Do maggots have an influence on bacterial growth? A study on the susceptibility of strains of six different bacterial species to maggots of Lucilia sericata and their excretions/secretions

Cazander, G.; Veen, K.E.B. van; Bernards, A.T.; Jukema, G. N.

doi:10.1016/j.jtv.2009.02.005

Cited by 57 publications

(64 citation statements)

References 25 publications

(23 reference statements)

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“…From our results and those of other researchers, it is vital to select optimal conditions in which to detect antibacterial activity in L. sericata ES (Thomas et al, 1999;Bexfield et al, 2004;Jaklic et al, 2008;Cazander et al, 2009b). Whilst it is important to use media with a sufficiently high nutritional content for normal bacterial growth in the control vessel, it is also important that the amount and type of media employed is not detrimental to the antibacterial activity exhibited by the ES.…”

Section: Discussionmentioning

confidence: 74%

“…Consequently, the different concentrations of ES and types of media employed in the LCAs will have contributed to the variation in results to date reported by previous researchers (Thomas et al, 1999;Bexfield et al, 2004;Jaklic et al, 2008;Cazander et al, 2009b).…”

Section: Discussionmentioning

confidence: 96%

“…Whilst it is important to use media with a sufficiently high nutritional content for normal bacterial growth in the control vessel, it is also important that the amount and type of media employed is not detrimental to the antibacterial activity exhibited by the ES. Addition of nutrients such as 33% TSB, 50% brain heart infusion broth and 50% Mueller Hinton broth, where poor control is reported (Thomas et al, 1999;Jaklic et al, 2008;Cazander et al, 2009b) would have influenced the effectiveness of antibacterial activity in ES. This contrasts with inhibition of bacterial growth achieved over a 24-hour period in media low in nutrient such as in 10% peptone water (Bexfield et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…However, a review of studies provided contradictory results on the duration and potency of antibacterial activity against common wound bacterial species. For example, some research demonstrated L. sericata ES inhibited growth of Pseudomonas aeruginosa over a 24-hour period at an estimated concentration of 5g larvae per ml (Bexfield et al, 2004) whereas others reported a prolonged lag phase of 5-6 hours at an estimated concentration of 7.5 g larvae per ml (Jaklic et al, 2008) or either partial or no inhibition of P. aeruginosa (Thomas et al, 1999;Cazander et al, 2009b).…”

Section: Introductionmentioning

confidence: 99%

“…The pH of larval ES has in part been thought to influence its antibacterial effectiveness (Thomas et al, 1999;Cazander et al, 2009b;Gwatkin and Fallis, 1938). However, studies have since proved this not to be the case (Bexfield et al, 4 2004;Barnes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Barnes

Dixon

Gennard

2010

Journal of Microbiological Methods

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Research to quantify the potency of larval excretion/secretion from Lucilia sericata using liquid culture assays has produced contradictory results. In this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and the effects of varying growing conditions in assays. Results demonstrate that factors such as number of larvae, species of bacteria and addition of nutrient influence its antibacterial potency. Therefore a standardised method should be employed for liquid culture assays when investigating the antibacterial activity of larval excretion/secretion from L. sericata

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Barnes

Dixon

Gennard

2010

Journal of Microbiological Methods

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Maggot Therapy for Wound Debridement

Opletalová

Blaizot²,

Mourgeon³

et al. 2012

Arch Dermatol

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To study the efficacy of bagged larvae on wound debridement compared with conventional treatment. Design: Randomized, multicenter, controlled, prospective phase 3 trial with blinded assessment of outcome measures by a single observer. Setting: Two hospital referral centers in Caen and Lyon, France. Patients: Random sampling of 119 patients with a nonhealing, sloughy wound 40 cm 2 or smaller, less than 2 cm deep, and an ankle brachial index of 0.8 or higher. Intervention: During a 2-week hospital stay, patients received either maggot debridement therapy (MDT) or conventional treatment. At discharge, conventional dressings were applied and a follow-up visit occurred at day 30. Main Outcome Measure: Percentage of slough in wounds at day 15. Results: There was a significant difference between groups at day 8 (54.5% in the MDT group and 66.5% in the control group) (P = .04). The mean percentage of slough at day 15 was 55.4% in the MDT group and 53.8% in the control group (P=.78). Conclusions: Although MDT shows no significant benefit at day 15 compared with conventional treatment, debridement by MDT is significantly faster and occurs during the first week of treatment. Because there is no benefit in continuing the treatment after 1 week, another type of dressing should be used after 2 or 3 applications of MDT.

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Multiple actions of Lucilia sericata larvae in hard‐to‐heal wounds

et al. 2013

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In Europe ≈15,000 patients receive larval therapy for wound treatment annually. Over the past few years, clinical studies have demonstrated the success of larvae of Lucilia sericata as debridement agents. This is based on a combination of physical and biochemical actions. Laboratory investigations have advanced our understanding of the biochemical mechanisms underlying the beneficial effects of larval secretions, including removal of dead tissue, reduction of the bacterial burden, and promotion of tissue regeneration. The present article summarizes our current understanding of the microbiological, immunological, and wound healing actions of larval therapy, and the molecules involved in these beneficial effects. Future studies will focus on the isolation, identification, and (pre)clinical testing of the effective molecules of L. sericata larvae. These molecules may be candidates for the development of new agents for the treatment of several infectious and inflammatory diseases, including chronic wounds.

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Do maggots have an influence on bacterial growth? A study on the susceptibility of strains of six different bacterial species to maggots of Lucilia sericata and their excretions/secretions

Cited by 57 publications

References 25 publications

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): Variation in laboratory evaluation

Maggot Therapy for Wound Debridement

Multiple actions of Lucilia sericata larvae in hard‐to‐heal wounds

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