DNA synthesized in Escherichia coli K12 (λ) after infection with an rII mutant of bacteriophage T4

Nomura, Masayasu

doi:10.1016/0042-6822(61)90191-x

Cited by 12 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deoxycytidine triphosphatase, an early enzyme involved in phage DNA synthesis, is produced at the same rate up to about 12 min after infection of K(X) with T4r+ or T4rII phage. Phage DNA is synthesized up to 10 to 15 min in rll-infected K(X) (11,13,20). In contrast, no mature phage particles are produced, nor is any anti-phage serum-blocking antigen or phage lysozyme.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophate-Induced Functions in Escherichia coli K(λ) Infected with rII Mutants of Bacteriophage T4

Rutberg

1966

J Bacteriol

View full text Add to dashboard Cite

RUTBERG, BLANKA (Karolinska Institutet, Stockholm, Sweden), AND LARS RUTBERG. Bacteriophage-induced functions in Escherichia coli K(X) infected with rII mutants of bacteriophage T4. J. Bacteriol. 91:76-80. 1966.-When Escherichia coli K(X) was infected with rII mutants of phage T4, deoxycytidine triphosphatase, one of the phage-induced early enzymes, was produced at initially the same rate as in r+-infected cells. Deoxyribonuclease activity was one-third to one-half of that of r+infected cells. This lower deoxyribonuclease activity was observed also in other hosts or when infection was made with rI or rIII mutants. Presence of chloramphenicol did not allow a continued synthesis of phage deoxyribonucleic acid in rIh-infected K(X). No phage lysozyme was detected nor was any antiphage serum-blocking antigen found in rIh-infected K(X). It is suggested that the rII gene is of significance for the expression of phage-induced late functions in the host K(X). The structure of the rII gene of phage T4 is known in its most intimate details (4), but the physiology of the rII gene is still largely unknown (9, 11, 13, 20). The aim of the present work was to study some phage-specific products induced by infection with T4rII of its nonpermissive host Escherichia coli K(X). Production of deoxycytidine triphosphatase and deoxyribonuclease was chosen to represent early functions of the phage genome; among late functions, phage lysozyme and antiphage serum-blocking antigen were studied. In the experiments concerning deoxyribonuclease activity, rI and rIII mutants were included. MATERIALS AND METHODS Bacterial strains. E. coli B, E. coli Bc (E. coli B cured from its defective prophage) E. coli K (C 600 of Appleyard (3)), and E. coli K(X). The bacterial strains were kindly provided by G. Bertani.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacteriophate-Induced Functions in Escherichia coli K(λ) Infected with rII Mutants of Bacteriophage T4

Rutberg

1966

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Deoxyribonuclease activity was about one-third to one-half of that of r+-infected cells, but no lysozyme activity was detected in rII-infected K(X) cells. Other reports show that the initiation of deoxyribonucleic acid (DNA) synthesis is normal in rll-infected K-12(X), but DNA synthesis is blocked at a later stage (4,5,8). In a permissive host, phage lysozyme synthesis starts at about the same time as the initiation of DNA synthesis (7).…”

mentioning

confidence: 99%

Bacteriophage Lysozyme Synthesis in Escherichia coli K-12(λ) Infected with rII Mutant of Bacteriophage T4

Mark

1972

J Virol

View full text Add to dashboard Cite

show abstract

Viren und Phagen

Wittmann¹,

Harm²

1962

Progress in Botany

View full text Add to dashboard Cite

DNA synthesized in Escherichia coli K12 (λ) after infection with an rII mutant of bacteriophage T4

Cited by 12 publications

References 4 publications

Bacteriophate-Induced Functions in Escherichia coli K(λ) Infected with rII Mutants of Bacteriophage T4

Bacteriophate-Induced Functions in Escherichia coli K(λ) Infected with rII Mutants of Bacteriophage T4

Bacteriophage Lysozyme Synthesis in Escherichia coli K-12(λ) Infected with rII Mutant of Bacteriophage T4

Viren und Phagen

Contact Info

Product

Resources

About