DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica

Dai, Yang; Zhu, Yinchang; Harn, Donald A.; Wang, Xiaoting; Tang, Jianxia; Song, Zhao; Lu, Fei; Guan, Xiaohong

doi:10.1128/cvi.00231-09

Cited by 30 publications

(20 citation statements)

References 35 publications

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“…Thus far, these candidates have not induced adverse reactions, but have induced immune responses. Other candidates that are in preclinical trials at various stages include tetraspanin‐2 and calpain for S. mansoni and paramyosin , triose‐phosphate isomerase , tetraspanin and schistosome insulin receptor for S. japonicum . Many of the vaccines for S. japonicum are being developed to help control the transmission contribution of zoonotic host species.…”

Section: Status Of Vaccine Candidates and The Potential Role In Elimimentioning

confidence: 99%

Immunology of human schistosomiasis

Colley

Secor

2014

Parasite Immunology

225

227

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There is a wealth of immunologic studies that have been carried out in experimental and human schistosomiasis that can be classified into three main areas: immunopathogenesis, resistance to reinfection and diagnostics. It is clear that the bulk of, if not all, morbidity due to human schistosomiasis results from immune-response-based inflammation against eggs lodged in the body, either as regulated chronic inflammation or resulting in fibrotic lesions. However, the exact nature of these responses, the antigens to which they are mounted and the mechanisms of the critical regulatory responses are still being sorted out. It is also becoming apparent that protective immunity against schistosomula as they develop into adult worms develops slowly and is hastened by the dying of adult worms, either naturally or when they are killed by praziquantel. However, as with anti-egg responses, the responsible immune mechanisms and inducing antigens are not clearly established, nor are any potential regulatory responses known. Finally, a wide variety of immune markers, both cellular and humoral, can be used to demonstrate exposure to schistosomes, and immunologic measurement of schistosome antigens can be used to detect, and thus diagnose, active infections. All three areas contribute to the public health response to human schistosome infections.

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Section: Status Of Vaccine Candidates and The Potential Role In Elimimentioning

confidence: 99%

Immunology of human schistosomiasis

Colley

Secor

2014

Parasite Immunology

225

227

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“…Its role as a secreted protein is unclear, although in some pathogenic microbes it has a role in the recognition of cell surface and extracellular matrix glycoproteins [21–24]. Experimental trials are encouraging, with vaccination of several different experimental animals against Schistosoma TPI reducing the level and duration of infection [25–30]. This approach has also been adopted in other parasites.…”

Section: Introductionmentioning

confidence: 99%

Triose phosphate isomerase from the blood fluke Schistosoma mansoni: Biochemical characterisation of a potential drug and vaccine target

Zinsser¹,

Farnell²,

Dunne³

et al. 2013

FEBS Letters

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a b s t r a c tThe glycolytic enzyme triose phosphate isomerase from Schistosoma mansoni is a potential target for drugs and vaccines. Molecular modelling of the enzyme predicted that a Ser-Ala-Asp motif which is believed to be a helminth-specific epitope is exposed. The enzyme is dimeric (as judged by gel filtration and cross-linking), resistant to proteolysis and highly stable to thermal denaturation (melting temperature of 82.0°C). The steady-state kinetic parameters are high (K m for dihydroxyacetone phosphate is 0.51 mM; K m for glyceraldehyde 3-phosphate is 1.1 mM; k cat for dihydroxyacetone phosphate is 7800 s À1 and k cat for glyceraldehyde 3-phosphate is 6.9 s À1). Structured summary of protein interactions:SmTPI and SmTPI bind by cross-linking study (View interaction) SmTPI and SmTPI bind by molecular sieving (View interaction)

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“…This gene encodes a protein that has a prohibitin domain and similarity with stomatin like-protein 2 (SLP-2) from Danio rerio; recently the human SLP-2 has been suggested to be involved in TCR signalling, being proposed as a potential immunotherapeutic target (Kirchhof et al 2008). It is important to note, that in the search for vaccines against Schistosoma, many studies using DNA vaccines initiate with low protective values, which are then increased by the use of adjuvants or prime-boost strategies (Dai et al 2009;Siddiqui et al 2005;Wei et al 2008Wei et al , 2009. The protective effect of Dif 4 was recently confirmed with a recombinant protein formulation, which was able to reduce the worm burden in 30-32% after challenge in the murine model (Farias et al 2010).…”

Section: Discussionmentioning

confidence: 98%

Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates

et al. 2010

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Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to cercaria, we explored the Schistosoma mansoni transcriptome by looking at the relative frequency of reads in EST libraries from both stages. The 400 genes potentially up-regulated in schistosomula were analyzed as to their Gene Ontology categorization, and we have focused on those encoding-predicted proteins with no similarity to proteins of other organisms, assuming they could be parasite-specific proteins important for survival in the host. Up-regulation in schistosomulum relative to cercaria was validated with real-time reverse transcription polymerase chain reaction (RT-PCR) for five out of nine selected genes (56%). We tested their protective potential in mice through immunization with DNA vaccines followed by a parasite challenge. Worm burden reductions of 16-17% were observed for one of them, indicating its protective potential. Our results demonstrate the value and caveats of using stage-associated frequency of ESTs as an indication of differential expression coupled to DNA vaccine screening in the identification of novel proteins to be further investigated as potential vaccine candidates.

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DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica

Cited by 30 publications

References 35 publications

Immunology of human schistosomiasis

Immunology of human schistosomiasis

Triose phosphate isomerase from the blood fluke Schistosoma mansoni: Biochemical characterisation of a potential drug and vaccine target

Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates

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