DNase-I functionalization of ciprofloxacin-loaded chitosan nanoparticles overcomes the biofilm-mediated resistance of Pseudomonas aeruginosa

Patel, Krishna Kumar; Agrawal, Ashish Kumar; Anjum, Md Meraj; Tripathi, Muktanand; Pandey, Nidhi; Bhattacharya, Subhamoy; Tilak, Ragini; Singh, Sanjay

doi:10.1007/s13204-019-01129-8

Cited by 39 publications

(26 citation statements)

References 43 publications

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“…The use of this non-biocidal molecule, fully functional in its natural lipid-rich environment both in vitro and in vivo, may alter the biofilm structure by degrading eDNA and lead to the release of planktonic P. acnes susceptible to antibiotics. The usage of nucleases such as the recombinant human DNase is an effective and safe approach already described for Pseudomonas aeruginosa biofilm treatment in cystic fibrosis infection (Patel et al, 2020;Yang and Montgomery, 2021). Using enzymes that can weaken or destroy the biofilm structure can represent a new strategy to combat bacterial biofilms in acne vulgaris.…”

Section: Discussionmentioning

confidence: 99%

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Bronnec

Eilers

Jahns

et al. 2022

Front. Cell. Infect. Microbiol.

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Acne vulgaris is the most common dermatological disorder worldwide affecting more than 80% of adolescents and young adults with a global prevalence of 231 million cases in 2019. The involvement of the skin microbiome disbalance in the pathophysiology of acne is recognized, especially regarding the relative abundance and diversity of Propionibacterium acnes a well-known dominant human skin commensal. Biofilms, where bacteria are embedded into a protective polymeric extracellular matrix, are the most prevalent life style for microorganisms. P. acnes and its biofilm-forming ability is believed to be a contributing factor in the development of acne vulgaris, the persistence of the opportunistic pathogen and antibiotic therapy failures. Degradation of the extracellular matrix is one of the strategies used by bacteria to disperse the biofilm of competitors. In this study, we report the identification of an endogenous extracellular nuclease, BmdE, secreted by Propionibacterium granulosum able to degrade P. acnes biofilm both in vivo and in vitro. This, to our knowledge, may represent a novel competitive mechanism between two closely related species in the skin. Antibiotics targeting P. acnes have been the mainstay in acne treatment. Extensive and long-term use of antibiotics has led to the selection and spread of resistant bacteria. The extracellular DNase BmdE may represent a new bio-therapeutical strategy to combat P. acnes biofilm in acne vulgaris.

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Section: Discussionmentioning

confidence: 99%

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Bronnec

Eilers

Jahns

et al. 2022

Front. Cell. Infect. Microbiol.

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“…DNase I is most frequently used as a dispersant coating for nanoparticles. Particularly chitosan nanoparticles are suitable for DNase I coating due to their abundant amino groups that can be covalently coupled to DNase I [103]. In addition to DNase coating, DNase I coated chitosan nanoparticles have been loaded with ciprofloxacin to disperse P. aeruginosa biofilms by degrading eDNA and kill detached bacteria [103].…”

Section: Nanoparticles Coated With Dispersant Moleculesmentioning

confidence: 99%

“…Particularly chitosan nanoparticles are suitable for DNase I coating due to their abundant amino groups that can be covalently coupled to DNase I [103]. In addition to DNase coating, DNase I coated chitosan nanoparticles have been loaded with ciprofloxacin to disperse P. aeruginosa biofilms by degrading eDNA and kill detached bacteria [103]. The simultaneous release of ciprofloxacin counters the risk of causing acute sepsis due to the detachment of infectious pathogens from a biofilm in the blood circulation.…”

Section: Nanoparticles Coated With Dispersant Moleculesmentioning

confidence: 99%

Recent advances and future challenges in the use of nanoparticles for the dispersal of infectious biofilms

Tian

Mei

Ren

et al. 2021

Journal of Materials Science & Technology

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“…The DNase Test Agar plate also contains Methyl Green as an indicator. Deoxyribonuclease breaks DNA in the medium into smaller fragments which can no longer bind to Methyl Green, and thus a clear halo appears around the colonies of DNase-producing bacteria (Patel et al, 2019). This test is used to differentiate between DNase producers like Staphylococcus aureus, Serratia etc.…”

Section: Dnasementioning

confidence: 99%

Bacterial enzymes as diagnostic tools for various human pathogens

Vachher¹,

Sen²,

Burman³

2021

JSR

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Rapid and accurate diagnosis of diseases is essential for early therapeutic intervention and efficacious treatment. Infectious diseases can be devastating with very high mortality and morbidity rates. These are caused by diverse organisms which replicate in their hosts. Enzymes are proteinaceous biocatalysts in nature, except ribozymes, which catalyze diverse reactions indispensable for life. Enzymes have numerous applications owing to their unique features like high substrate specificity, immense catalytic efficiency, diverse pH and temperature optima and the ability to be regulated. Enzymes play an essential and inimitable role in clinical diagnosis and management. Detection of enzymes produced by certain pathogens can help in apt and timely diagnosis of the infectious agent. This can favorably result in a positive outcome and successful therapeutic intervention and thus save lives. In this review, we attempt to summarize microbial enzymes including urease, coagulase, DNase, gelatinase, catalase, oxidase, βlactamase, tryptophanase and lipase, which are being used in the clinical diagnosis of diseases caused by pathogens and thus they positively influence the disease outcome. Owing to the various novel characteristics possessed by these enzymes, they have become a method of choice for rapid, accurate, economical and simple diagnostics.

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DNase-I functionalization of ciprofloxacin-loaded chitosan nanoparticles overcomes the biofilm-mediated resistance of Pseudomonas aeruginosa

Cited by 39 publications

References 43 publications

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism

Recent advances and future challenges in the use of nanoparticles for the dispersal of infectious biofilms

Bacterial enzymes as diagnostic tools for various human pathogens

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