DNA Variation at the Invertase Locus invGE/GF Is Associated With Tuber Quality Traits in Populations of Potato Breeding Clones

Li, Li; Strahwald, Josef; Hofferbert, Hans-Reinhard; Lübeck, Jens; Tacke, Eckhard; Junghans, H.; Wunder, Jörg; Gebhardt, Christiane

doi:10.1534/genetics.104.040006

Cited by 80 publications

(88 citation statements)

References 44 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strong correlation between reducing sugar content and chipping colour has previously been demonstrated (Scheffler et al 1992). QTLs for chipping colour in our study confirm that major QTL are present in regions on chromosomes 3, 5, 8, 9 and 10 (Douches and Freyre 1994;Menendez et al 2002;Bradshaw et al 2008;Li et al 2005), although strict comparisons with published data cannot be made due to the lack of shared markers and map resolution. For the chipping QTLs on chromosomes 3 we confirm the co-localisation with starch phosphorylase StPho1a, but the invertase locus PAIN-1 and SSS-1 have been implicated as well (Li et al 2008).…”

Section: Genetic Variation For Starch Propertiessupporting

confidence: 81%

A limited set of starch related genes explain several interrelated traits in potato

et al. 2012

View full text Add to dashboard Cite

To understand the molecular basis of potato starch related traits and the underlying starch biosynthesis and degradation, a Quantitative Trait Locus (QTL) analysis in combination with a candidate gene approach was performed. The diploid mapping population C 9 E, consisting of 249 individuals, was assayed over two consecutive years, for chipping colour, cold induced sweetening, starch content, starch granule size, starch gelling temperature, starch enthalpy, amylose content and degree of starch phosphorylation. QTLs were observed for all traits, except enthalpy on eight out of the twelve potato chromosomes. Several QTLs were found to be consistent over 2 years. Clustering of co-localizing QTLs was observed on some chromosomes, indicating common genetic factors for the different traits. On chromosome 2, Soluble Starch Synthase 2 mapped on the same position as QTLs for starch phosphorylation, starch gelling temperature and amylose content. a-glucan, water dikinase co-localizes on chromosome 5 together with QTLs for starch phosphorylation and cold induced sweetening. Furthermore, the genes coding for two phosphorylases (StPho1a and StPho2) coincide with QTLs for starch gelling temperature, chipping colour and starch granule size on chromosome 2 and a QTL for starch phosphorylation on chromosome 9, respectively. The results suggest allelic variation acting on the genetics of the different traits.

show abstract

Section: Genetic Variation For Starch Propertiessupporting

confidence: 81%

A limited set of starch related genes explain several interrelated traits in potato

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The observed phenotypic and genotypic variability not only results from recombination, but also from a larger number, when compared with diploids, of chromosomal haplotype combinations that are possible in 4x by 4x crosses with tetrasomic inheritance. This might be one reason for the large genetic distances of similar size, which usually separate tetraploid individuals despite their relatedness by pedigree, and thereby the lack of population structure (Li et al 2005). Another reason for lack of population structure might be an underlying uniformity of the genetic material due to its selective adaptation for general agronomic performance.…”

Section: Discussionmentioning

confidence: 99%

Using SNP markers to dissect linkage disequilibrium at a major quantitative trait locus for resistance to the potato cyst nematode Globodera pallida on potato chromosome V

et al. 2008

Self Cite

View full text Add to dashboard Cite

The damage caused by the parasitic root cyst nematode Globodera pallida is a major yield-limiting factor in potato cultivation . Breeding for resistance is facilitated by the PCR-based marker 'HC', which is diagnostic for an allele conferring high resistance against G. pallida pathotype Pa2/3 that has been introgressed from the wild potato species Solanum vernei into the Solanum tuberosum tetraploid breeding pool. The major quantitative trait locus (QTL) controlling this nematode resistance maps on potato chromosome V in a hot spot for resistance to various pathogens including nematodes and the oomycete Phytophthora infestans. An unstructured sample of 79 tetraploid, highly heterozygous varieties and breeding clones was selected based on presence (41 genotypes) or absence (38 genotypes) of the HC marker. Testing the clones for resistance to G. pallida conWrmed the diagnostic power of the HC marker. The 79 individuals were genotyped for 100 single nucleotide polymorphisms (SNPs) at 10 loci distributed over 38 cM on chromosome V. Forty-Wve SNPs at six loci spanning 2 cM in the interval between markers GP21-GP179 were associated with resistance to G. pallida. Based on linkage disequilibrium (LD) between SNP markers, six LD groups comprising between 2 and 18 SNPs were identiWed. The LD groups indicated the existence of multiple alleles at a single resistance locus or at several, physically linked resistance loci. LD group C comprising 18 SNPs corresponded to the 'HC' marker. LD group E included 16 SNPs and showed an association peak, which positioned one nematode resistance locus physically close to the R1 gene family.Communicated by J. E. Bradshaw. Electronic supplementary materialThe online version of this article

