DNA Strand-Transfer Activity in Pea (Pisum sativum L.) Chloroplasts

Cerutti, Heriberto; Jagendorf, André T.

doi:10.1104/pp.102.1.145

Cited by 33 publications

(17 citation statements)

References 37 publications

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“…A number of recent reports indicate that a recA homolog functions in this way in plant chloroplasts (33)(34)(35)(36). To test this hypothesis, we preincubated mammalian mitochondrial extracts with control antisera, or immunoglobulin that had been affinity purified from recA antisera, using an Arabidopsis thaliana chloroplast recA homolog column (affinity purified antisera was kindly provided to us by Dr. A. Jagendorf, Cornell University).…”

Section: Mitochondrial Extract Hr Activity Is Not the Results Of Nuclementioning

confidence: 99%

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Mammalian Mitochondria Possess Homologous DNA Recombination Activity

Thyagarajan

Padua²,

Campbell

1996

Journal of Biological Chemistry

253

132

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Mitochondrial protein extracts from normal and immortalized mammalian somatic cells catalyze homologous recombination of plasmid DNA substrates. Mitochondrial homologous recombination activity required exogenous adenosine triphosphate, although substantial activity remained when non-hydrolyzable analogs were used instead. There was no requirement for added nucleoside triphosphates, and the reaction was not inhibited by dideoxyadenosine triphosphate or aphidicolin. The majority of recombinant plasmid molecules result from a conservative process, indicating that nuclease-mediated strand-annealing is not responsible for the mitochondrial homologous recombination activity. Affinity-purified anti-recA antibodies inhibited the reaction, suggesting that activity is dependent on a mammalian mitochondrial homolog of the bacterial strand-transferase protein. The presence of homologous recombination activity within mammalian mitochondrial extracts suggests that this process is involved in mitochondrial DNA repair.

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Section: Mitochondrial Extract Hr Activity Is Not the Results Of Nuclementioning

confidence: 99%

“…Second, a number of nuclear-encoded yeast genes have been shown to be involved in mitochondrial recombination and repair (31,32). Finally, studies of DNA repair in plant chloroplasts have shown that a homolog of the bacterial recA gene is involved in DNA repair in that organelle (33)(34)(35)(36).…”

mentioning

confidence: 99%

Mammalian Mitochondria Possess Homologous DNA Recombination Activity

Thyagarajan

Padua²,

Campbell

1996

Journal of Biological Chemistry

253

132

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“…In addition, a plant Arabidopsis thaliana gene encoding a RecA homologue, which is distinct from Rad51/Dmc1 and closely related to bacterial RecA proteins, has been characterized and found to be targeted to chloroplasts (At1g79050 [AtcprecA]; Cerutti et al, 1992, Cao et al, 1997. RecA-like strand transfer activity was also detected in a stromal extract from pea chloroplast (Cerutti and Jagendorf, 1993). The complete nuclear genome sequence of A. thaliana (The Arabidopsis Genome Initiative, 2000) revealed the presence of two other recA genes (At3g10140 [AtmtrecA] and At2g19490) related to bacterial recA, and At3g10140 was reported to be targeted to mitochondria (Khazi et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Involvement of mitochondrial-targeted RecA in the repair of mitochondrial DNA in the moss, Physcomitrella patens

et al. 2007

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Homologous recombination is a universal process that contributes to genetic diversity and genomic integrity. Bacterial-type RecA generally exists in all bacteria and plays a crucial role in homologous recombination. Although RecA homologues also exist in plant mitochondria, there have been few reports about the in vivo functions of these homologues. We identified a recA gene orthologue (named PprecA1) in a cDNA library of the moss, Physcomitrella patens. N-terminal fusion of the putative organellar targeting sequence of PpRecA1 to GFP caused a targeting of PpRecA1 to mitochondria. PprecA1 partially complemented the effects of a DNA damaging agent in an Escherichia coli recA deficient strain. Additionally, the expression of PprecA1 was induced by treating the plants with DNA damaging agents. Disruption of PprecA1 by targeted replacement resulted lower rate of the recovery of the mitochondrial DNA from methyl methan sulfonate damage. This is the first report about the characteristics of a null mutant of bacterial-type recA gene in plant. The data suggest that PprecA1 participates in the repair of mitochondrial DNA in P. patens.

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“…The higher plant Arabidopsis thaliana features Rad51 and Dmc1, eukaryotic counterparts for nuclear DNA recombination/repair (Bishop et al, 1992;Shinohara et al, 1992), and several bacterial-type RecA homologs encoded in the nucleus, which have been shown to be targeted to chloroplasts (Cerutti et al, 1992;Cao et al, 1997) and/or mitochondria (Khazi et al, 2003;Shedge et al, 2007). RecA-like strand transfer activity has been detected in a stromal extract from pea (Pisum sativum) chloroplasts (Cerutti and Jagendorf, 1993) and in soybean (Glycine max) mitochondria (Manchekar et al, 2006). In the moss Physcomitrella patens, two RecA homologs were identified, a mitochondrial-targeted RecA (RECA1) and a chloroplasttargeted RecA (RECA2) Inouye et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

Odahara

Kuroiwa

et al. 2009

The Plant Cell

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RecA and its ubiquitous homologs are crucial components in homologous recombination. Besides their eukaryotic nuclear counterparts, plants characteristically possess several bacterial-type RecA proteins localized to chloroplasts and/or mitochondria, but their roles are poorly understood. Here, we analyzed the role of the only mitochondrial RecA in the moss Physcomitrella patens. Disruption of the P. patens mitochondrial recA gene RECA1 caused serious defects in plant growth and development and abnormal mitochondrial morphology. Analyses of mitochondrial DNA in disruptants revealed that frequent DNA rearrangements occurred at multiple loci. Structural analysis suggests that the rearrangements, which in some cases were associated with partial deletions and amplifications of mitochondrial DNA, were due to aberrant recombination between short (<100 bp) direct and inverted repeats in which the sequences were not always identical. Such repeats are abundant in the mitochondrial genome, and interestingly many are located in group II introns. These results suggest that RECA1 does not promote but rather suppresses recombination among short repeats scattered throughout the mitochondrial genome, thereby maintaining mitochondrial genome stability. We propose that RecA-mediated homologous recombination plays a crucial role in suppression of short repeat-mediated genome rearrangements in plant mitochondria.

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DNA Strand-Transfer Activity in Pea (Pisum sativum L.) Chloroplasts

Cited by 33 publications

References 37 publications

Mammalian Mitochondria Possess Homologous DNA Recombination Activity

Mammalian Mitochondria Possess Homologous DNA Recombination Activity

Involvement of mitochondrial-targeted RecA in the repair of mitochondrial DNA in the moss, Physcomitrella patens

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

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