DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1 expression <i>via</i> inhibition of miR-432-5p

Lei, Ting; Li, Si-Qiao; Mai, Erhui; Ding, Feihu; Niu, Ben

doi:10.3748/wjg.v26.i15.1745

Cited by 21 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have revealed the antitumour activity of miR-432-5p, with Zhang et al reporting that miR-432-5p can mediate cisplatin resistance in NSCLC through its ceRNA function towards the long noncoding RNA MSTRG.51053.2 [ 37 ]. In addition, Xu et al observed that miR-432-5p can could suppress pancreatic carcinoma progression by downregulating PPME1 [ 38 ]. To further elucidate the underlying mechanism of miR-432-5p, we conducted bioinformatic, RT-qPCR and western blot analyses, with the results identifying ADAR1 as the most likely target of miR-432-5p.…”

Section: Discussionmentioning

confidence: 99%

CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing

et al. 2021

View full text Add to dashboard Cite

Background A growing number of studies have focused on investigating circRNAs as crucial regulators in the progression of multiple cancer types. Nevertheless, the biological effects and underlying mechanisms of circRNAs in pancreatic ductal adenocarcinoma (PDAC) remain unclear. Methods Differentially expressed circRNAs between cancerous tissue and adjacent normal tissues were identified by RNA sequencing in PDAC. Subsequently, in vitro and in vivo functional experiments were performed to investigate the functional roles of circNEIL3 in PDAC tumour growth and metastasis. Furthermore, RNA pull-down, dual-luciferase reporter assays, RNA immunoprecipitation (RIP) assays, fluorescent in situ hybridization (FISH) and Sanger sequencing assays were performed to examine the circular interaction among circNEIL3, miR-432-5p and adenosine deaminases acting on RNA 1 (ADAR1). Results CircNEIL3 was upregulated in PDAC and promoted the progression of PDAC cells both in vitro and in vivo. Mechanistically, circNEIL3 was shown to regulate the expression of ADAR1 by sponging miR-432-5p to induce RNA editing of glioma-associated oncogene 1 (GLI1), ultimately influencing cell cycle progression and promoting epithelial-to-mesenchymal transition (EMT) in PDAC cells. Moreover, we discovered that the circNEIL3/miR-432-5p/ADAR1 axis was correlated with the PDAC clinical stage and overall survival of PDAC patients, while ADAR1 may reduce the biogenesis of circNEIL3. Conclusions Our findings reveal that circNEIL3 facilitates the proliferation and metastasis of PDAC through the circNEIL3/miR-432-5p/ADAR1/GLI1/cell cycle and EMT axis and that its expression is regulated by ADAR1 through a negative feedback loop. Therefore, circNEIL3 may serve as a prognostic marker and a therapeutic target for PDAC.

show abstract

Section: Discussionmentioning

confidence: 99%

CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Xu et al demonstrated that high miR-432-5p expression repressed pancreatic carcinoma progression by targeting PPME1. 27 Liu et al discovered that decreased miR-432-5p expression may facilitate the proliferation and migration of breast cancer cells by modulating E2F3 expression. 18 In addition, Liu et al demonstrated that miR-432-5p was also involved in the proliferation and metastasis of hepatocellular carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

CircRNA DUSP16 Knockdown Suppresses Colorectal Cancer Progression by Regulating the miR-432-5p/E2F6 Axis

Wang¹,

Yang²

2021

CMAR

View full text Add to dashboard Cite

“…ADNAH17-AS1 is a kind of lncRNA that has been identi ed latest, while few reports revealed its potential effects. In pancreatic carcinoma, DNAH17-AS1 was found to be overexpressed and promote pancreatic carcinoma cells' proliferation and migration by upregulating PPME1 expression via sponging miRNA-432-5p, indicating that DNAH17-AS1 acted as tumor promotor in this tumor 16 . In this study, we also found the distinct upregulation of DNAH17-AS1 in NSCLC, which was consistent with its expressing trend in pancreatic carcinoma.…”

Section: Discussionmentioning

confidence: 97%

“…Long non-coding RNA DNAH17 antisense RNA 1 (DNAH17-AS1) can be found in the 17q25.3, and is a kind of lncRNA related to tumors. Studies reported its high expression and the ability to promote tumor progression in pancreatic carcinoma and colorectal cancer 16,17 . Nevertheless, its expression pattern as well as potential function in other tumors was still not well understood.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA DNAH17-AS1 promotes tumorigenesis and metastasis of non-small cell lung cancer via regulating miR-877–5p/CCNA2 pathway

Mao

Jing

2020

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Background: A growing number of studies have revealed that long noncoding RNAs (lncRNAs) can function as important oncogenes or tumor suppressors. This study aimed to investigate the regulatory role of lncRNA DNAH17 antisense RNA 1 (DNAH17-AS1) on non-small cell lung cancer (NSCLC) and the underlying molecular mechanisms. Methods: RT-PCR was used to examine the expression of DNAH17-AS1, miR-877-5p and CCNA2 in NSCLC specimens and cell lines. The diagnostic and prognostic values of DNAH17-AS1 expression in NSCLC patients were statistically analyzed. We also evaluated the effects of DNAH17-AS1 on the proliferation, migration, invasion and apoptosis of H1299 and 95D cells. Bioinformatic analysis and luciferase reporter assays were carried to con rm the molecular binding. Results: The expression of DNAH17-AS1 and CCNA2 mRNA was distinctly upregulated in NSCLC specimens and cell lines, while miR-877-5p expression was signi cantly decreased. DNAH17-AS1 could be used to distinguish NSCLC specimens from adjacent non-tumor tissues. Clinical assays revealed that high DNAH17-AS1 was associated with TNM stage, distant metastasis and shorter overall survival and disease-free survival. Functional assays indicated that knockdown of DNAH17-AS1 suppressed the proliferation, migration and invasion of H1299 and 95D cells, and promoted apoptosis. Mechanically, DNAH17-AS1 served as competing endogenous RNA (ceRNA) for miR-877-5p to positively recover CCNA2. Conclusion: We identi ed a novel NSCLC-related lncRNA, DNAH17-AS1 which may exert an oncogenic function via serving as a sponge for miR-877-5p to upregulate CCNA2. Our study presents novel insights into NSCLC progression and provided a prospective therapeutic target for NSCLC.

show abstract

DNAH17-AS1 promotes pancreatic carcinoma by increasing PPME1 expression via inhibition of miR-432-5p

Cited by 21 publications

References 29 publications

CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing

CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing

CircRNA DUSP16 Knockdown Suppresses Colorectal Cancer Progression by Regulating the miR-432-5p/E2F6 Axis

Long noncoding RNA DNAH17-AS1 promotes tumorigenesis and metastasis of non-small cell lung cancer via regulating miR-877–5p/CCNA2 pathway

Contact Info

Product

Resources

About