Do lamin B1 and lamin B2 have redundant functions?

Lee, John M.; Jung, Hea-Jin; Fong, Loren G.; Young, Stephen G.

doi:10.4161/nucl.29615

Cited by 12 publications

(7 citation statements)

References 30 publications

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“…This finding was supported by studies in which the Lmnb2 locus was replaced with Lmnb1, and vice versa. Lmnb1 at the Lmnb2 locus could not substitute for Lmnb2, and vice versa, resulting in brain phenotypes similar to those of Lmnb1 −/− and Lmnb2 −/− mice 115 . In addition, mice that express a nonfarnesylated form of Lmnb2 develop normally, as opposed to those that express nonfarnesylated Lmnb1, which die at birth with severe neurodevelopmental defects.…”

Section: Insights From Animal Modelsmentioning

confidence: 99%

“…In fact, mice with disruptions in Lmnb1 and/or Lmnb2 develop to term but die perinatally, with developmental defects in lung, bone, and brain 112–114 . Because Lmnb1 and Lmnb2 have 60% homology and similar expression patterns 115 , functional redundancy could account for the relatively mild phenotype. However, Lmnbl −/− and Lmnb2 −/− mice develop to term at the expected Mendelian frequency 113 .…”

Section: Insights From Animal Modelsmentioning

confidence: 99%

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Lamins and Lamin-Associated Proteins in Gastrointestinal Health and Disease

et al. 2018

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The nuclear lamina is a multi-protein lattice composed of A- and B-type lamins and their associated proteins. This protein lattice associates with heterochromatin and integral inner nuclear membrane proteins, providing links among the genome, nucleoskeleton, and cytoskeleton. In the 1990s, mutations in EMD and LMNA were linked to Emery-Dreifuss muscular dystrophy. Since then, the number of diseases attributed to nuclear lamina defects, including laminopathies and other disorders, has increased to include more than 20 distinct genetic syndromes. Studies of patients and mouse genetic models have pointed to important roles for lamins and their associated proteins in the function of gastrointestinal organs, including liver and pancreas. We review the interactions and functions of the lamina in relation to the nuclear envelope and genome, the ways in which its dysfunction is thought to contribute to human disease, and possible avenues for targeted therapies.

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Section: Insights From Animal Modelsmentioning

confidence: 99%

Section: Insights From Animal Modelsmentioning

confidence: 99%

Lamins and Lamin-Associated Proteins in Gastrointestinal Health and Disease

et al. 2018

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“…This might be due to functional redundancy with LamC or other lamina proteins. Comparable to mice mutant for the B-type Lamin [ 41 ], Drosophila flies homozygous for a null mutation in Lam are able to reach the adult stage [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Expression of lamina proteins Lamin and Kugelkern suppresses stem cell proliferation

Petrovsky

Großhans

2018

Nucleus

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The nuclear lamina is involved in numerous cellular functions, such as gene expression, nuclear organization, nuclear stability, and cell proliferation. The mechanism underlying the involvement of lamina is often not clear, especially in physiological or developmental contexts. Here we investigate the role and activity of farnesylated lamina proteins Lamin (Lam) and Kugelkern (Kuk) in proliferation control of intestinal stem cells (ISCs) in adult Drosophila flies. We found that ISCs mutant for Lam or kuk proliferate, whereas overexpression of Lam or Kuk strongly suppressed proliferation. The anti-proliferative activity is, at least in part, due to suppression of Jak/Stat but not Delta/Notch signaling. Lam expression suppresses Jak/Stat signaling by normalization of about 50% of the Stat target genes in ISCs.

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“…For example, Hovens et al demonstrated that in rats, IBA-1 could be used to evaluate microglial activation by analyzing cell body to cell size ratio (Hovens et al, 2014). Vasculature (vimentin and collagen IV) and nuclear (lamin B1) markers permit to study alterations of the anatomical organization of the tissue, for example after a stroke, or investigating chromatin organization (Bianchini et al, 2021) and neuronal migration during brain development (Lee et al, 2014). Finally, S6RP is a downstream effector of mTOR and its level of phosphorylation reflects the activation of the mTORC1 complex.…”

Section: Discussionmentioning

confidence: 99%

Exploring the human cerebral cortex using confocal microscopy

Pesce

Laurino

Scardigli

et al. 2021

Preprint

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Cover-all mapping of the distribution of neurons in the human brain would have a significant impact on the deep understanding of brain function. Therefore, complete knowledge of the structural organization of different human brain regions at the cellular level would allow understanding their role in the functions of specific neural networks. Recent advances in tissue clearing techniques have allowed important advances towards this goal. These methods use specific chemicals capable of dissolving lipids, making the tissue completely transparent by homogenizing the refractive index. However, labeling and clearing human brain samples is still challenging. Here, we present an approach to perform the cellular mapping of the human cerebral cortex coupling immunostaining with SWITCH/TDE clearing and confocal microscopy. A specific evaluation of the contributions of the autofluorescence signals generated from the tissue fixation is provided as well as an assessment of lipofuscin pigments interference. Our evaluation demonstrates the possibility of obtaining an efficient clearing and labeling process of parts of adult human brain slices, making it an excellent method for morphological classification and antibody validation of neuronal and non-neuronal markers.

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Do lamin B1 and lamin B2 have redundant functions?

Cited by 12 publications

References 30 publications

Lamins and Lamin-Associated Proteins in Gastrointestinal Health and Disease

Lamins and Lamin-Associated Proteins in Gastrointestinal Health and Disease

Expression of lamina proteins Lamin and Kugelkern suppresses stem cell proliferation

Exploring the human cerebral cortex using confocal microscopy

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