Do graphene oxide nanostructured coatings mitigate bacterial adhesion?

Wuolo-Journey, Karl; BinAhmed, Sara; Linna, Elise; Castrillón, Santiago Romero-Vargas

doi:10.1039/c9en00499h

Cited by 11 publications

(7 citation statements)

References 69 publications

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“…Taken together, these results imply that the different degrees of GO exposure affect the antimicrobial activity of membranes. Previous studies have indicated that direct contact between GO nanosheets and bacterial cells is essential in designing antibacterial membranes with two-dimensional materials. ,,, As schematically illustrated in Figure , the coating strategy enhanced GO exposure for cell inactivation, while the GO nanosheets were embedded in the PDA layer using the blending strategy, which hindered direct contact between nanosheets and cells.…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Functionalized Membranes: The Importance of Nanosheet Surface Exposure for Biofouling Resistance

Cheng

Kaneda

et al. 2019

Environ. Sci. Technol.

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Surface functionalization using two-dimensional (2D) graphene oxide (GO) materials is a promising technique to enhance the biofouling resistance of membranes used in water purification and reuse. However, the role of GO exposure, which is crucial for the contact-mediated toxicity mechanism, has not been well evaluated or elucidated in previous studies. Herein, we employ bioinspired polydopamine chemistry to fabricate GO-functionalized membranes through two strategies: coating and blending. The two types of GO-functionalized membranes displayed comparable roughness, hydrophilicity, water permeability, and solute retention properties but different degrees of GO nanosheet exposure on the membrane surface. When in contact with the model bacterium, Escherichia coli, the GO-coated membrane exhibited enhanced biofouling resistance compared to that of the GO-blended membrane, as evidenced by lower viable cells in static adsorption experiments, and lower water flux decline and higher flux recovery in dynamic biofouling experiments. Moreover, the development of biofilm on the GO-coated membrane was also inhibited to a greater extent than on the GO-blended membrane. Taken together, our findings indicate the paramount importance of GO exposure on the membrane surface in conferring antibacterial activity and biofouling resistance, which should be considered in the future design of antibiofouling membranes using 2D nanomaterials.

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Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Functionalized Membranes: The Importance of Nanosheet Surface Exposure for Biofouling Resistance

Cheng

Kaneda

et al. 2019

Environ. Sci. Technol.

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“…They found that the maximum adhesion force between horizontally arranged GO sheets on the silicon (Si) wafer and bacteria was −0.78 nN. Besides that, even if modified GO with a hydrophilic polymer weakened the adhesion of bacteria, it still showed a maximum adhesion force of −0.11 nN to the bacteria . These values are far less than the results in Figure , which implies that there should be other components in the resultant force between GO and bacteria.…”

Section: Results and Discussionmentioning

confidence: 91%

“…Moreover, for the hydrophobic and adhesion force between GO and live bacteria, El-Kirat-Chatel et al and Wuolo-Journey et al found that a hydrophilic substrate showed lower bacterial adhesion force, while a hydrophobic substrate showed stronger bacterial adhesion due to hydrophobic interaction between bacterial adhesion and hydrophobic substrate. This implies that hydrophobic force can help cell attachment on hydrophobicity-increased rGO.…”

Section: Results and Discussionmentioning

confidence: 96%

Interaction between Biocompatible Graphene Oxide and Live Shewanella in the Self-Assembled Hydrogel: The Role of Physicochemical Properties

Jin

Pang

et al. 2020

ACS Appl. Bio Mater.

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Understanding the interaction of graphene materials with bacterial cells is crucial for exploiting their environmental applications. Meanwhile, knowledge on the mechanism of graphene oxide (GO) action to bacteria is still incomplete. This study focused on the inter-relationship of biocompatible GO and the well-known dissimilatory metal-reducing bacteria Shewanella, in view of the biographene hydrogel (BGH), a self-assembly of GO and live bacteria. The results showed that, among various inter-related physicochemical properties of GO, the sheet area determined the bacterial survival and the gelation potential with the same Shewanella strain. For the biocompatible GO sheet above 0.30 μm 2 , the larger the GO, the higher the speed of BGH assembling. Only 22 h was needed to obtain BGH using GO with an average area of 1.83 μm 2 (maximum in this study). The GO oxidation degree was found to be another critical factor affecting whether BGH formed or not, with a referential threshold of C/O > 1.75. Finally, surface force of GO was detected and correlated with the bacterial adhesion behavior for the first time, confirming that the large GO in the low oxidation state has high resultant force to attract bacteria. All these findings pave a promising way to develop a GO-bacteria complex like BGH to treat industrial wastewater in the future.

