DNase l-hypersensitive sites in the chromatin of rat growth hormone gene locus and enhancer activity of regions with these sites

Aizawa, Akira; Yoneyama, Tadashi; Kazahari, Koji; Ono, M.

doi:10.1093/nar/23.12.2236

Cited by 17 publications

(21 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No DHS at +6.7 kb found in liver and GC cells 1191 could be detected in liver-derived BRL cells (data not shown). DHS specific for GC and 235 cells was found at +8.6 kb [19]; none was observed from this site up to the +21.7-kb region (Fig. 2, and data not shown).…”

Section: Cosmid Clones and Restriction Mapmentioning

confidence: 83%

“…Buffalo rat liver-derived BRL cells were obtained from Riken Cell Bank (Tsukuba, Japan) and grown in Dulbecco's modified minimal essential medium containing 10% fetal bovine serum (JRH Biosciences). Isolation of nuclei, DNase I digestion and Southern hybridization were carried out as previously described [19].…”

Section: Methodsmentioning

confidence: 99%

“…Using GC (somatotropinf, prolactin-), 235 (somatotropin-, prolactin+) and liver (somatotropin--, prolactin-) cell nuclei, DHS of the rat somatotropin gene locus from -7 kb to -21 kb region were examined [19]. The features of this region, shown in Fig.…”

Section: Cosmid Clones and Restriction Mapmentioning

confidence: 99%

“…DNase I-hypersensitive sites (DHS) specific for somatotropin-producing cells were previously shown to be located exclusively in the -2 kb to +9 kb region [Aizawa, A., Yoneyama, T., Kazahari, K. & Ono, M. (1995) Nucleic Acids Res. 23, 2236.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Chromatin Structure of the Rat Somatotropin Gene Locus and Physical Linkage of the Rat Somatotropin Gene and Skeletal‐Muscle Sodium‐Channel Gene

Kazahari

Sano

Matsuura

et al. 1997

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

Cosmid clones from -32 kb to +74 kb region of the rat somatotropin gene locus were isolated for examination of the chromatin structure in the region from -39 kb to +47 kb by DNase I-sensitivity analysis using rat pituitary-derived GC (somatotropin+, prolactin-), and 235 (somatotropin-, prolactin+) cells, and liver-derived BRL (somatotropin-, prolactin-) cells. DNase I-hypersensitive sites (DHS) specific for somatotropin-producing cells were previously shown to be located exclusively in the -2 kb to +9 kb region [Aizawa, A., Yoneyama, T., Kazahari, K. & Ono, M. (1995) Nucleic Acids Res. 23, 2236. No other DHS having this specificity was found in the region examined in this study. Except for these and two other DHS located in a cluster in this region, no DHS could be found from -23 kb to +22 kb. DHS having no or less cell-type specificity were scattered about in the -39 kb to -23 kb and +22 kb to +47 kb regions. The polyadenylation site of the human skeletal-muscle Nachannel a-subunit gene has been shown present 22 kb upstream from the somatotropin gene [BennaniBaiti, I. M., Jones, B. K., Liebhaber, S. A. & Cooke, N. E. (1995) Cenornics 29, 647-6521. Polyadenylation site of the rat skeletal-muscle Na-channel gene was shown in this study to be at -15.7 kb. The skeletal-muscle Na-channel gene was specifically expressed in skeletal-muscle cells but not in somatotropin-producing cells, and thus the boundary region that ensures the cell-type-specific expression of each gene would appear to be situated between two genes. The region prerequisite for cell-type-specific expression of the rat soinatotropin gene was estimated based on the present findings.Keywords: chromatin; deoxyribonuclease I ; gene expression; sodium channel; somatotropin.Somatotropin and prolactin are typical vertebrate pituitary hormones. Similarity in amino acid sequence and gene organization suggests that the genes coding for these hormones are likely to have evolved from a common ancestor [I, 21. Somatotropin and prolactin are produced in somatotrophs and lactotrophs, respectively, both of which differentiate from Ratlike's pouch that arises from the oral ectoderm at the roof of the embryonic mouth [3, 41. The expression of somatotropin and prolactin in these tissues has been studied as a model system for elucidating the mechanism of cell-type-specific gene expression in vertebrates.Transient transfection studies indicated the region required for the cell-type-specific expression of the rat somatotropin gene to be as much as 235 bp upstream from the transcription-start site [ 5 ] . Two Pit-1/GHF-1 (referred as Pit-I in this article) binding sites, Spl and zinc-finger-protein factor(Zn-I5)-binding sites and the thyroid-hormone-responsive element were identified in this region 16, 71. Though transient transfection study can delimit the region required for cell-type-specific gene expression, in most transgenic mice studies, this region cannot confer celltype-specific gene expression either quantitatively or qualita-

