Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation?

Peña, Fernando J.; Saravia, F.; Núñez-Martínez, I; Johannisson, Anders; Wallgren, Margareta; Rodriguez‐Martinez, Heriberto

doi:10.1016/j.anireprosci.2005.06.028

Cited by 73 publications

(49 citation statements)

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“…Differences in sperm quality post-thaw between boars were sometimes found (Peña et al, 2006) or not (Saravia et al, 2007). Inter-animal differences in their ability to sustain sperm cryopreservation had been associated to the SP-composition (Flowers, 2001;Novak et al, 2010), genetic factors (Thurston et al, 2002), or by the existence of distinct spermatozoa subpopulations in the ejaculate, in terms of their morphological characteristics (Thurston et al, 2001) or movement patterns (Cremades et al, 2005).…”

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confidence: 99%

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Siqueira

Wallgren

Hossain³

et al. 2011

Theriogenology

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Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction, 2011 , THERIOGENOLOGY, (75), 7, 1175 -1184 AbstractBoar sperm viability post-thaw differs depending on the ejaculate fraction used, with spermatozoa present in the first 10mL of the sperm-rich fraction, SRF (portion 1, P1, sperm-peak portion), showing the best cryosurvival in vitro when compared with spermatozoa from the rest of the ejaculate (second portion of the SRF plus the post-spermatic fraction), even when using simplified freezing routines. Such abilities apparently relate to the specific composition of seminal plasma in the P1 (glycoproteins, pH, and bicarbonate concentrations). However, spermatozoa from P1 have not been compared to the rest of spermatozoa in the SRF (SRF-P1, usually 30 to 40 mL of the SRF), which is routinely used for freezing.Such comparison of P1 vs SRF-P1 was hereby done, in terms of sperm kinematics (QualiSperm TM system), membrane integrity (SYBR-14/PI), acrosome integrity (FITC PNA/PI), and sperm membrane stability (Annexin-V), explored using flow cytometry. As well, total protein concentration and the proteomics of the seminal plasma (SP) of either portion of the SRF were studied using two-dimensional electrophoresis (2DE), mass fingerprinting (MALDI-TOF) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of selected peptides. The SRF portions were weekly collected from four mature boars (4-5 replicates per boar, sperm concentration: P1-1.86±0.20, SRF-P1-1.25±0.14 ×10 9 spz/mL) and processed using a quick freezing method in MiniFlatPacks (MFP). Sperm motility post-thaw reached 50%, without differences between portions of the SRF, but with clear inter-boar variation. Neither did plasma membrane or acrosome integrity differ (ns) between fractions. These results indicate that there are no differences in cryosurvival after quick freezing of boar spermatozoa derived from either of the two portions of the SRF. While P1 and SRF-P1 clearly differed in relative total protein contents, as expected, they displayed very similar protein profiles as assessed by 2DE and mass spectrometry (tryptic peptide mass fingerprint analysis and CID-MS/MS), indicating a similar emission of epididymal protein content.3

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confidence: 99%

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Siqueira

Wallgren

Hossain³

et al. 2011

Theriogenology

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“…A high rate of morphologic abnormality in the ejaculate has been correlated with lowered fertility [2][3][4][5][6]. The morphology and motility may be related to sperm survival and ability to fertilize the ovum [7,8]. We hypothesized that the morphology and motility of spermatozoa in semen analysis are interrelated with each other and with total sperm count in the ejaculate.…”

Section: Introductionmentioning

confidence: 99%

Alterations in the morphology and motility of spermatozoa: relation with total sperm count

Karabulut¹,

Tekin²

2013

Pau Med J

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Purpose: The aim of this study was to investigate the differences in sperm morphology and motility in patients with different sperm counts, and to search the influence of sperm morphology over sperm motility. Materials and methods:We evaluated semen analysis of 1174 males admitted to infertility clinic using "Makler counting camber" for evaluation of number and motility, and strict criteria (Kruger-Tyberg) for the morphological assessment. According to total sperm count, patients were divided into three groups; group I (n=119) had <5x10 6 /mL, group II (n=125) had 5-15x10 6 /mL, and group III (n=930) had ≥15x10 6 /mL total sperm count. The groups were compared in terms of motility, morphology and the distribution of sperm abnormalities. Results: Sperm motility was significantly lower in groups I-II compared to group III (p<0.01). In the semen analysis, normal sperm morphology above 4% was detected in 7.1% of group II, 17.5% of group III, and none in group I (p<0.01). Significantly less tail defects were detected in group III compared to others (p<0.01). Progressive sperm motility significantly correlated with morphology (r=0.38, p<0.001). Among morphologic abnormalities, tail problems were highly correlated with nonmotile sperm count (r=0.30, p<0.001). Tail defects correlated positively with midpiece (r=0.24, p<0.001), and negatively with head defects (r=-0.71, p<0.001). Normal sperm morphology had a negative relation with tail and midpiece defects (r=-0.20, p <0.001 and r=-0.30, p<0.001 respectively). Conclusion: Sperm motility and morphology were deteriorated with the decreasing sperm count in subfertile population. The chance of finding normal morphology above the critical value of 4% diminishes in cases with sperm counts less than 5x10 6 /mL. Therefore, it is logical to canalize these patients directly to assisted reproductive technologies.Pam Med J 2013;6(1):1-4

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“…Many attempts have been made to reduce the alterations produced in sperm cells during this process (Breininger et al 2005, Peñ a et al 2005, Bathgate et al 2006, but the results are still far from optimal. Success in the cryopreservation of boar sperm cells might be aided by testing this technique in Iberian pigs, an older breed, which has not been selected for freezability and for which no studies have been reported.…”

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

Sancho

Casas²,

Ekwall³

et al. 2007

Reproduction

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This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the 'Entrepelado' and 'Lampiñ o' breeds. The samples were suspended in a commercial extender and refrigerated to 17 8C for transport to the laboratory (step A), where they were further extended with a lactose-egg yolk-based extender and chilled to 5 8C (step B) prior to freezing in the presence of glycerol (3%). Spermatozoa were assessed for plasma membrane integrity and sperm motility at each of the steps, including post-thaw (step C). Aliquots were also prepared for immunocytochemical localisation of the sugar transporters (fixed and thin smears for transmission and scanning electron microscopy levels respectively) and for SDS-PAGE electrophoresis and subsequent western blotting, using the same antibodies (rabbit anti-GLUT-3 and anti-GLUT-5 polyclonal antibodies). The results showed lower percentages of progressively motile spermatozoa at step C in both breeds, while the percentage of live spermatozoa was significantly lower only in the 'Entrepelado' breed. The results obtained from electron microscopy clearly showed that Iberian boar spermatozoa expressed the hexose transporters, GLUT-3 and GLUT-5. The pattern of expression, in terms of location and concentration, was characteristic in each case but, in the case of isoform GLUT-5, it remained constant during the different steps of freezing-thawing protocol. These results indicate that cryopreservation affects the status of sperm cells of Iberian boars by altering the distribution of some membrane receptors and decreasing the percentage values of parameters linked to sperm quality.

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Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation?

Cited by 73 publications

References 20 publications

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Alterations in the morphology and motility of spermatozoa: relation with total sperm count

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

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