Background : Biohazard incidents are ordinary situations usually managed by health systems with the mandatory priority of preventing the spread of the pathogen. Health care workers in charge of dealing with these situations must be equipped with personal protective equipment (PPE) for her/his own security. The main objective of this study was developing a risk model to predict whether health care workers will tolerate wearing PPE, III category, 4B / 5B / 6B type, against biological risks during a 30 minutes intervention. Methods : A preliminary, prospective, simulation study, without intervention was conducted at the Advanced Simulation Center at the Medicine Faculty of Valladolid University (Spain) from April 3rd to 28th, 2017. Students and professional's health care were equipped with a PPE and performed a 30 minutes-long biohazard simulation. Anthropometric, physiological, analytical variables, and anxiety levels were measured pre- and post-simulation. A scoring model was constructed by using the estimate regression coefficients of the significant variables obtained from a multivariate model of the logistic regression for the outcome variable. Results : 96 volunteers with median age of 26 years (25th-75th percentile: 22-41 years) of which 56 (58.3%) were women enter into the test. Half of the sample presented metabolic fatigue after 20 minutes of finishing the simulation. The predictive model included female sex, height, both muscle and bone mass and moderate level of physical activity. The validity of the main model using all the variables presented an area under the curve (AUC) of 0.86 (95%CI: 0.786-0.935), and the validity of the model presented an AUC of 0.725 (95%CI: 0.559-0.89). Conclusions : Decision-making in biohazard incidents is a challenge for emergency team leaders. An a priori knowledge of physiological tolerance of wearing a PPE of the health care workers could improve their performance. The model presented here could help in the assessment of the worker response under biohazard conditions.