DNA typing and genetic relations among populations of Kelussia odoratissima using ISSR and SRAP markers

Hadian, Javad; Raeisi, Safiollah; Nadjafi, Farsad; Khadivi, Ali

doi:10.1007/s00606-013-0979-3

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…However, an internal transcribed spacer (ITS) region analysis of Angelica cultivars showed that they are different from each other genetically and they are easily distinguishable [31]. ISSR analysis is another important molecular technique for the genetic characterization [13,15,26,32]. To verify the improved RAPD analysis result of our study, we employed the ISSR technique for amplifying DNA materials of all ten Angelica samples and analyzed the results by cluster dendrogram.…”

Section: Discussionmentioning

confidence: 88%

“…In the era of modern biotechnology, several molecular techniques have been developed for the genetic studies and characterizations of different organisms, among which random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) analysis are well established and widely used [12,13,14,15]. RAPD is a more reliable molecular technique for the genetic characterization of organisms, especially of plants.…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

Mei

Zhang

Zhu

et al. 2015

Electronic Journal of Biotechnology

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Background: Angelica sinensis is a well-known traditional Chinese medicinal plant. We aimed to assess the genetic diversity and relationships in A. sinensis cultivars collected from different locations of China and also some other Angelica species. Results: We employed an improved random amplified polymorphic DNA (RAPD) technique for the amplification of DNA materials from ten Angelica cultivars, and the results were verified by inter-simple sequence repeat (ISSR) analysis. Twenty six RAPD primers were used for RAPD, and the amplified bands were found highly polymorphic (96%). Each primer amplified 8-14 bands with an average of 10.25. The cluster dendrogram showed that the similarity coefficients ranged from 0.41 to 0.92. The similarity coefficients were higher among different cultivars of A. sinensis, and lower among different species. Twenty ISSR primers were used for the amplification, and each primer generated 6-10 bands with an average of 7.2 bands per primer. The cluster dendrogram showed that the similarity coefficients ranged from 0.35 to 0.89. Conclusions: This study genetically characterized the Angelica species, which might have a significant contribution to the genetic and ecological conservation of this important medicinal plant. Also, this study indicates that the improved RAPD and ISSR analyses are important and potent molecular tools for the study of genetic diversity and authentication of organisms.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

Mei

Zhang

Zhu

et al. 2015

Electronic Journal of Biotechnology

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“…PIC = 0.32, Rp = 7.80, and MI = 3.48 generated by ISSR primers were higher than that of sequence‐related amplified polymorphism (SRAP) analysis ( PIC = 0.30, Rp = 5.61, and MI = 1.88). The effectiveness of ISSR compared to the SRAP markers for assessing the degree of genetic variation in Kelussia odoratissima has already been confirmed . Also, PIC value using ISSR markers ranged from 0.22 to 0.36 and MI ranged from 1.10 to 2.88 per primer in Satureja rechingeri…”

Section: Resultsmentioning

confidence: 99%

“…The effectiveness of ISSR compared to the SRAP markers for assessing the degree of genetic variation in Kelussia odoratissima has already been confirmed. [65] Also, PIC value using ISSR markers ranged from 0.22 to 0.36 and MI ranged from 1.10 to 2.88 per primer in Satureja rechingeri. [66] Essential Oils Content…”

Section: Unweighted Pair-group Methods With Arithmetic Mean (Upgma) CLmentioning

confidence: 99%

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Pourhosseini

Hadian

Sonboli³

et al. 2018

Chemistry & Biodiversity

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Genetic and the essential oil composition variability among twelve Perovskia abrotanoides populations (PAbPs) growing wild in Iran were assessed by ISSR markers, GC-FID and GC/MS, respectively. Nine selected ISSR primers produced 119 discernible bands, of them 96 (80.7%) being polymorphic. Genetic similarity values among populations ranged between 0.07 and 0.79 which indicated a high level of genetic variation. Polymorphic information content, resolving power and marker index generated by ISSR primers were, 0.31, 6.14, and 3.32, respectively. UPGMA grouped PAbPs into four main clusters. Altogether, 38 chemical compounds were identified in the oils, and a relatively high variation in their contents was found. Camphor (11.9 - 27.5%), 1,8-cineole (11.3 - 21.3%), α-bisabolol (0.0 - 13.1%), α-pinene (5.9 - 10.8%), and δ-3-carene (0.1 - 10.5%) were the major compounds. Oxygenated monoterpenes (32.1 - 35.8%) and monoterpene hydrocarbons (25.7 - 30.4%) were the main groups of compounds in the oils studied. Cluster analysis and principal-component analysis were used to characterize the samples according to oil components. Four main chemotypes were found to be Chemotype I (camphor/1,8-cineol), Chemotype II (1,8-cineole/camphor), Chemotype III (camphor/1,8-cineol/α-bisabolol), and Chemotype IV (camphor/δ-3-carene/α-bisabolol). The information, provided here on P. abrotanoides populations, will be useful to introduce this plant into agricultural systems.

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“…Because ISSR profiling detects random inter-simple sequence repeats in the genome and SRAP profiling reflects sequence variation within gene coding regions, there is no overlap between the principles of the two methods. Attempts to combine the two methods to identify taxonomic entities have produced inconclusive results [ 60 , 62 ]. Similarly, our attempt to combine the two methods did not improve the resolution.…”

Section: Discussionmentioning

confidence: 99%

Virulence Structure of Blumeria graminis f. sp. tritici and Its Genetic Diversity by ISSR and SRAP Profiling Analyses

Liu

Gong

et al. 2015

PLoS ONE

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Blumeria graminis f. sp. tritici, which causes wheat powdery mildew, is an obligate biotrophic pathogen that can easily genetically adapt to its host plant. Understanding the virulence structure of and genetic variations in this pathogen is essential for disease control and for breeding resistance to wheat powdery mildew. This study investigated 17 pathogenic populations in Sichuan, China and classified 109 isolates into two distinct groups based on pathogenicity analysis: high virulence (HV, 92 isolates) and low virulence (LV, 17 isolates). Populations from Yibin (Southern region), Xichang (Western region), and Meishan (Middle region) showed lower virulence frequencies than populations from other regions. Many of the previously known resistance genes did not confer resistance in this study. The resistance gene Pm21 displayed an immune response to pathogenic challenge with all populations in Sichuan, and Pm13, Pm5b, Pm2+6, and PmXBD maintained resistance. AMOVA revealed significantly higher levels of variation within populations and lower levels of variation among populations within regions. High levels of gene flow were detected among populations in the four regions. Closely related populations within each region were distinguished by cluster analyses using ISSR and SRAP alleles. Both ISSR and SRAP allele profiling analyses revealed high levels of genetic diversity among pathogenic populations in Sichuan. Although ISSR and SRAP profiling analysis showed similar resolutions, the SRAP alleles appeared to be more informative. We did not detect any significant association between these alleles and the virulence or pathogenicity of the pathogen. Our results suggest that ISSR and SRAP alleles are more efficient for the characterization of small or closely related populations versus distantly related populations.

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DNA typing and genetic relations among populations of Kelussia odoratissima using ISSR and SRAP markers

Cited by 6 publications

References 27 publications

Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Virulence Structure of Blumeria graminis f. sp. tritici and Its Genetic Diversity by ISSR and SRAP Profiling Analyses

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