DNAzyme based gap-LCR detection of single-nucleotide polymorphism

Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity

“…The most popular method is a colorimetric one, where PMDNAzyme catalyzes an oxidation of 2,2 0 -azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) accompanying an increase of absorbance of the substrate solution in the range 414 to 420 nm [11,14,20,25]. It should be noted that ABTS is not an ideal substrate because ABTS + forming on the oxidation of ABTS is not a stable compound and quickly disproportionates with the production of colorless compounds [26,27].…”

Timing readout in paper device for quantitative point-of-use hemin/G-quadruplex DNAzyme-based bioassays

“…The sensitive, specific detection of a wide range of analytes, including metal ions (Hua et al, 2012;Li et al,2013;Liu et al, 2014;Yang et al, 2010;Wang and Irudayaraj, 2011;Wang et al, 2012;Wang et al, 2014c), small molecules (Garai-Ibabe et al, 2014;Wang et al, 2012;Wang et al, 2014g), DNAs (Wang et al, 2011;Wang et al, 2013;Wang et al, 2014a;Zhou et al, 2013), micro RNAs (Wen et al, 2012), proteins (Fu et al, 2011;He et al, 2012;Hou et al, 2014;Huang et al, 2013;Wang et al, 2014f;Tang et al, 2012;Zong et al, 2014), and bacterial pathogens (Gomez et al, 2014), implies the great potential of peroxidase-mimicking DNAzymes in broad applications ranging from medical diagnosis, environmental monitoring to food safety testing. These DNAzymes can effectively catalyze H 2 O 2 -mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (Fu et al, 2011;Gomez et al, 2014;He et al, 2012;Huang et al, 2013;Wang et al, 2012;Wang et al, 2014a;Wen et al, 2012;Tang et al,2012;Zhou et al, 2013), 3,3',5,5'-tetrazmethyl benzidinesulfate (TMB) (Yang et al, 2010), or luminol (Wang et al, 2011;Zong et al, 2014). As such, the existing quantitative hemin/G-quadruplex DNAzyme-based bioassays generally transducer recognition chemistry using several techniques, with spectrophotometry (Fu et al, 2011;…”

“…These DNAzymes can effectively catalyze H 2 O 2 -mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (Fu et al, 2011;Gomez et al, 2014;He et al, 2012;Huang et al, 2013;Wang et al, 2012;Wang et al, 2014a;Wen et al, 2012;Tang et al,2012;Zhou et al, 2013), 3,3',5,5'-tetrazmethyl benzidinesulfate (TMB) (Yang et al, 2010), or luminol (Wang et al, 2011;Zong et al, 2014). As such, the existing quantitative hemin/G-quadruplex DNAzyme-based bioassays generally transducer recognition chemistry using several techniques, with spectrophotometry (Fu et al, 2011;Gomez et al, 2014;He et al, 2012;Huang et al, 2013;Tang et al, 2012;Wang et al, 2012;Wang et al,2014a;Wen et al, 2012;Yang et al, 2010;Zhou et al, 2013) and chemiluminometry (Wang et al, 2011;Zong et al, 2014) being the two most common types. Other methods established recently include fluorescence (Hua et al, 2012;Liu et al, 2014), electrochemistry (Hou et al, 2014;Li et al, 2013;Wang et al, 2013;Wang et al, 2014c;Wang et al, 2014f;Wang et al, 2014g) and Raman scattering (Wang and Irudayaraj, 2011) techniques.…”

Real-time fluorescence ligase chain reaction for sensitive detection of single nucleotide polymorphism based on fluorescence resonance energy transfer

“…However, the detection of LCR products is challenging because the detection of LCR products generally requires separation by electrophoresis (Zhang et al, 2014). Recently a great deal of effort has been devoted to develop homogeneous detection technologies for monitoring LCR products without requirement of electrophoresis separation, such as conjugated polymer-based fluorescence resonance energy transfer (FRET) (Cheng et al, 2012), chemiluminescence resonance energy transfer (CRET) (Bi et al, 2015) and DNAzyme-based detection (Zhou et al, 2013). However, these methods also require one or multiple steps after LCR amplification.…”