature. This review focuses on recent advances in artificial RNA switches and sensors. Researchers have been applying classical biochemical principles such as allostery in elegant ways that are influencing the development of biosensors and other applications. Particular attention is given here to allosteric ribozymes (aptazymes) that are regulated by small organic molecules, by proteins, or by oligonucleotides. Also discussed are ribozymes whose activities are controlled by various nonallosteric strategies.

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Section: Nature's Use Of Rna As a Metabolic Switchsupporting

confidence: 79%

Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA

Silverman¹

2003

RNA

110

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“…For example, thiamine (also known as vitamin B 1 ) controls the expression of the genes involved in thiamine biosynthesis via the thi box of the mRNA (129). Similar models have been proposed for cobalamin and vitamin B 12 , indicating that regulation can be conferred not exclusively by proteins but also by small molecules (142,221). The mRNA elements that directly monitor environmental conditions have been termed riboswitches and are involved in several metabolic pathways (for example, biosynthesis of vitamins and metabolism of methionine, lysine, and purines) (240).…”

Section: Regulation Of Translation Initiationmentioning

confidence: 79%

Initiation of Protein Synthesis in Bacteria

Laursen

Sørensen

Mortensen

et al. 2005

Microbiol Mol Biol Rev

530

506

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Valuable information on translation initiation is available from biochemical data and recently solved structures. We present a detailed description of current knowledge about the structure, function, and interactions of the individual components involved in bacterial translation initiation. The first section describes the ribosomal features relevant to the initiation process. Subsequent sections describe the structure, function, and interactions of the mRNA, the initiator tRNA, and the initiation factors IF1, IF2, and IF3. Finally, we provide an overview of mechanisms of regulation of the translation initiation event. Translation occurs on ribonucleoprotein complexes called ribosomes. The ribosome is composed of a large subunit and a small subunit that hold the activities of peptidyltransfer and decode the triplet code of the mRNA, respectively. Translation initiation is promoted by IF1, IF2, and IF3, which mediate base pairing of the initiator tRNA anticodon to the mRNA initiation codon located in the ribosomal P-site. The mechanism of translation initiation differs for canonical and leaderless mRNAs, since the latter is dependent on the relative level of the initiation factors. Regulation of translation occurs primarily in the initiation phase. Secondary structures at the mRNA ribosomal binding site (RBS) inhibit translation initiation. The accessibility of the RBS is regulated by temperature and binding of small metabolites, proteins, or antisense RNAs. The future challenge is to obtain atomic-resolution structures of complete initiation complexes in order to understand the mechanism of translation initiation in molecular detail

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“…Several groups have reported that the conserved cores of TPP riboswitches (Miranda-Rios et al 2001;Stormo and Ji 2001;Rodionov et al 2002) and FMN riboswitches (Gelfand et al 1999;Vitreschak et al 2002) Nahvi, J.E. Barrick, and R.R.…”

Section: Resultsmentioning

confidence: 99%

Metabolite-binding RNA domains are present in the genes of eukaryotes

Sudarsan

Barrick

Breaker

2003

RNA

392

320

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Genetic control by metabolite-binding mRNAs is widespread in prokaryotes. These riboswitches are typically located in noncoding regions of mRNA, where they selectively bind their target compound and subsequently modulate gene expression. We have identified mRNA elements in fungi and in plants that match the consensus sequence and structure of thiamine pyrophosphate-binding domains of prokaryotes. In Arabidopsis, the consensus motif resides in the 3-UTR of a thiamine biosynthetic gene, and the isolated RNA domain binds the corresponding coenzyme in vitro. These results suggest that metabolite-binding mRNAs are possibly involved in eukaryotic gene regulation and that some riboswitches might be representatives of an ancient form of genetic control.

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Do mRNAs act as direct sensors of small molecules to control their expression?

Cited by 83 publications

References 28 publications

Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA

Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA

Initiation of Protein Synthesis in Bacteria

Metabolite-binding RNA domains are present in the genes of eukaryotes

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