show abstract

“…In tomato fruits, a QTL for total soluble solids turned out to be a CW-Inv (18). In potato, a cold-sweetening QTL was due to an Inv gene, that, based on homology, was classified as cell wall-bound (20).…”

Section: A Role For Vacuolar or Cell Wall Invertase In Controlling Elmentioning

confidence: 99%

“…QTLs for Inv have been studied in tomato (18,19) and potato (20), where genetic colocation or heterologous complementation indicate that Inv polymorphisms are responsible for sugar content͞ composition.…”

mentioning

confidence: 99%

Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis

Сергеева

Keurentjes

Bentsink

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

177

128

View full text Add to dashboard Cite

The possible role of the sucrose-splitting enzymes sucrose synthase and invertase in elongating roots and hypocotyls of Arabidopsis was tested by using a combination of histochemical methods and quantitative trait locus (QTL) analysis. Lengths of roots and hypocotyls correlated better with invertase activities than with sucrose synthase activities. The highest correlations were observed with activities in the elongating zones of roots. The genetic basis of these correlations was studied by using QTL analysis. Several loci, affecting invertase activity, colocated with loci that had an effect on root or hypocotyl length. Further fine mapping of a major locus for root length, but not for hypocotyl length (top chromosome 1), consistently showed colocation with the locus for invertase activity containing a gene coding for a vacuolar invertase. The analysis of a functional knockout line confirmed the role of this invertase in root elongation, whereas other invertase genes might play a role in hypocotyl elongation. Thus, we show the power of QTL analysis, combined for morphological and biochemical traits, followed by fine-mapping and mutant analysis, in unraveling the function of genes and their role in growth and development.Arabidopsis natural variation ͉ sucrose synthase ͉ hypocotyls T otal plant yield depends on the acquisition of raw material, i.e., photosynthesis and mineral (plus water) uptake, and on the ability of the plant to cope with stress. However, the economic yield of a crop is to a large extent also determined by the partitioning of dry matter over the harvestable and nonharvestable parts of the plant. The molecular and physiological basis of the regulation of assimilate partitioning in plants is still poorly understood. In terms of biomass, the most important components in assimilate partitioning and in total yield are carbohydrates. There is increasing evidence that a limited number of key enzymes, involved in primary (carbohydrate) metabolism, might be pivotal in this process (1).Functionally, a plant can be divided into sources (the sites of assimilate production) and sinks (the sites of use and͞or storage). Sinks can either be rapidly growing, expanding organs, such as elongating stems and roots, or storage sinks accumulating reserves, such as fruits, seeds, or tubers (2).In most plant species, carbon is transported from source to sink in the form of the disaccharide sucrose. Upon arrival in the sink, sucrose has to be hydrolyzed. In plants, two pathways are available for sucrose cleaving: via invertase (Inv), yielding glucose and fructose, and via sucrose synthase (Susy), yielding fructose and UDP-glucose. In several cases, it has been suggested that sink strength might depend on the activities of these sucrose-splitting enzymes. There is increasing evidence that in storage sinks, the predominant pathway is via Susy, whereas in growing sinks, the Inv route is most important. In potatoes, the elongating rhizomes (stolons) exhibit high Inv activity, whereas a switch toward Susycatalyzed sucrose bre...

show abstract

DNA Variation at the Invertase Locus invGE/GF Is Associated With Tuber Quality Traits in Populations of Potato Breeding Clones

Cited by 80 publications

References 44 publications

A limited set of starch related genes explain several interrelated traits in potato

A limited set of starch related genes explain several interrelated traits in potato

Using SNP markers to dissect linkage disequilibrium at a major quantitative trait locus for resistance to the potato cyst nematode Globodera pallida on potato chromosome V

Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis

Contact Info

Product

Resources

About