Do cultural conditions induce differential protein expression: Profiling of extracellular proteome of Aspergillus terreus CM20

Saritha, M.; Singh, Surender; Tiwari, Rameshwar; Goel, Renu; Nain, Lata

doi:10.1016/j.micres.2016.06.006

Cited by 16 publications

(6 citation statements)

References 67 publications

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“…The data further show that the fungal response to growth on lignocellulose includes induction of peptidases predicted to be secreted. Although this has been reported previously 20 , the exact role of peptidases in lignocellulose degradation is not understood. It has been speculated that peptidases could degrade plant cell wall proteins, thereby giving increased access for GHs to degrade polysaccharides 34 .…”

Section: Resultsmentioning

confidence: 77%

“…In this study, we present a quantitative comparison of the secretomes of five lignocellulose-degrading filamentous fungi. Aspergillus terreus (Ascomycota; Eurotiomycetes; Eurotiales) is a saprophytic ascomycete found in soil and compost and is known to degrade a wide range of plant biomass 20 . Trichoderma reesei (Ascomycota; Sordariomycetes; Hypocreales) is a mesophilic ascomycete, famed for degrading textiles during World War II, and is widely used in the enzyme industry due to its high protein secretion capacity 14 .…”

Section: Introductionmentioning

confidence: 99%

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Quantitative comparison of the biomass-degrading enzyme repertoires of five filamentous fungi

Arntzen

Bengtsson

Várnai

et al. 2020

Sci Rep

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The efficiency of microorganisms to degrade lignified plants is of great importance in the Earth’s carbon cycle, but also in industrial biorefinery processes, such as for biofuel production. Here, we present a large-scale proteomics approach to investigate and compare the enzymatic response of five filamentous fungi when grown on five very different substrates: grass (sugarcane bagasse), hardwood (birch), softwood (spruce), cellulose and glucose. The five fungi included the ascomycetes Aspergillus terreus, Trichoderma reesei, Myceliophthora thermophila, Neurospora crassa and the white-rot basidiomycete Phanerochaete chrysosporium, all expressing a diverse repertoire of enzymes. In this study, we present comparable quantitative protein abundance values across five species and five diverse substrates. The results allow for direct comparison of fungal adaptation to the different substrates, give indications as to the substrate specificity of individual carbohydrate-active enzymes (CAZymes), and reveal proteins of unknown function that are co-expressed with CAZymes. Based on the results, we present a quantitative comparison of 34 lytic polysaccharide monooxygenases (LPMOs), which are crucial enzymes in biomass deconstruction.

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Section: Resultsmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Quantitative comparison of the biomass-degrading enzyme repertoires of five filamentous fungi

Arntzen

Bengtsson

Várnai

et al. 2020

Sci Rep

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“…Note that many high-throughput studies report proteins with transmembrane (TM) domains as part of the secretome but a few studies have specifically identified cell membrane and cell wall associated proteins along with the secretome 30 , 36 . Our compilation of high-throughput proteomic studies led to experimentally identified lists of 437, 364, 454, 96, 469 and 171 secreted proteins in A. fumigatus 27 – 41 , A. flavus 30 , 42 – 45 , A. niger 30 , 46 – 58 , A. terreus 30 , 59 , 60 , A. nidulans 58 , 61 – 65 and A. oryzae 57 , 66 – 73 , respectively (Methods; Supplementary Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

Vivek-Ananth

Mohanraj

Vandanashree

et al. 2018

Sci Rep

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Aspergillus fumigatus and multiple other Aspergillus species cause a wide range of lung infections, collectively termed aspergillosis. Aspergilli are ubiquitous in environment with healthy immune systems routinely eliminating inhaled conidia, however, Aspergilli can become an opportunistic pathogen in immune-compromised patients. The aspergillosis mortality rate and emergence of drug-resistance reveals an urgent need to identify novel targets. Secreted and cell membrane proteins play a critical role in fungal-host interactions and pathogenesis. Using a computational pipeline integrating data from high-throughput experiments and bioinformatic predictions, we have identified secreted and cell membrane proteins in ten Aspergillus species known to cause aspergillosis. Small secreted and effector-like proteins similar to agents of fungal-plant pathogenesis were also identified within each secretome. A comparison with humans revealed that at least 70% of Aspergillus secretomes have no sequence similarity with the human proteome. An analysis of antigenic qualities of Aspergillus proteins revealed that the secretome is significantly more antigenic than cell membrane proteins or the complete proteome. Finally, overlaying an expression dataset, four A. fumigatus proteins upregulated during infection and with available structures, were found to be structurally similar to known drug target proteins in other organisms, and were able to dock in silico with the respective drug.

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“…terreus 30,59,60 , A. nidulans 58,[61][62][63][64][65] and A. oryzae 57,[66][67][68][69][70][71][72][73] , respectively (Methods; Supplementary Table S1).…”

Section: Introductionmentioning

confidence: 99%

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

Vivek-Ananth

Mohanraj

Vandanashree

et al. 2017

Preprint

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Aspergillus fumigatus and multiple other Aspergillus species cause a wide range of lung infections, collectively termed aspergillosis. Aspergilli are ubiquitous in environment with healthy immune systems routinely eliminating inhaled conidia, however, Aspergilli can become an opportunistic pathogen in immune-compromised patients. The aspergillosis mortality rate and emergence of drug-resistance reveals an urgent need to identify novel targets. Secreted and cell membrane proteins play a critical role in fungal-host interactions and pathogenesis. Using a computational pipeline integrating data from high-throughput experiments and bioinformatic predictions, we have identified secreted and cell membrane proteins in ten Aspergillus species known to cause aspergillosis. Small secreted and effector-like proteins similar to agents of fungal-plant pathogenesis were also identified within each secretome. A comparison with humans revealed that at least 70% of Aspergillus secretomes has no sequence similarity with the human proteome. An analysis of antigenic qualities of Aspergillus proteins revealed that the secretome is significantly more antigenic than cell membrane proteins or the complete proteome.Finally, overlaying an expression dataset, four A. fumigatus proteins upregulated during infection and with available structures, were found to be structurally similar to known drug target proteins in other organisms, and were able to dock in silico with the respective drug.

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Do cultural conditions induce differential protein expression: Profiling of extracellular proteome of Aspergillus terreus CM20

Cited by 16 publications

References 67 publications

Quantitative comparison of the biomass-degrading enzyme repertoires of five filamentous fungi

Quantitative comparison of the biomass-degrading enzyme repertoires of five filamentous fungi

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

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