DNAzyme crosslinked hydrogel: a new platform for visual detection of metal ions

Abstract

We propose the use of DNAzyme as a crosslinker of hydrogel to develop a catalytic platform for the sensing of metal ions. The DNAzyme crosslinked hydrogel can undergo gel-sol transition in response to Cu(2+) ions, which enables sensitive visual detection of Cu(2+) by observing the release of pre-trapped AuNPs.

pH‐Stimulated DNA Hydrogels Exhibiting Shape‐Memory Properties

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by DNA strand-displacement, [ 27 ] temperature, [ 28 ] enzyme [ 29 ] -or DNAzyme [ 30 ] -catalyzed cleavage of the cross-linking bridges. Also, the reversible and cyclic transitions of DNA between hydrogel and solution phases using pH changes [ 31 ] or by the K + -stimulated formation of G-quadruplex cross-bridges and their separation by crown-ethers [ 32 ] were demonstrated.

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An electrochemical biosensor for the sensitive detection of specific DNA based on a dual-enzyme assisted amplification

“…The electrode was extensively washed, and placed in a TMB solution for electrochemical measurements. EIS measurements were carried out in 10 mM Fe(CN) 6 3À/4À containing 20 mM Tris-HCl (pH 8.0) and 20 mM NaCl with a frequency range from 0.1 to 10 kHz.…”

“…Consequently, molecular biology, electrochemistry, spectroscopy, and colorimetry have all been utilized towards the detection of nucleic acids [4][5][6]. Among these, the electrochemical sensor (Esensor) has attracted increasing attention owing to its high sensitivity, low cost, simplicity and portability [7,8].…”

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

“…In addition to using aptamers, the Yang group recently used DNAzymes as a crosslinker to create a gel [58]. This DNAzyme cleaves its DNA substrate in the presence of Cu 2+ [59,60].…”