DNA-Templated Protein Arrays for Single-Molecule Imaging

Selmi, Daniele N.; Adamson, R.J.; Attrill, Helen; Goddard, Alan D.; Gilbert, Robert J.C.; Watts, Anthony; Turberfield, Andrew J.

doi:10.1021/nl1037769

Cited by 94 publications

(96 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[10][11][12] The computational tools [11][12][13] for designing such objects have emerged along with these techniques, and this progress has opened up new possibilities for the researchers to effortlessly build their own nanostructures for tailored uses. [14] Recently demonstrated applications based on customized DNA nanostructures include artificial ion channels, [15] optical (plasmonic and photonic) structures, [16,17] high-precision molecular positioning devices, [18] modifiable templates for arranging, e.g., proteins, [19][20][21] polymers, [22] and nanotubes, [23] as well as DNA-assisted techniques for creating arbitrarily shaped metal nanoparticles. [24][25][26] Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms.…”

mentioning

confidence: 99%

Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility

Auvinen

Zhang

Nonappa

et al. 2017

Adv Healthcare Materials

174

144

View full text Add to dashboard Cite

DNA nanotechnology has taken a giant leap toward real-life applications during the recent years. [1,2] After the invention of DNA origami in 2006, [3] the whole research field has grown exponentially. [2,4] Today there are numerous ways to build discrete user-defined, accurate, and fully addressable DNA nanostructures, such as scaffolded 2D and 3D origami [3,5,6] with twists, curves, and bends, [7,8] Lego-like objects formed from molecular canvases, [9] and wireframe-based meshed constructions. [10][11][12] The computational tools [11][12][13] for designing such objects have emerged along with these techniques, and this progress has opened up new possibilities for the researchers to effortlessly build their own nanostructures for tailored uses. [14] Recently demonstrated applications based on customized DNA nanostructures include artificial ion channels, [15] optical (plasmonic and photonic) structures, [16,17] high-precision molecular positioning devices, [18] modifiable templates for arranging, e.g., proteins, [19][20][21] polymers, [22] and nanotubes, [23] as well as DNA-assisted techniques for creating arbitrarily shaped metal nanoparticles. [24][25][26] Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. This study shows that DNA origami can be coated by precisely defined oneto-one protein-dendron conjugates to tackle the aforementioned issues. The dendron part of the conjugate serves as a cationic binding domain that attaches to the negatively charged DNA origami surface via electrostatic interactions. The protein is attached to dendron through cysteinemaleimide bond, making the modular approach highly versatile. This work demonstrates the coating using two different proteins: bovine serum albumin (BSA) and class II hydrophobin (HFBI). The results reveal that BSA-coating significantly improves the origami stability against endonucleases (DNase I) and enhances the transfection into human embryonic kidney (HEK293) cells. Importantly, it is observed that BSA-coating attenuates the activation of immune response in mouse primary splenocytes. Serum albumin is the most abundant protein in the blood with a long circulation half-life and has already found clinically approved applications in drug delivery. It is therefore envisioned that the proposed system can open up further opportunities to tune the properties of DNA nanostructures in biological environment, and enable their use in various delivery applications.

show abstract

mentioning

confidence: 99%

Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility

Auvinen

Zhang

Nonappa

et al. 2017

Adv Healthcare Materials

174

144

View full text Add to dashboard Cite

show abstract

“…81 Turberfield and co-workers used periodic 2D DNA tile arrays as templates to arrange proteins and subsequently used cryo-EM to solve their structures. 82 …”

Section: Recent Developments In Structural Dna Nanotechnologymentioning

confidence: 99%

Structural DNA Nanotechnology: State of the Art and Future Perspective

et al. 2014

View full text Add to dashboard Cite

Over the past three decades DNA has emerged as an exceptional molecular building block for nanoconstruction due to its predictable conformation and programmable intra- and intermolecular Watson–Crick base-pairing interactions. A variety of convenient design rules and reliable assembly methods have been developed to engineer DNA nanostructures of increasing complexity. The ability to create designer DNA architectures with accurate spatial control has allowed researchers to explore novel applications in many directions, such as directed material assembly, structural biology, biocatalysis, DNA computing, nanorobotics, disease diagnosis, and drug delivery. This Perspective discusses the state of the art in the field of structural DNA nanotechnology and presents some of the challenges and opportunities that exist in DNA-based molecular design and programming.

show abstract

“…The potential of DNA nanotechnology, however, is obvious. Seeman's original motivation was to use DNA crystals to conjugate proteins and facilitate crystallography; origami bundles and two-dimensional lattices have indeed been used to aid protein structure determination 55,56 . Similarly, finite-sized nanostructures contain unique strands at well-defined locations.…”

Section: Applications Of Dna Nanotechnologymentioning

confidence: 99%

DNA nanotechnology: understanding and optimisation through simulation

Ouldridge

2014

Molecular Physics

View full text Add to dashboard Cite

DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended 'overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

show abstract

DNA-Templated Protein Arrays for Single-Molecule Imaging

Cited by 94 publications

References 22 publications

Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility

Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility

Structural DNA Nanotechnology: State of the Art and Future Perspective

DNA nanotechnology: understanding and optimisation through simulation

Contact Info

Product

Resources

About