DnaQ mediates directional spacer acquisition in the CRISPR-Cas system by a time-dependent mechanism

Tang, Dongmei; Jia, Tingting; Luo, Yongbo; Mou, Biqin; Cheng, Jie; Qi, Shiqian; Yao, Shaohua; Su, Zhaoming; Yu, Yamei; Chen, Qiang

doi:10.1016/j.xinn.2023.100495

Cited by 1 publication

(1 citation statement)

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“…Furthermore, the gene editing technologies can also be introduced into SPR-based detection of nucleic acids. For example, a "gene scissors" of clustered regularly interspaced short palindromic repeat (CRISPR) platform 204,205 has been proposed to combine with SPR. Recently, a CRISPR-empowered SPR diagnostic strategy was established to fulfill accurate detection of ~ 5 fM unamplified DNA samples.…”

Section: Surface Plasmon Resonance (Spr)-based Dna Nanostructuresmentioning

confidence: 99%

Advances of fluorescent DNA nanostructures in biomedical applications

Shen,

Cao,

Xing

et al. 2024

TIMS

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With the rapid development of DNA nanotechnology, the emergence of fluorescent DNA nanostructures (FDNs) has enlarged the biological applications. FDNs have great advantages of precise localization and real-time tracing in bioimaging. In this review, the recent biomedical developments of FDNs have been reviewed, including the design of FDNs, and the corresponding applications on biomarker sensing, bioimaging, cancer diagnosis and therapy. Firstly, the development of DNA nanostructures and the corresponding DNA-based nanomaterials were briefly introduced. Simultaneously, to make a better demonstration, the background and theory of the fluorescence detections were briefly introduced. Thereafter, the synthetic strategies of DNA nanostructure were summarized and classified, which facilitated the multiple functionalizations for sensing and bioimaging. Subsequently, the biomedical applications of FDNs are comprehensively summarized based on different detection strategies, including fluorescence resonance energy transfer (FRET), aggregation-induced emission (AIE), near-infrared (NIR)-photoactivation, nucleic acid amplification (NAT), small fluorescent dyes loading, and surface plasmon resonance (SPR) technologies. Finally, an insight into the challenges and future perspectives is provided. As reviewed, FDNs are important tools in precision medicine, showing great potential in both in vivo and in vitro cancer diagnosis and treatments. Undoubtedly, FDN-based technology is a promising strategy for constructing versatile nanodevices in biological applications and will excel in human healthcare.

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Section: Surface Plasmon Resonance (Spr)-based Dna Nanostructuresmentioning

confidence: 99%