DNA Vaccines

Encke, Jens; Putlitz, Jasper zu; Wands, Jack R.

doi:10.1159/000024971

Cited by 35 publications

(20 citation statements)

References 52 publications

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“…Another important advantage of DNA vaccines is their therapeutic potential in ongoing chronic viral infections. DNA vaccines are very promising tools for an effective induction of a protective immune response against viral infections (HBV, HCV, HIV) and parasitic infections (malaria, leishmaniasis) [26,40,63].…”

Section: The Advantages Of Dna Vaccinationmentioning

confidence: 99%

“…The gene-gun or biolistic system uses compressed helium to propel micrometer-sized colloidal gold particles coated with precipitated plasmid DNA (DNA-coated microparticles) directly into the epidermal cells. In mice, intraepidermal gene-gun DNA inoculation generates a prominent Th2 response with interleukin (IL)-4 production and an excess of the IgG1 isotype [26,36,40]. The approximately log 10 of the DNA dose may explain the dominance of the Th2 response because low plasmid doses result in a low CpG motif moiety, which is important for a Th1 response elicitation [62,87].…”

Section: The Enhancement Of Efficacy Of Dna Vaccinationmentioning

confidence: 99%

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DNA vaccines: are they still just a powerful tool for the future?

Bĕláková

Horynová

Křupka

et al. 2007

Arch. Immunol. Ther. Exp.

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Vaccination is historically one of the most successful strategies for the prevention of infectious diseases. For safety reasons, modern vaccinology tends toward the usage of inactivated or attenuated microorganisms and uses predominantly subunit vaccines. The antigens need to be clearly defined, pure, stable, appropriately composed, and properly presented to the immune system of the host. Differing ratios of various proportions between specific CD4+ and CD8+ T cell responses are essential for conferring the required protection in the case of individual vaccines. To stimulate both CD4+ and CD8+ T cells, the antigens must be processed and presented to both antigen-presentation pathways, MHC I and MHC II. Protein antigens delivered by vaccination are processed as extracellular antigens. However, extracellularly delivered antigen can be directed towards intracellular presentation pathways in conjugation with molecules involved in antigen cross-presentation, e.g. heat shock proteins, or by genomic-DNA vaccination. In this overview, current knowledge of the host immune response to DNA vaccines is summarized in the introduction. The subsequent sections discuss techniques for enhancing DNA vaccine efficacy, such as DNA delivery to specific tissues, delivery of DNA to the cell cytoplasm or nucleus, and enhancement of the immune response using molecular adjuvants. Finally, the prospects of DNA vaccination and ongoing clinical trials with various DNA vaccines are discussed.

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Section: The Advantages Of Dna Vaccinationmentioning

confidence: 99%

Section: The Enhancement Of Efficacy Of Dna Vaccinationmentioning

confidence: 99%

DNA vaccines: are they still just a powerful tool for the future?

Bĕláková

Horynová

Křupka

et al. 2007

Arch. Immunol. Ther. Exp.

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“…Peptides were proposed by computer simulation (University of Wisconsin Genetics Computer Group (UWGCG), peptide structure program) and prepared by EMC microcollections (Tübingen, Germany) with the following sequences: YQVRNSSGLYHVTNDCPNSS (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), PGCVPCVREGNAS RCWVAVT (33-53), REGNASRCWVAVTPTVATRDGKL and PRRHWTTQDCNCSIYPG (104-120). Establishment and characterization of the stable transfected cell line expressing HCV core protein (SP2-19) and the control cell line SP2-0 have been described previously [34].…”

Section: Antigens and Cell Linesmentioning

confidence: 99%

Prophylactic and therapeutic vaccination with dendritic cells against hepatitis C virus infection

Encke

Findeklee²,

Geib³

et al. 2005

Clinical and Experimental Immunology

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“…In animal models direct intramuscular or intradermal injection of plasmid DNA generates potent cell-mediated and humoral immune responses against a variety of pathogens, such as HIV [2] , hepatitis B virus [3] and mycobacteria [4] . An important feature of DNAbased immunization is the induction of cytotoxic T lymphocytes (CTLs) that recognize and lyse virus-infected cells, thereby limiting viral spread.…”

Section: Introductionmentioning

confidence: 99%

Intramuscular Immunization with a Plasmid DNA Vaccine Encoding prM-E Protein from Japanese Encephalitis Virus: Enhanced Immunogenicity by Co-Administration of GM-CSF Gene and Genetic Fusions of prM-E Protein and GM-CSF

Zhai

Zhou

et al. 2009

Intervirology

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Objective: To investigate the immune responses elicited by pJME with or without various forms of granulocyte-macrophage colony-stimulating factor (GM-CSF) gene. Methods: The changes of the T lymphocyte subsets and the levels of Th cell intracellular cytokines IFN-γ and IL-4 were evaluated by flow cytometric analysis. The cytotoxic T lymphocyte kill activity was assessed by lactate dehydrogenase activity release test. An 80% plaque reduction neutralization test was performed to titrate the neutralization antibody before and after viral challenge. Results: We demonstrated that simultaneous administration of pJME plus plasmid-ecoded GM-CSF (pGM-CSF) activated Th1 immune responses similar to those found by injecting pGM-CSF i.m. into mice 3 days before pJME vaccination, and enhancement of Th2 immunity predominated when the pGM-CSF was injected 3 days after pJME vaccination. Furthermore, the immunization with DNA vaccine encoding precursor membrane envelope/GM-CSF fusion protein was more effective in generating immune responses than that induced by immunization with pJME alone or in combination with pGM-CSF. Conclusions: These observations support the potential of GM-CSF DNA adjuvant for the Th1/Th2 balance and the enhancement of immune responses by showing that the timing of the administration of pGM-CSF and the application of different forms of GM-CSF gene influence the outcome of the resultant immune responses.

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DNA Vaccines

Cited by 35 publications

References 52 publications

DNA vaccines: are they still just a powerful tool for the future?

DNA vaccines: are they still just a powerful tool for the future?

Prophylactic and therapeutic vaccination with dendritic cells against hepatitis C virus infection

Intramuscular Immunization with a Plasmid DNA Vaccine Encoding prM-E Protein from Japanese Encephalitis Virus: Enhanced Immunogenicity by Co-Administration of GM-CSF Gene and Genetic Fusions of prM-E Protein and GM-CSF

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