Do farm-grown lungs breathe better?

Genuneit, Jon

doi:10.1136/thoraxjnl-2016-209280

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Age-dependent maturation of the immune system occurs after birth once the neonate encounters the antigen-rich external environment (22). The composition of the airway bacterial profile in utero and in early life is also important because exposure to a diverse bacterial mix, such as that found on traditional cattle farms, has been shown to protect from the development of allergy and asthma (23,24). The capacity of the adaptive immune system to induce memory responses is limited and is thought to gradually develop after early-life environmental exposure to microbes, pollutants, and allergens (25).…”

Section: Introductionmentioning

confidence: 99%

Inception of early-life allergen–induced airway hyperresponsiveness is reliant on IL-13 ⁺ CD4 ⁺ T cells

et al. 2018

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Airway hyperresponsiveness (AHR) is a critical feature of wheezing and asthma in children, but the initiating immune mechanisms remain unconfirmed. We demonstrate that both recombinant interleukin-33 (rIL-33) and allergen [house dust mite (HDM) or ] exposure from day 3 of life resulted in significantly increased pulmonary IL-13CD4 T cells, which were indispensable for the development of AHR. In contrast, adult mice had a predominance of pulmonary LinCD45CD90IL-13 type 2 innate lymphoid cells (ILC2s) after administration of rIL-33. HDM exposure of neonatal IL-33 knockout (KO) mice still resulted in AHR. However, neonatal CD4IL-13 KO mice (lacking IL-13CD4 T cells) exposed to allergen from day 3 of life were protected from AHR despite persistent pulmonary eosinophilia, elevated IL-33 levels, and IL-13 ILCs. Moreover, neonatal mice were protected from AHR when inhaled (an environmental bacterial isolate found in cattle farms, which is known to protect from childhood asthma) was administered concurrent with HDM. blocked the expansion of pulmonary IL-13CD4 T cells, whereas IL-13 ILCs and IL-33 remained elevated. Administration of mirrored the findings from the CD4IL-13 KO mice, providing a translational approach for disease protection in early life. These data demonstrate that IL-13CD4 T cells, rather than IL-13 ILCs or IL-33, are critical for inception of allergic AHR in early life.

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