DNase I hypersensitivity analysis of the human CCAAT enhancer binding protein ε (C/EBPε) gene

Kubota, Tetsuya; Hirama, Toshiyasu; Verbeek, Walter; Chih, Doris Y.; Chumakov, Alexey M.; Taguchi, Hirokuni; Koeffler, H. Phillip

doi:10.1016/s0145-2126(01)00065-0

Cited by 5 publications

(11 citation statements)

References 37 publications

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“…Our previous experiments (Kubota et al, 2000, also unpublished observations) indicated that both mXCP1 and mXCP2 were strongly expressed in peritoneal exudates derived from wild-type mice following TG injection and were completely absent from exudates of similarly treated C/EBP-e À/À mice. To confirm that mXCP1 expression is indeed regulated by C/EBP-e, we have analysed mRNA derived from the spleen, bone marrow, colon and skin of wild-type and C/EBP-e knockout mice following TG administration using Northern blotting and hybridization with mXCP1-and mXCP2-specific probes (Figure 3b).…”

Section: Xcp1 Is a Strictly C/ebp-e-dependent Genementioning

confidence: 71%

“…We have previously used a PCR-driven RDA assay to detect genes that are directly regulated by C/EBP-e (Kubota et al, 2000). Briefly, cDNA derived from the myeloid cells (peritoneal exudate following intraperitoneal (i.p.)…”

Section: Resultsmentioning

confidence: 99%

“…Since the majority of genes identified in the course of our original RDA subtraction experiments were either chemokines or chemokine receptors (Kubota et al, 2000), perhaps mXCP1 also has a role in cell migration, activation and chemotaxis. To test this hypothesis, we used a Boyden cell migration chamber to compare cell motility either in the presence or absence of XCP1 in the media.…”

Section: Identification Of Xcp1 Activitymentioning

confidence: 99%

“…Positions of GST-defensin fusion protein (GST-DEF) and XCP1 are indicated by arrows Williams et al, 1991). In our previous study, we used PCR-based RDA analysis of peritoneal cells from C/EBP-e knockout mice to identify genes that are specifically absent in C/EBP-e À/À mice and are likely targets of C/EBP-e regulation (Kubota et al, 2000). Approximately 10% of identified clones were derived from a novel gene that we named mXCP1 (mouse ten-cysteine containing protein 1).…”

Section: Identification Of Mxcp1 Genementioning

confidence: 99%

“…The lack of secondary granule proteins in granulocytes from these mice results in the impairment of responses to inflammatory signals (Masera et al, 1996;Lekstrom-Himes et al, 1999). We used C/EBP-e knockout mice to identify myelomonocytic genes regulated by C/EBP-e (Kubota et al, 2000). Polymerase chain reaction (PCR)-based subtractive hybridization method RDA (representational difference analysis) was used to find genes that are not expressed in neutrophils and macrophages of C/EBP-e knockout mice.…”

Section: Introductionmentioning

confidence: 99%

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Identification of murine and human XCP1 genes as C/EBP-ε-dependent members of FIZZ/Resistin gene family

et al. 2004

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Section: Xcp1 Is a Strictly C/ebp-e-dependent Genementioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Xcp1 Activitymentioning

confidence: 99%

Section: Identification Of Mxcp1 Genementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of murine and human XCP1 genes as C/EBP-ε-dependent members of FIZZ/Resistin gene family

et al. 2004

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C/EBPε participates in all-trans retinoic acid induction of PI3Kγ in U937 cells via an intronic matrix attachment region sequence

Cai

Chen

et al. 2010

Mol Biol Rep

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ATRA (all-trans retinoic acid) regulates gene expression by binding as a ligand to its specific receptors like C/EBPε which is directly induced. In the U937 cell line, PI3Kγ is selectively induced over other PI3Ks by ATRA, although the mechanism is still unclear. Here, we show that C/EBPε and PI3Kγ are induced in U937 cells by ATRA both in levels of mRNA and protein. Reporter gene assay revealed that C/EBPε is able to interact with a previously identified 2 kb MAR (matrix attachment region) sequence in the last intron of PI3Kγ gene, and increases its linked heterogeneous reporter gene expression. ChIP assay showed that induction of endogenous PI3Kγ is at least partially caused by enhanced, direct C/EBPε binding to a 15 bp sequence at nucleotides 1428-1442 within this MAR sequence, and EMSA analysis confirmed this binding in vitro. The results above collectively show that C/EBPε participates in ATRA induction of PI3Kγ.

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C/EBPδ expression in a BCR-ABL-positive cell line induces growth arrest and myeloid differentiation

et al. 2005

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CCAAT/enhancer-binding proteins (C/EBPs) are a family of highly conserved transcription factors that have important roles in normal myelopoiesis as well as associated with myeloid disorders. The chronic myelogenous leukemia (CML) cell lines, KCL22 and K562, express exceptionally low levels of endogenous C/EBPs and provide a good model to test the effects of C/EBPs on myeloid differentiation. To explore the possibility that C/ EBPd can promote differentiation in BCR-ABL-positive cells, we generated stable KCL22 and K562 clones that expressed an inducible C/EBPd gene. C/EBPd expression resulted in G0/G1 proliferative arrest and a moderate increase in apoptosis of the KCL22 and the K562 cells. Within 4 days of inducing expression of C/EBPd, myeloid differentiation of the CML blast cells occurred as shown by morphologic changes and induction of secondary granule-specific genes. We also showed that during granulocytic differentiation of KCL22 cells, the C/EBPd protein was detected in immunocomplexes with both Rb and E2F1. Furthermore, expression of C/EBPd was associated with downregulation of c-Myc and cyclin E and upregulation of the cyclin-dependent kinase inhibitor p27Kip1 in both the KCL22 and K562 cell lines. These results show that expression of C/EBPd in BCR-ABLpositive leukemic cells in blast crisis is sufficient for neutrophil differentiation and point to the therapeutic potential of ectopic induction of C/EBPd in the acute phase of CML.

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DNase I hypersensitivity analysis of the human CCAAT enhancer binding protein ε (C/EBPε) gene

Cited by 5 publications

References 37 publications

Identification of murine and human XCP1 genes as C/EBP-ε-dependent members of FIZZ/Resistin gene family

Identification of murine and human XCP1 genes as C/EBP-ε-dependent members of FIZZ/Resistin gene family

C/EBPε participates in all-trans retinoic acid induction of PI3Kγ in U937 cells via an intronic matrix attachment region sequence

C/EBPδ expression in a BCR-ABL-positive cell line induces growth arrest and myeloid differentiation

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