DNA stabilized silver nanoclusters for ratiometric and visual detection of Hg2+ and its immobilization in hydrogels

MacLean, James L.; Morishita, Kiyoshi; Liu, Juewen

doi:10.1016/j.bios.2013.04.002

Cited by 101 publications

(41 citation statements)

References 26 publications

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“…have been reported previously to template AgNCs with different emission properties [1,46]. For each sequence, the emission spectrum was measured at the excitation maximum in the visible region ( Figure 4).…”

Section: Changing the Template Dna Sequence It Is Well-established Tmentioning

confidence: 99%

Towards understanding of poly-guanine activated fluorescent silver nanoclusters

et al. 2014

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It has been recently reported that the fluorescence of some DNA templated silver nanoclusters (AgNCs) can be significantly enhanced upon hybridizing with a partially complementary DNA containing a Grich overhang near the AgNCs. This discovery has found a number of analytical applications but many fundamental questions remain to be answered. In this work, the photostability of these activated AgNCs is reported. After adding the G-rich DNA activator, the fluorescence intensity peaks in ~1 h and then starts to decay, where the decaying rate is much faster with light exposure. The lost fluorescence is recovered by adding NaBH4, suggesting that the bleaching is an oxidative process.Once activated, the G-rich activator can be removed while the AgNCs still maintain most of their fluorescence intensity. UV-vis spectroscopy suggests that new AgNC species are generated upon hybridization with the activator. The base sequence and length of the template DNA have also been varied, leading to different emission colors and color change after hybridization. G-rich aptamers can also serve as activators. Our results indicate that activation of the fluorescence by G-rich DNA could be a convenient method for biosensor development since the unstable NaBH4 is not required for the activation step.

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Section: Changing the Template Dna Sequence It Is Well-established Tmentioning

confidence: 99%

Towards understanding of poly-guanine activated fluorescent silver nanoclusters

et al. 2014

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“…14). Recently, MacLean et al [102] reported a ratiometric detection of Hg 2+ using DNA stabilized Ag NCs with two emission peaks. The 620 nm red peak was quenched by Hg 2+ while the 520 nm green peak increased.…”

Section: Science China Materialsmentioning

confidence: 99%

Recent developments on nanomaterials-based optical sensors for Hg2+ detection

Duan

Zhan

2015

Sci. China Mater.

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Mercuric ion (Hg 2+ ), released from both natural and industrial sources, has severe adverse effects on human health and the environment even at very low concentrations. It is very important to develop a rapid and economical method for the detection of Hg 2+ with high sensitivity and selectivity. Nanomaterials with unique size and shape-dependent optical properties are attractive sensing materials. The application of nanomaterials to design optical sensors for Hg 2+ provides a powerful method for the trace detection of Hg 2+ in the environment, because these optical sensors are simple, rapidly responsive, cost-effective and highly sensitive. This review summarizes the recent advances on the development of optical assays for Hg 2+ in aqueous solution by using functionalized nanomaterials (including noble metal nanoparticles, fluorescent metal nanoclusters, semiconductor quantum dots and carbon nanodots). Detection strategies based on the Hg 2+ -induced changes in spectral absorbance, fluorescence intensity and surface-enhanced Raman scattering signals were described. And the design principles for each optical assay were presented. In addition, the future challenge and the prospect of the development of nanomaterial optical sensors for Hg 2+ detection were also discussed.

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“…In recent years, highly luminescent and stable nanoclusters have been extensively reported as fluorescence probes for a wide range of targets, including small ions [12,13], small molecules such as biothiols [14], hydrogen peroxide [15,16], glucose [17], amino acids [18], as well as nucleic acids [19], proteins [20] and other biological macromolecules [21,22]. Most of the detections were based on either the enhancement or the quenching of the fluorescence.…”

Section: Introductionmentioning

confidence: 99%