Do hyphae pulse as they grow?

Jackson, Sandra

doi:10.1046/j.0028-646x.2001.00228.x

Cited by 19 publications

(16 citation statements)

References 25 publications

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“…Tip growth rates in fungi and oomycetes can be variable but are usually between 1 and 10 mm min 21 (McKerracher & Heath, 1987;Jackson, 2001). This is comparable to the speed that the oil droplets were moving at in the hyphae and is consistent with a role for mass flow in moving the cytoplasm forward as the tip extends.…”

Section: Discussionmentioning

confidence: 60%

A pressure gradient facilitates mass flow in the oomycete Achlya bisexualis

Muralidhar

Swadel

Spiekerman³

et al. 2016

Microbiology

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We have used a single cell pressure probe and observed movement of microinjected oil droplets to investigate mass flow in the oomycete Achlya bisexualis. To facilitate these experiments, split Petri dishes that had media containing different sorbitol concentrations (and hence a different osmotic potential) on each side of the dish were inoculated with a single zoospore. An initial germ tube grew out from this and formed a mycelium that extended over both sides of the Petri dish. Hyphae growing on the 0 M sorbitol side of the dish had a mean turgor (¡SEM) of 0.53¡0.03 MPa (n513) and on the 0.3 M sorbitol side had a mean turgor (¡SEM) of 0.3¡0.027 MPa (n59). Oil droplets that had been microinjected into the hyphae moved towards the lower turgor area of the mycelia (i.e. retrograde movement when microinjected into hyphae on the 0 M sorbitol side of the split Petri dish and anterograde movement when microinjected into hyphae on the 0.3 M sorbitol side of the Petri dish). In contrast, the movement of small refractile vesicles occurred in both directions irrespective of the pressure gradient. Experiments with neutral red indicate that the dye is able to move through the mycelia from one side of a split Petri dish to the other, suggesting that there is no compartmentation. This study shows that hyphae that are part of the same mycelia can have different turgor pressures and that this pressure gradient can drive mass flow.

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Section: Discussionmentioning

confidence: 60%

A pressure gradient facilitates mass flow in the oomycete Achlya bisexualis

Muralidhar

Swadel

Spiekerman³

et al. 2016

Microbiology

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“…Horizontal helical growth would give constant elongation in the x-y plane because the tip would always be elongating at a constant angle to the long axis of the hypha. Only helical growth at some inclination to the horizontal could generate apparent pulses as envisaged by Jackson (2001). It is possible to model the apparent growth pulses from such an effect (Table 2).…”

Section: Possible Explanations Of Previous Datamentioning

confidence: 99%

“…One possible artefactual source of periodic pulsatile growth patterns has been described by Jackson (2001). She argues that periodic oscillations of growth direction in the z plane could be sufficient to create an illusion of pulsatile growth in many species, yet remain undetected within the plane of focus of the microscope.…”

Section: Possible Explanations Of Previous Datamentioning

confidence: 99%

“…It is possible to model the apparent growth pulses from such an effect (Table 2). Determination of the radius of the helix is uncertain, but Jackson (2001) has argued that if it is less than about 0?5 mm it might be undetectable. As seen in Table 2, amplitudes and periods comparable to those previously reported for hyphae can be generated from inclined helical growth, but only at inclinations of about 10u to the horizontal.…”

Section: Possible Explanations Of Previous Datamentioning

confidence: 99%

“…Money (1997) presented a very limited set of data for Achlya, showing fluctuating, but certainly not periodic, growth and subsequently showed fluctuating force exerted by hyphal tips of the same species (Johns et al, 1999). More recently, Jackson (2001) showed that potentially undetected growth oscillations in the vertical plane could create artefactual growth pulses. In spite of the limited data supporting pulsatile hyphal tip growth, it has been accepted as a universal feature of hyphae, indicated by the conclusion to a recent commentary: ''I suspect that pulsatile growth will be recognized as a universal feature of polarized cellular development'' (Money, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Time series analysis demonstrates the absence of pulsatile hyphal growth

2003

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Hyphal tip growth has been previously reported as pulsatile, defined as regularly alternating fast and slow rates of extension. The growth of pollen tubes, and hyphae of Neurospora crassa and Saprolegnia ferax were analysed using high spatial and temporal resolution. By using long (100-500 s) records of growth rate, sampled every second, it was possible to apply rigorous statistical analysis of the time series. As previously demonstrated, pollen tubes can show pulsatile growth, detectable with this system. In contrast, hyphal growth rates do not show any evidence of pulsatile growth; instead, growth rates appear to fluctuate randomly. It is concluded that pulsatile growth is not a common feature of hyphal tip growth.

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Branching in Fungal Hyphae and Fungal Tissues

Moore

McNulty

Meškauskas

Branching Morphogenesis

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Do hyphae pulse as they grow?

Cited by 19 publications

References 25 publications

A pressure gradient facilitates mass flow in the oomycete Achlya bisexualis

A pressure gradient facilitates mass flow in the oomycete Achlya bisexualis

Time series analysis demonstrates the absence of pulsatile hyphal growth

Branching in Fungal Hyphae and Fungal Tissues

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