Do Anti-Oxidants and Local Anaesthetics Extend Pollen Longevity during Dry Storage?

Hoekstra, Folkert A.; Barten, J. H. M.

doi:10.1007/978-1-4613-8622-3_54

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high level of linolenic acid (C18 : 3) niay have led to the production of hydroperoxides derived from linolenic acid, which affect membrane integrity in general (Pauls & Thompson, 1984). The absolute amounts of free fatty acids, known for their fusogenie properties (Ahkong et at., 1973), are low compared to those encountered during dry ageing of other pollen speeies (Hoekstra & Barten, 1986). The possibility cannot be excluded, therefore, that these egradative processes are involved in the generally ow desiccation tolerance of Gramineae pollen.…”

Section: Effects Of Phospholipid Composition On Sensitivity To Desiccmentioning

confidence: 89%

Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents

Hoekstra

Crowe

1989

Plant Cell & Environment

127

View full text Add to dashboard Cite

Abstract. Two pollen species from the Gramineae family were investigated as to their differential sensitivity to desiccation. Germination in vivo ceased to occur after previous drying to 7–8% moisture content in Zea mays and 3% in Pennisetum typhoides. The reduced vitality coincided with extensive membrane damage, as evidenced by the considerable leakage of fluorescein and K+ from the prehumidified grains into liquid media. Further experiments were undertaken to investigate the basis for this interspecific difference: (1) phospholipid analyses revealed little difference in composition and content both before and after drying; (2) free fatty acid contents increased in both species upon drying but levels were generally low; and (3) sucrose was the sole soluble carbohydrate found in both species. Fresh maize pollen contained 5% sucrose, as compared to 14% in Pennisetum pollen. During slow drying (8h) this level went up to 12% and 17%, respectively. Drying corn pollen in the cold (2°C), or at high rate, limited sucrose levels and affected the resistance to drying. The authors conclude that survival of dehydration is correlated with the presence of sucrose. Finally, they tested the ability of sucrose to preserve dry liposomes prepared from phospholipids purified from the two species. When liposomes were dried in the presence of sucrose, fusion and leakage could largely be prevented at mass ratio's of sugar to lipid of > 4. Trehalose was also effective but myo‐inositol was not. No species differences were observed. The authors suggest that the presence of sucrose is a key factor in preserving membranes in dry pollen.

show abstract

Section: Effects Of Phospholipid Composition On Sensitivity To Desiccmentioning

confidence: 89%

Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents

Hoekstra

Crowe

1989

Plant Cell & Environment

127

View full text Add to dashboard Cite

show abstract

“…In viable, nonaged Typha pollen, lysolipids occur below the limit of detection, and free fatty acids comprise less than 0. 1% of the dry weight (23). The remaining major CH2-sources are the phospholipids and storage fats, of which we determined the contents to amount to 5.3 and 8.0% of the dry weight, respectively (cJf 2).…”

Section: Assignment Of the Absorption Bands In Pollen Tomentioning

confidence: 99%

Effect of Sucrose on Phase Behavior of Membranes in Intact Pollen of Typha latifolia L., as Measured with Fourier Transform Infrared Spectroscopy

Hoekstra

Crowe²,

Crowe³

1991

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

In previous studies, we have shown that the temperature dependent vibrational frequency of the CH2 stretch in hydrocarbons in intact pollen grains can be recorded with Fourier transform infrared spectroscopy and used to measure phase transition temperatures (Tm) in these hydrocarbons. Circumstantial evidence was provided that the major contribution to the signal seen in these samples was from membrane phospholipids, and that sucrose in the dry pollen grains reduced Tm of those phospholipids. In the current study, we clarify why a major constituent of the pollen grains, neutral lipids contained in discrete lipid droplets, does not contribute significantly to the signal. Further, we have isolated membranes from the pollen and show that Tm in the isolated membranes rises from -60C in the hydrated membranes to 580C when the membranes are dried without the addition of sucrose. However, when the isolated membranes are dried in the presence of increasing amounts of sucrose, Tm fell steadily, reaching a minimal value of 310C, a figure in good agreement with that seen in the intact pollen grains. The amount of sucrose required to depress Tm maximally in these membranes is also apparently in agreement with that found in the intact pollen, suggesting that sucrose depresses Tm in the pollen.Certain disaccharides, such as trehalose and sucrose, apparently play a role in the acquisition of desiccation tolerance in living organisms. This suggestion was based on the occurrence of high levels of disaccharides in desiccation-tolerant organisms (1,8,24,25,28) and on studies in vitro of their protective effect on isolated membranes (7), liposomal membranes (15, 31), and proteins (4, 5) during drying and rehydration. Trehalose is the predominant disaccharide in organisms such as yeasts, fungi, the desert resurrection plant Selaginella (19), and microscopic animals (8), whereas anhydrobiotic organs of higher plants accumulate mainly sucrose (1,24,25)

show abstract

Optimization of isolation and storage of sperm cells from pollen of perennial ryegrass (Lolium perenne L.)

Maas

Zaal

Jong

et al. 1993

Sexual Plant Reprod

View full text Add to dashboard Cite

Do Anti-Oxidants and Local Anaesthetics Extend Pollen Longevity during Dry Storage?

Cited by 4 publications

References 15 publications

Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents

Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents

Effect of Sucrose on Phase Behavior of Membranes in Intact Pollen of Typha latifolia L., as Measured with Fourier Transform Infrared Spectroscopy

Optimization of isolation and storage of sperm cells from pollen of perennial ryegrass (Lolium perenne L.)

Contact Info

Product

Resources

About