DNA stiffening and elongation caused by the binding of ethidium bromide

Reinert, K.E.

doi:10.1016/0005-2787(73)90004-x

Cited by 68 publications

(18 citation statements)

References 18 publications

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“…The investigations were carried out under simulated physiological pH conditions (8.0) at 25 ± 0.1°C. The effects of complexes 1 – 6 on the viscosity of salmon testes DNA were investigated alongside a known intercalating agent, ethidium bromide (EB) . The relative viscosity of DNA with increasing concentrations of the silver(I) complexes and EB is shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

Synthesis, characterization, antimicrobial screening and DNA binding of novel silver(I)–thienylterpyridine and silver(I)–furylterpyridine complexes

Njogu

Martincigh

Omondi

et al. 2018

Applied Organom Chemis

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Six new silver(I) complexes, [(AgL1) 2 ](ClO 4 ) 2 (1), [(AgOTfL1) 2 ] (2), [(AgO 2 CF 3 L1) 2 ] (3), [AgL2](ClO 4 ) 2 (4), [(AgOTfL2) 2 ] (5) and [(AgO 2 CF 3 L2) 2 ] (6), were synthesized by reaction of 4′-(2-thienyl)-2,2′;6′,2″-terpyridine (L1)or 4′-(2-furyl)-2,2′;6′,2″-terpyridine (L2) with the respective silver salts, i.e. AgClO 4 , AgOTf or AgTFA. The complexes are dinuclear of the type Ag 2 L 2 in which the two ligands utilize the pyridinyl N atoms from the central rings and one of the peripheral rings to coordinate to one of the silver(I) centres while the remaining pyridinyl N atoms coordinate to the second silver(I) centre. Complexes 2 and 5 have the trifluoromethanesulfonate anion remotely coordinating via a single O atom while complex 3 has the trifluoroacetate anion coordinating to a single silver(I) centre in a bidentate mode. The perchlorate anion in complex 4 is excluded from the silver(I) coordination sphere.All complexes demonstrate moderate to high antimicrobial activity against the Gram-negative bacteria Escherichia coli and Salmonella typhimirium and the fungus Candida albicans. Complexes 1-6 interact strongly with salmon testes DNA as indicated by the steady increase in the relative viscosity of DNA solutions with increasing concentration of the silver(I) complexes and the relatively large intrinsic binding constants measured. There was substantial hyperchromism and hypsochromism recorded in solutions of complexes 1-6 with increasing concentration of salmon testes DNA. Complexes 1-6 interact with DNA via intercalation, and groove and external binding.

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Section: Resultsmentioning

confidence: 99%

Synthesis, characterization, antimicrobial screening and DNA binding of novel silver(I)–thienylterpyridine and silver(I)–furylterpyridine complexes

Njogu

Martincigh

Omondi

et al. 2018

Applied Organom Chemis

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“…With this drug the changes in contour length for both polynucleotides were much smaller, yielding least-squares lines having slopes well below the predicted value of 2 or the experimental value of 1.79 reported for calf thymus DNA [17]. Fig.4.…”

Section: Resultsmentioning

confidence: 67%

“…The viscometric technique is well established as a method for investigating the extension of the DNA helix associated with intercalation [15][16][17][18]. Under conditions where the DNA molecules behave as stiff rods:…”

Section: Methodsmentioning

confidence: 99%

Changes in contour length of polydeoxynucleotide fragments

Fox¹,

Harrison²,

Waring³

1981

FEBS Letters

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“…The straight line plotted represents a least-squares fit to all the data points; its slope is 3.74, which is typical of the values reported for bifunctional intercalators (Waring, 1979). It falls slightly below the theoretical line for idealized bifunctional intercalation, which predicts the relationship L/Lo = 1 + 4r, but its gradient is well over twice the value seen for ethidium (Reinert, 1973). However, the line does not pass through 1.00 and there is an apparent initial decrease in L/Lo.…”

Section: Viscometrymentioning

confidence: 84%