DNA synthesis in nuclei and nuclear matrices of regenerating rat liver: Effect of whole-body gamma irradiation

Davé, Vibhuti P.; Patil, M. S.; Pandey, Virendra N.; Pradhan, D.S.

doi:10.1007/bf01210511

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…The enzymes were assayed according to the procedure standardized in our lab [26]. DNA polymerase activity was measured in purified nuclear preparations using ␣[ 32 P]dATP with endogenous DNA as the template primer [27]. Aphidicolin was used to inhibit replicative DNA synthesis so that aphidicolin-resistant activity represented b-polymerase.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Damage to DNA and activity of nuclear DNA repair and replicative enzymes following N‐nitrosodiethylamine treatment to rats

Pasupathy

Bhattacharya

2000

J. Biochem. Mol. Toxicol.

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Continuous administration in the drinking water of hepatocarcinogen N-nitrosodiethylamine (NDEA) to male rats (200 mg/L) for 60 days resulted in DNA damage in the form of single strand breaks. The damage, which is measured as a shift in the sedimentation of DNA in alkaline sucrose density gradients, was found to be maximum at the fourth week of treatment, and the sedimentation pattern of DNA was found to return to near normal size by the seventh week of NDEA treatment. Simultaneously, there were perturbations in the nuclear enzymes involved in DNA replication and repair. Activities of DNA polymerase b, DNA ligase, and topoisomerase were found to increase in as early as the first week of NDEA treatment and reached the maximum at the fourth week, and thereafter declined to normal level by the eighth week of treatment. Concomitantly, the activities of DNA polymerase ␣, DNA primase, and RNA polymerase which were unaltered in the initial period of carcinogen treatment recorded a marked increase after sixth week of NDEA treatment. Results suggest that administration of NDEA inflicts DNA damage, which is manifested as increase in DNA repair enzymes in the initial period and activated DNA replicative enzymes at a later period, indicating the active proliferation of transformed cells.

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Section: Enzyme Assaysmentioning

confidence: 99%

Damage to DNA and activity of nuclear DNA repair and replicative enzymes following N‐nitrosodiethylamine treatment to rats

Pasupathy

Bhattacharya

2000

J. Biochem. Mol. Toxicol.

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show abstract

“…DNA polymerase activity was measured in purified nuclear preparations using ␣[ 32 P]dATP with endogenous DNA as the template primer [27]. Aphidicolin was used to inhibit replicative DNA synthesis so that aphidicolin-resistant activity represented b-polymerase.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Damage to DNA and activity of nuclear DNA repair and replicative enzymes followingN-nitrosodiethylamine treatment to rats

Pasupathy

Bhattacharya

2000

J. Biochem. Mol. Toxicol.

View full text Add to dashboard Cite

Continuous administration in the drinking water of hepatocarcinogen N‐nitrosodiethylamine (NDEA) to male rats (200 mg/L) for 60 days resulted in DNA damage in the form of single strand breaks. The damage, which is measured as a shift in the sedimentation of DNA in alkaline sucrose density gradients, was found to be maximum at the fourth week of treatment, and the sedimentation pattern of DNA was found to return to near normal size by the seventh week of NDEA treatment. Simultaneously, there were perturbations in the nuclear enzymes involved in DNA replication and repair. Activities of DNA polymerase β, DNA ligase, and topoisomerase were found to increase in as early as the first week of NDEA treatment and reached the maximum at the fourth week, and thereafter declined to normal level by the eighth week of treatment. Concomitantly, the activities of DNA polymerase α, DNA primase, and RNA polymerase which were unaltered in the initial period of carcinogen treatment recorded a marked increase after sixth week of NDEA treatment. Results suggest that administration of NDEA inflicts DNA damage, which is manifested as increase in DNA repair enzymes in the initial period and activated DNA replicative enzymes at a later period, indicating the active proliferation of transformed cells. © 2000 John Wiley & Sons, Inc. J Biochem Toxicol 14:277–282, 2000

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“…However, the technique requires sine qua non that normal hepatocytes exposed to BNCT at dose levels that would be typically encountered in normal tissue during therapy should be able to regenerate healthy liver following a regenerative stimulus. It is known that radiation inhibits the regenerative process induced by subtotal hepatectomy as described by Dave et al (1991) and Geraci and Mariano (1994) for 10 and 15 Gy of photon irradiation respectively. These authors studied the effect on liver regeneration of radiation in previously subtotally hepatectomized animals rather than the effect of radiation followed by partial hepatectomy 21 days later, as in the present study.…”

Section: Introductionmentioning

confidence: 99%

Effect of Boron Neutron Capture Therapy (BNCT) on normal liver regeneration: Towards a novel therapy for liver metastases

Cardoso

Trivillin

Heber

et al. 2007

International Journal of Radiation Biology

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DNA synthesis in nuclei and nuclear matrices of regenerating rat liver: Effect of whole-body gamma irradiation

Cited by 6 publications

References 28 publications

Damage to DNA and activity of nuclear DNA repair and replicative enzymes following N‐nitrosodiethylamine treatment to rats

Damage to DNA and activity of nuclear DNA repair and replicative enzymes following N‐nitrosodiethylamine treatment to rats

Damage to DNA and activity of nuclear DNA repair and replicative enzymes followingN-nitrosodiethylamine treatment to rats

Effect of Boron Neutron Capture Therapy (BNCT) on normal liver regeneration: Towards a novel therapy for liver metastases

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