show abstract

Section: Cosmid Clones and Restriction Mapmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Cosmid Clones and Restriction Mapmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Chromatin Structure of the Rat Somatotropin Gene Locus and Physical Linkage of the Rat Somatotropin Gene and Skeletal‐Muscle Sodium‐Channel Gene

Kazahari

Sano

Matsuura

et al. 1997

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…1, 3). 9,13,14) One possible explanation for reduced content of target sequences in DNase I-treated DNA may be disruption of the target sequence due to the presence of DHS in the target. For example, most DHS are cut by treatment of the nuclei with a lower concentration of DNase I.…”

Section: Conditions For Dnase I Treatment Of Nucleimentioning

confidence: 99%

State of Chromatin Sensitivity to DNase I in the Rat Ig-.BETA./Growth Hormone Locus Determined by Real-Time PCR

Osano

Otsuka

Ono

2004

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

Using Ig-b b and growth hormone producing cells with liver-derived cells for controls, sensitivity of chromatin to DNase I was measured by real-time PCR at eleven targets in rat Ig-b b/growth hormone locus where four cell type-specific genes and two ubiquitously expressed genes are present in a compact 88-kb region. Chromatin situated at the promoter of actively-transcribed gene and placed at cell type-specific DNase I hypersensitive sites with enhancer activity was sensitive to DNase I. In the case of inactive gene, chromatin located in these regions was resistant to DNase I. Unexpectedly, however, chromatin placed in the transcribed intron was resistant to DNase I in two genes. DNase I sensitive chromatin was shown not to distribute locus-widely but rather to localize at the promoter and the enhancer of actively-transcribed genes in this locus.

show abstract

Regulation of Growth Hormone Gene Expression

Cooke

Liebhaber

1999

Comprehensive Physiology

View full text Add to dashboard Cite

The sections in this article are: Transcriptional Controls Growth Hormone Gene Organization Activation of Proximal Promoter Elements of the Growth Hormone Gene in Somatotropes Structure and Function of the Proximal Promoter Activation of Placentally Expressed Human Growth Hormone Genes Role of Silencer Elements Genetic Evidence for Transcriptional Control and Developmental Pathways Posttranscriptional Controls Alternative Splicing of Human Growth Hormone Genes Alternative Splicing of the Bovine Growth Hormone Gene m RNA Stability

show abstract

DNase l-hypersensitive sites in the chromatin of rat growth hormone gene locus and enhancer activity of regions with these sites

Cited by 17 publications

References 46 publications

Chromatin Structure of the Rat Somatotropin Gene Locus and Physical Linkage of the Rat Somatotropin Gene and Skeletal‐Muscle Sodium‐Channel Gene

Chromatin Structure of the Rat Somatotropin Gene Locus and Physical Linkage of the Rat Somatotropin Gene and Skeletal‐Muscle Sodium‐Channel Gene

State of Chromatin Sensitivity to DNase I in the Rat Ig-.BETA./Growth Hormone Locus Determined by Real-Time PCR

Regulation of Growth Hormone Gene Expression

Contact Info

Product

Resources

About