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“…Nonetheless, enhanced bacterial attachment compromises the bactericidal efficacy and causes severe fouling. Previous atomic force microscopy (AFM)-based studies revealed that the bacteria–GO interaction was predominantly repulsive due to the electrostatic and steric repulsion between deprotonated carboxylate groups in GO and the negatively charged cell surface. , On the other hand, the enhanced bacterial attachment to GO-coated organic and inorganic surfaces via hydrophobic interaction has been demonstrated. − These contradictory conclusions are mainly derived from the various parameters influencing the interfacial behaviors. It has been recognized that the chemical properties of the background electrolyte such as ionic strength govern the extent and mechanisms of bacterial attachment to surfaces. − Moreover, the lack of real-time and quantitative analysis of the bacterial deposition dynamics on GO surface also leads to divergent results from different investigators. ,, As bacterial attachment onto GO is critical in determining GO’s antibiofouling and antibacterial performance, it is crucial to reveal the effect of solution chemistry on the dynamic bacterial attachment processes on GO surface and develop a comprehensive understanding of bacteria–GO interaction.…”

Section: Introductionmentioning

confidence: 99%

“…Previous atomic force microscopy (AFM)-based studies revealed that the bacteria−GO interaction was predominantly repulsive due to the electrostatic and steric repulsion between deprotonated carboxylate groups in GO and the negatively charged cell surface. 11,12 On the other hand, the enhanced bacterial attachment to GO-coated organic and inorganic surfaces via hydrophobic interaction has been demonstrated. 13−15 These contradictory conclusions are mainly derived from the various parameters influencing the interfacial behaviors.…”

Section: ■ Introductionmentioning

confidence: 99%

Ionic Strength-Dependent Attachment of Pseudomonas aeruginosa PAO1 on Graphene Oxide Surfaces

Jing

Wang

et al. 2022

Environ. Sci. Technol.

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Graphene oxide (GO) is a widely used antimicrobial and antibiofouling material in surface modification. Although the antibacterial mechanisms of GO have been thoroughly elucidated, the dynamics of bacterial attachment on GO surfaces under environmentally relevant conditions remain largely unknown. In this study, quartz crystal microbalance with dissipation monitoring (QCM-D) was used to examine the dynamic attachment processes of a model organism Pseudomonas aeruginosa PAO1 onto GO surface under different ionic strengths (1–600 mM NaCl). Our results show the highest bacterial attachment at moderate ionic strengths (200–400 mM). The quantitative model of QCM-D reveals that the enhanced bacterial attachment is attributed to the higher contact area between bacterial cells and GO surface. The extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) theory and atomic force microscopy (AFM) analysis were employed to reveal the mechanisms of the bacteria–GO interactions under different ionic strengths. The strong electrostatic and steric repulsion at low ionic strengths (1–100 mM) was found to hinder the bacteria–GO interaction, while the limited polymer bridging caused by the collapse of biopolymer layers reduced cell attachment at a high ionic strength (600 mM). These findings advance our understanding of the ionic strength-dependent bacteria–GO interaction and provide implications to further improve the antibiofouling performance of GO-modified surfaces.

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Do graphene oxide nanostructured coatings mitigate bacterial adhesion?

Abstract: Graphene oxide (GO) is a biocidal nanomaterial, but is it also anti-adhesive? Here we show that GO-based coatings exhibiting low bacterial adhesion properties can be formed by edge-tethering GO nanosheets to hydrophilic polymer brushes.

Cited by 11 publications

References 69 publications

Graphene Oxide-Functionalized Membranes: The Importance of Nanosheet Surface Exposure for Biofouling Resistance

Graphene Oxide-Functionalized Membranes: The Importance of Nanosheet Surface Exposure for Biofouling Resistance

Interaction between Biocompatible Graphene Oxide and Live Shewanella in the Self-Assembled Hydrogel: The Role of Physicochemical Properties

Ionic Strength-Dependent Attachment of Pseudomonas aeruginosa PAO1 on Graphene Oxide Surfaces

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