Dnd is required for primordial germ cell specification in Oryzias celebensis

Zhu, Tianyu; Liu, Gui; Zhu, Yefei; Yu, Li; Li, Mingyou

doi:10.1016/j.gene.2018.08.068

Cited by 29 publications

(16 citation statements)

References 53 publications

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“…In model fish like zebrafish and medaka, morpholino-based knockdown of the dnd transcript results in GCF individuals, while co-injection of dnd mRNA and morpholino targeting the dnd transcripts can rescue the PGCs 21,27,28 . These studies, however, were not suited to investigate long-term effects beyond the embryonic stages, since morpholino-based knockdown has only transient effects.…”

Section: Discussionmentioning

confidence: 99%

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

Güralp

Skaftnesmo

Kjærner-Semb

et al. 2020

Sci Rep

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Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the dead-end (dnd) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic salmon dnd crispants. To achieve this, we co-injected the wild-type (wt) variant of salmon dnd mRNA together with CRISPR-Cas9 constructs targeting dnd into 1-cell stage embryos. We found that rescued one-year-old fish contained germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. The method presented here opens a possibility for large-scale production of germ-cell free Atlantic salmon offspring through the genetically sterile broodstock which can pass the sterility trait on the next generation.

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Section: Discussionmentioning

confidence: 99%

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

Güralp

Skaftnesmo

Kjærner-Semb

et al. 2020

Sci Rep

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“…The expression patterns of key PGC markers 37,38 , including nanos2, nanos3, and dnd1, along with pluripotency marker nanog in E-GSC, confirmed the pluripotency of E-GSC cells. nanos3 was expressed slightly higher in E-GSC than in L-GSC, indicating the relatively higher stemness of E-GSC in comparison with L-GSC (Fig.…”

Section: Two Developmental Trajectories Of Five Subtypes Of Germline Stem Cellsmentioning

confidence: 58%

Cellular fate of intersex differentiation

et al. 2021

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Infertile ovotestis (mixture of ovary and testis) often occurs in intersex individuals under certain pathological and physiological conditions. However, how ovotestis is formed remains largely unknown. Here, we report the first comprehensive single-cell developmental atlas of the model ovotestis. We provide an overview of cell identities and a roadmap of germline, niche, and stem cell development in ovotestis by cell lineage reconstruction and a uniform manifold approximation and projection. We identify common progenitors of germline stem cells with two states, which reveal their bipotential nature to differentiate into both spermatogonial stem cells and female germline stem cells. Moreover, we found that ovotestis infertility was caused by degradation of female germline cells via liquid–liquid phase separation of the proteasomes in the nucleus, and impaired histone-to-protamine replacement in spermatid differentiation. Notably, signaling pathways in gonadal niche cells and their interaction with germlines synergistically determined distinct cell fate of both male and female germlines. Overall, we reveal a cellular fate map of germline and niche cell development that shapes cell differentiation direction of ovotestis, and provide novel insights into ovotestis development.

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“…In teleosts, the maternal factors such as tdrd6 safeguards proper germ plasm segregation ( Roovers et al, 2018 ), while, dnd ( Hong et al, 2016 ; Zhu et al, 2018 ) and dazl ( Li et al, 2016 ) are necessary for maintaining PGC identity, with nanos1 ( Koprunner et al, 2001 ), and vasa ( Braat et al, 2001 ; Li et al, 2009 ) providing signals for their proliferation and correct migration. The maternal accumulation of these markers in ova supports early germline sequestration in G. holbrooki with comparable functions reported for other fish models.…”

Section: Discussionmentioning

confidence: 99%

“…Typically, PGCs undergo several developmental stages before transforming into functional germ cells. These begin with PGC specification regulated by parental derivates ( Hong et al, 2016 ; Zhu et al, 2018 ) or epigenetic reprogramming ( Yabuta et al, 2006 ; Saitou and Yamaji, 2012 ), followed by mobility acquisition to reach gonad anlage ( Richardson and Lehmann, 2010 ; Paksa and Raz, 2015 ), and eventually attaining sexual identity, influenced by genetic/intrinsic ( Devlin and Nagahama, 2002 ; Matsuda, 2005 ) or environmental/extrinsic ( Baroiller et al, 2009 ) cues. Cumulative evidence suggests that each of these stages are governed by a group of molecules which recruit epigenetic machinery ( Andersen et al, 2012 ), RNA interference apparatus ( Giraldez et al, 2006 ) and/or signaling pathways ( Boldajipour et al, 2008 ) safeguarding PGCs from somatic differentiation ( Gross-Thebing et al, 2017 ), ectopic migration and apoptosis ( Lai et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

Razmi

Patil

2022

Front. Cell Dev. Biol.

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Metazoans exhibit two modes of primordial germ cell (PGC) specification that are interspersed across taxa. However, the evolutionary link between the two modes and the reproductive strategies of lecithotrophy and matrotrophy is poorly understood. As a first step to understand this, the spatio-temporal expression of teleostean germ plasm markers was investigated in Gambusia holbrooki, a poecilid with shared lecitho- and matrotrophy. A group of germ plasm components was detected in the ovum suggesting maternal inheritance mode of PGC specification. However, the strictly zygotic activation of dnd-β and nanos1 occurred relatively early, reminiscent of models with induction mode (e.g., mice). The PGC clustering, migration and colonisation patterns of G. holbrooki resembled those of zebrafish, medaka and mice at blastula, gastrula and somitogenesis, respectively—recapitulating features of advancing evolutionary nodes with progressive developmental stages. Moreover, the expression domains of PGC markers in G. holbrooki were either specific to teleost (vasa expression in developing PGCs), murine models (dnd spliced variants) or shared between the two taxa (germline and somatic expression of piwi and nanos1). Collectively, the results suggest that the reproductive developmental adaptations may reflect a transition from lecithotrophy to matrotrophy.

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Dnd is required for primordial germ cell specification in Oryzias celebensis

Cited by 29 publications

References 53 publications

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

Cellular fate of intersex differentiation

Primordial Germ Cell Development in the Poeciliid, Gambusia holbrooki, Reveals Shared Features Between Lecithotrophs and Matrotrophs

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