DNA typing of HLA Class II genes in B‐lymphoblastoid cell lines homozygous for HLA

Kimura, Akinori; Dong, Rui-Ping; Harada, Haruhito; Sasazuki, Takehiko

doi:10.1111/j.1399-0039.1992.tb01951.x

Cited by 205 publications

(102 citation statements)

References 13 publications

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“…Primers used for these amplifications were described in the 11th Histocompatibility Workshop. 25 All of the HLA-DPB1 alleles seen in this study were identifiable by RSCA. Only one allele (HLA-DPB1*5401) had not previously been characterised by RSCA, but the mobility seen for this allele was distinct, and the allele was confirmed by sequence-based typing.…”

Section: Hla Typingmentioning

confidence: 91%

The degree of matching at HLA-DPB1 predicts for acute graft-versus-host disease and disease relapse following haematopoietic stem cell transplantation

Shaw

Potter

Mayor

et al. 2003

Bone Marrow Transplant

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Summary:The importance of matching for HLA-DPB1 in unrelated donor haematopoietic stem cell (HSC) transplantation is little understood. Most transplant centres do not, currently, prospectively match for DPB1, but emerging data show that DPB1 matching does play a role in determining outcome. We studied the impact of HLA-DPB1 matching on outcome in 143 recipients of T-cell depletion transplants, who matched with their respective unrelated donors (allelic level) at HLA-A, -B, -C, -DRB1 and -DQB1. Of those matched at DPB1, 47.2% (17/36) developed acute graft-versus-host disease (aGvHD) as compared to 66.3% (55/83) of those who were mismatched. This led to a 19.1% (95% CI 0.1-38.3%) increase in the chance of developing aGvHD in mismatched patients (P ¼ 0.049). Relapse of the original disease occurred in 51 recipients; 23 of 37 (62%) matched at both DPB1 alleles, 28 of 82 (34%) were mismatched at one or two DPB1 alleles. Thus, there was a significantly higher relapse rate (P ¼ 0.0011) in transplant recipients who matched at both DPB1 alleles. In conclusion, a donor/recipient DPB1 match was associated with a significantly lower incidence of aGvHD and a significantly higher incidence of disease relapse. This study provides further evidence for an immunogenic role of HLA-DPB1 in HSC transplants.

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Section: Hla Typingmentioning

confidence: 91%

The degree of matching at HLA-DPB1 predicts for acute graft-versus-host disease and disease relapse following haematopoietic stem cell transplantation

Shaw

Potter

Mayor

et al. 2003

Bone Marrow Transplant

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“…The HLA-DQA1 and -DQB1 genotypes were available for 11 736 healthy control subjects (genomic marker contributors) for 25 of the countries participating in the EU-RODIAB ACE network. The HLA class II genotyping was done at the local laboratories of the various network centres and was mainly carried out by polymerase chain reaction with subsequent hybridisation to sequence-specific oligonucleotide probes (PCR-SSOP) according to the protocols of the 11 th International HLA Workshop, while some centres used polymerase chain reactions with sequence-specific primers [20,21]. a Standardized incidence rate is based on groups matched for (0±4, 5±9 and 10±14 years) of equal size Table 2.…”

Section: Methodsmentioning

confidence: 99%

Correlations between the incidence of childhood-onset Type I diabetes in Europe and HLA genotypes

Rønningen¹,

Keiding

Green

2001

Diabetologia

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Recent research has identified two major features which influence the frequency and distribution Type I (insulin-dependent) diabetes mellitus in childhood. First, in the northern European countries a steady increase, about 3 % annually in incidence (and hence, disease risk), has been observed, during the last decades [1]. Second, on the global scale Europe has the highest risk of Type I diabetes that has been measured, ranging tenfold from Finland (highest) to South-eastern Europe (lowest) but with striking regional variations [1±3].Type I diabetes is caused by the interaction of genetic factors and environmental factors. The rise in Type I diabetes incidence cannot be solely explained by genetic mechanisms but it is still not known whether the geographical variation in diabetes incidence is attributable to differences in the distributions of environmental factors or genes, or a combination of both. Correlating the degree of population risk of Type I di- Diabetologia (2001) AbstractThere are large variations in the incidence of Type I

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“…20 The first typing of 67 uncomplicated plus 39 protracted forms of acute HAV infection was performed with a resolution corresponding to standard serologic typing (intermediate-resolution), and results were compared with a panel of 293 ethnically matched normal controls. High-resolution typing was performed for the subtypes of DRB1*13 and DQB1 by the SSP method (FastypeTM System, Biosynthesis, Lewisville, TX), which showed frequency difference between patients and controls.…”

Section: Methodsmentioning

confidence: 99%

Protracted, but not acute, hepatitis A virus infection is strongly associated with HLA-DRB1*1301, a marker for pediatric autoimmune hepatitis

Velasco²,

et al. 2001

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HLA alleles are known to be associated with susceptibility to develop autoimmune hepatitis (AH), and hepatitis A virus (HAV) infection is postulated as a putative trigger for AH. We investigated whether HLA may influence the outcome of the HAV infection by studying 67 children with self-limited and 39 children with protracted forms of this infection. HLA typing of the uncomplicated forms showed no significant increase of any HLA class I or II alleles. In contrast, DRB1*1301 was present in 46.1% of the children with protracted forms (vs. 9.8% in healthy controls; relative risk [RR]: 7.6; 2 ‫؍‬ 33.3; P ‫؍‬ 2 ؋ 10 ؊9 ). In uncomplicated hepatitis, 45% developed anti-smooth muscle antibody (SMA)/actin antibodies, but only 1 child had detectable antibodies after 3 months of infection onset. In contrast, after 1 year, 69% of the patients suffering protracted forms had titers of anti-SMA/actin antibodies that ranged between 1:40 and 1:160. Within their follow-up, 2 patients developed a Hashimoto's thyroiditis, but the remaining patients showed no signs of developing autoimmune hepatitis. We conclude that the DRB1*1301 haplotype is strongly associated with the protracted forms of HAV infection and suggest that the infection allows a sustained release of liver self-antigens. However, other still-unknown susceptibility genes are required for the full development of pediatric AH. (HEPATOLOGY 2001;33:1512-1517

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DNA typing of HLA Class II genes in B‐lymphoblastoid cell lines homozygous for HLA

Cited by 205 publications

References 13 publications

The degree of matching at HLA-DPB1 predicts for acute graft-versus-host disease and disease relapse following haematopoietic stem cell transplantation

The degree of matching at HLA-DPB1 predicts for acute graft-versus-host disease and disease relapse following haematopoietic stem cell transplantation

Correlations between the incidence of childhood-onset Type I diabetes in Europe and HLA genotypes

Protracted, but not acute, hepatitis A virus infection is strongly associated with HLA-DRB1*1301, a marker for pediatric autoimmune hepatitis

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