DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721

Diver, W.P.; Grinsted, J.; Fritzinger, David C.; Brown, Nigel L.; Altenbuchner, Josef; Rogowsky, Peter; Schmitt, Rüdiger

doi:10.1007/bf00334812

Cited by 92 publications

(45 citation statements)

References 25 publications

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“…Transposons are classified in a particular category on the basis of transposase, resolvase, and terminal IR homologies. Additional criteria include complementation of transposition functions and the direction of transcription of tnpA and tnpR genes (9,10).…”

Section: Discussionmentioning

confidence: 99%

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Evolutionary perspectives on multiresistance beta-lactamase transposons

et al. 1989

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A series of intragenic DNA probes, encoding the major part of the transposase resolvase and inverted repeats of transposons Tn3, Tn2l, and Tn25O1, were used in hybridization assays for homologous DNA sequences in 18 transposons studied. The tnpA and tnpR probes detected extensive homology with Tn3-like and Tn21-like elements for 11 transposons. This high degree of homology was confirmed with the 38-and 48-base-pair inverted-repeat oligonucleotide probes of Tn3, Tn2l, and Tn2S01. The Southern-type gel hybridization experiments localized the tnpA-homologous sequences on the physical DNA maps constructed. The genetic and physical maps of the transposons were compared, as were their nucleic acid sequence homologies. These comparisons suggested a subfamily of mobile elements distinct from but related to the Tn2l group. Based on these results, an evolutionary model is proposed and a pedigree is presented for the genesis of multiresistance ,-lactamase transposons.More than 30 biochemically distinct P-lactamases are known to be plasmid encoded in gram-negative bacteria (21,24,25,28).

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Section: Discussionmentioning

confidence: 99%

“…Their relationship has been examined by complementation of transposition functions and by nucleotide sequencing of transposase (tnpA), resolvase (tnpR), modulator protein (tnpM), and inverted repeats (IR) (9,10,15). For the class 2 members, two main groups are represented by Tn3 and Tn2J, which diverged long ago (9,10,31).…”

mentioning

confidence: 99%

Evolutionary perspectives on multiresistance beta-lactamase transposons

et al. 1989

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“…3 and ending at 1354 could code for a protein of 194 amino acids with a molecular weight of 21,393. The assignment of this sequence to the tnpR gene is supported by the similarities between the resulting amino acid sequence and the sequences of the resolvase of Tn3 and Tn2J (7,11) (Fig. 4B region between 712 and 748: the suggested -35 sequence (Fig.…”

Section: K R P G R P S a L N E E Q Q L T V I A R I N A G I S I Smentioning

confidence: 75%

Tn2501, a component of the lactose transposon Tn951, is an example of a new category of class II transposable elements

Michiels¹,

Cornelis²,

Ellis³

et al. 1987

J Bacteriol

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Tn2501 is a cryptic class II transposon found as part of the lactose transposon Tn951. Insertional inactivation and nucleotide sequence analysis of Tn2501 allowed us (i) to localize the transposase (tnpA) and the resolvase (tnpR) genes as well as the resolution site (res) of Tn2501 and (ii) to compare Tn2501 with other well-known elements of the two subgroups of class II transposons (Tn3, gamma delta, Tn951, IS101; and Tn21, Tn501, Tn1721). The genetic organization of Tn2501 is similar to that of Tn3 with divergent transcription of the tnpA and tnpR genes away from the intervening res site. The tnpR gene of Tn2501 shows weak homology with that of Tn3 and even less with those of Tn21 and Tn501. However, the tnpA gene and the inverted repeat sequences of Tn2501 present more homology with those of Tn21 and Tn501 than with those of Tn3. Complementation studies showed that TnpA- mutants of Tn2501 can be complemented, at a low frequency, by the Tn21 transposase. None of the tested transposons complemented TnpR- mutants of Tn2501.

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“…This is at position 4764 of the complete Tn501 sequence (N. L. Brown, R. D. Pridmore & D. C. Fritzinger, unpublished data; position 535 in that part of the Tn501 sequence published in Diver et al, 1983). The autoradiograph from the cleavage site-location experiment (Brown & Smith, 1980) using this recombinant DNA is shown in Fig.…”

Section: '-T-c-a-t-t-g-g-a-c-c-g-c-t-g-a-3' 3'-a-g-t-a-a-c-c-t-g-g-cmentioning

confidence: 89%

“…These data were then compared with the computer-generated tables of Fuchs et al (1980) in order to indicate possible recognition sites. Single-stranded recombinant Ml3mp2 and M13mp7 DNA derivatives (Gronenborn & Messing, 1978;Messing et al, 1981) containing a DNA fragment with a single site for the restriction enzyme were obtained from a DNA-sequencing project in this laboratory Diver et al, 1983). The method for identifying the phosphodiester bonds cleaved by the restriction endonuclease is described in detail elsewhere (Brown & Smith, 1980;Whitehead & Brown, 1982).…”

Section: Introductionmentioning

confidence: 99%