The Non-Tariff Measures (NTMs) include a wide range of issues; those that cover food safety, animal health and plant protection, are known as Sanitary and Phytosanitary (SPS). SPS are a key element in the agrifood trade and a major challenge for trade policy and food safety. The application of the SPS is a dynamic and complex process due to the diversity of actors involved and the heterogeneity of objectives pursued: political, social, economic and environmental. The changing nature between countries of agrifood trade and methodology limitations and access to reliable and updated information increase the difficulty of estimating the impact generated by the application of NTMs. In this global scenario, with increasingly complex regulations and organizations that affect the rules of agricultural trade, it is necessary to know more about its operation.This research aims to improve the understanding of the implementation of SPS in the agrifood trade. It challenges the assumption that the application of MNA is directly or exclusively linked to technical issues or others related to food safety, aiming to investigate other drivers that influence its application. An analysis of the underlying factors that affect the implementation of SPS is carried out through two research questions: Q1) whether the implementation of NTMs is related to the elimination of trade tariffs, ultimately, the existence of policy substitution between NTMs and tariff; Q2) the application of NTMs is motivated by a systematic behaviour, guided by economic considerations and policies beyond the application of specific food notifications and health problems. The Q1 is studied in the countries of the South and East Mediterranean (SEMC) while Q2 is studied at the European Union (EU).The methodology used to address the first hypothesis, the policy substitution, begins with an analysis of the state of the art, continues with the classification into categories based on the use of NTMs and tariffs, and finally applies multiple regression models to test the hypothesis. To compare tariffs and NTMs, the research works with the Ad Valorem Equivalents (AVEs) established by Kee et al. (2009) and supported by an extensive literature. To address the second hypothesis, the source of information used is the RASFF (Rapid Alert System for Food and Feed) database. The methodology applied begins with the construction of a tool able to transform viii the descriptive information from the RASFF into the numerical coding of the Harmonized System (HS) of trade, to subsequently apply the Negative Binomial model (NB) and Zero-Inflated Negative Binomial model (ZINB), thus identifying the determinants of the notifications process included in the RASFF from several Member States (MS) studied. The thesis presents the four articles written throughout the investigation.The results indicate that: (i) there is need for pursuing a new approach in agrifood trade policies, beyond the economic sphere, to strengthen the partnership between the EU and other countries, particularly ...