DNA variation in the gene ELTD1 is associated with tick burden in cattle

Abstract

Ticks and tick-born diseases are major constraints on cattle production in tropical and subtropical regions in the world. Previously, we identified single nucleotide polymorphisms (SNPs) associated with tick resistance on bovine chromosome 3 at approximately 70 Mb. In this study, we genotyped a dairy (n = 1133) and a beef (n = 774) sample to confirm the association of the intronic SNP rs29019303 and its gene (ELTD1) with tick burden. We genotyped 18 additional SNPs in a region of 181 kb and found that rs29019303 was significantly (P < 0.05) associated with tick burden in both samples with the same favourable allele. A second SNP in this same genomic region was also significantly associated with tick burden in each sample. The associations using haplotypes were stronger than for single markers, including a haplotype of nine tag SNPs that was highly significantly (P = 0.0008) associated with tick counts in the dairy animals. This haplotype and two others were significant after Bonferroni correction for multiple testing. The estimated size of the effects was close to 0.9% of the residual variance in both samples tested.

Cattle Tick Rhipicephalus microplus-Host Interface: A Review of Resistant and Susceptible Host Responses

“…The ELTD1 gene (EGF, latrophilin, and seven transmembrane domain containing 1) was identified from GWAS in dairy and beef cattle (Prayaga et al, 2009 ; Turner et al, 2010 ). Its association with the host resistance phenotype was confirmed but its effect was limited to <1% of the total phenotypic variation in the trait (Porto Neto et al, 2011a ). Similarly, haplotypes that included the ITGA11 gene (integrin alpha 11) were significantly associated with tick burden and explained about 1.5% of the variation in the trait.…”

“…Numerous studies have attempted to identify genetic markers for host resistance to tick infestation and they are summarized and discussed by Porto Neto et al ( 2011b ) and Mapholi et al ( 2014 ). Approaches have included immunological methods (Stear et al, 1984 , 1989 , 1990 ); protein-based analyses (Ashton et al, 1968 ; Carvalho et al, 2008 ); candidate gene sequence or genotype (Acosta-Rodriguez et al, 2005 ; Martinez et al, 2006 ; Untalan et al, 2007 ); genomic detection of quantitative trait loci (QTL) using SNPs or microsatellites, with or without fine mapping (Barendse, 2007 ; Gasparin et al, 2007 ; Regitano et al, 2008 ; Prayaga et al, 2009 ; Machado et al, 2010 ; Porto Neto et al, 2010a , 2011a ; Turner et al, 2010 ; Cardoso et al, 2015 ; Mapholi et al, 2016 ; Sollero et al, 2017 ). There is one example of a meta-analysis of genomic association with transcriptome in tick infestation (Porto Neto et al, 2010b ).…”

Signatures of Selection in CompositeVrindavaniCattle of India

“…TNNI3K is a cardiac troponin interacting kinase, associated with udder depth (Kramer et al, 2014), inflammation mechanisms (Wiltshire et al, 2011) and lameness in Holstein–Friesian cattle (Sánchez-Molano et al, 2019). The ADGRL4/ELTD1 gene is associated with milk fat yield (Li et al, 2010) and tick resistance (Porto Neto et al, 2011) in dairy cattle, average daily gain in pigs (Hong et al, 2017), and genetic predisposition for obesity in both humans and pigs (Lee et al, 2011). Another gene DDX1 on BTA11 was reported to be involved in bovine mammary involution in environmental stress conditions (Dado-Senn et al, 2018).…”

Epidermal growth factor, latrophilin, and seven transmembrane domain-containing protein 1 marker, a novel angiogenesis marker

“… 22 Upregulation of the ELTD1 gene was observed in studies describing genomic adaption to high altitudes in the Andean population, 23 cannabis use disorders, 24 genetic predisposition for obesity in both humans and pigs, 25 and even resistance to therapy in cattle afflicted by ticks. 26 …”