DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

McGeoch, Duncan J.; Dolan, Aidan; Frame, Margaret C.

doi:10.1093/nar/14.8.3435

Cited by 63 publications

(39 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed Mr is in reasonable agreement with that predicted: either 51K for the intact protein (McGeoch et al, 1986b), or 46 to 47K if cleavage occurs after the potential signal sequence. The UL10 polypeptide labels poorly with both [3H]mannose and [14C]glucosamine (preliminary observations), and further work is in progress to determine whether it is indeed glycosylated.…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins

MacLean

Efstathiou

Elliott

et al. 1991

Journal of General Virology

111

106

View full text Add to dashboard Cite

The herpes simplex virus type 1 genome contains four open reading frames (ORFs) which are predicted to encode hydrophobic proteins with the potential to cross a membrane several times. The products of these genes (genes UL10, UL20, UL43 and UL53) have not previously been identified. To investigate the role of these proteins in the virus life cycle, we attempted to inactivate the genes individually by inserting the lacZ gene from Escherichia coli within the ORFs. Using this approach we have isolated insertion mutants for UL10 and UL43, as well as a deletion mutant lacking the majority of the UL43 ORF. The growth of the UL10-lacZ virus was slightly impaired in tissue culture compared to that of the wild-type virus parent, whereas the growth of the UL43 mutants was indistinguishable from that of wild-type virus. Furthermore, deletion of the majority of the UL43 ORF did not impair the ability of the virus to replicate in vivo at the periphery, or to spread to and replicate within the nervous system, in a mouse ear model. Repeated attempts to isolate lacZ insertion mutants for UL20 and UL53 were unsuccessful, suggesting that these genes may be essential for virus growth, at least in tissue culture. Using antipeptide sera, the products of genes UL10 and UL20 have been detected.

show abstract

Section: Discussionsupporting

confidence: 86%

“…There are two potential in-frame translation initiation codons for UL10 (McGeoch et al, 1986b). Thus, UL10 may encode a polypeptide of 473 or 455 amino acids, possessing eight potential transmembrane regions in either case and a potential N-terminal signal sequence (McGeoch, 1985) if the second initiation codon is used.…”

Section: Introductionmentioning

confidence: 99%

Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins

MacLean

Efstathiou

Elliott

et al. 1991

Journal of General Virology

111

106

View full text Add to dashboard Cite

show abstract

“…The ULIO protein associates with membranes from infected cells UL10 is predicted to encode a membrane protein (McGeoch et al, 1986). To determine whether the UL10 polypeptide is membrane-associated, cytosol and membrane fractions were isolated from infected ce!ls labelled with [~SS]methionine, and immunoprecipitated with the anti-UL10 serum (see Methods).…”

Section: Resultsmentioning

confidence: 99%

Characterization of the UL10 gene product of herpes simplex virus type 1 and investigation of its role in vivo

MacLean

Robertson

Jamieson

1993

Journal of General Virology

View full text Add to dashboard Cite

(ULlO-del). The in vitro growth properties of this virus were consistent with previous studies; it grew to give slightly lower yields than wild-type and revertant viruses, and had no apparent temperaturesensitive or host range phenotype. In vivo, in a mouse model, ULlO-del was capable of establishing a latent infection, although it was impaired for growth at the periphery, and for spread to and/or growth within the nervous system relative to wild-type or revertant viruses.

show abstract

“…2). The RNAs for the homologous reading frames in HSV-1 [5,29], HCMV [24], and EBV [2] have also been detected with similar sizes of that of MDV2 in late phase of the virus-infected cells.…”

Section: Nucleotide Sequence and Gene Arrangement Of An 1778 Bp Fragmentioning

confidence: 99%

Identification and Structure of the Marek's Disease Virus Serotype 2 Glycoprotein M Gene: Comparison with Glycoprotein M Genes of Herpesviridae Family

Cai

Jang

Izumiya

et al. 1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We determined the nucleotide sequence of a portion of BamHI-C fragment of Marek's disease virus serotype 2 (MDV2) strain HPRS24 which was suspected to contain the homologue of the herpes simplex virus type 1 (HSV-1) gene UL10, encoding glycoprotein M (gM). An open reading frame whose translation product exhibited significant similarities to HSV-1 gM protein and respective proteins of other herpesviruses of 37.5% and 45.5% to 31.8%, respectively, was identified. A number of distinct transcriptional consensus sequences were found upstream of the first putative start codon of MDV2 UL10 protein. In transcriptional analysis, the gene was transcribed into an 1.5 kb RNA. The primary translation product comprises 424 amino acids with a predicted molecular weight of 46.9 kDa. The predicted MDV2 UL10 protein contains eight hydrophobic domains with sufficient length and hydrophobicity to span the lipid bilayer conserved in the genomes of all herpesviruses which have been sequenced so far. In the region located between the first and second hydrophobic domains, two potential N-linked glycosylation sites were presented. Interestingly, highly charged residues were abundantly possessed in the carboxy-terminal part of the MDV2 UL10 protein. By comparison of the amino acid sequence of the MDV2 UL10 gene with the homologues from other herpesviruses, the data might contribute for further evidence of the evolution of herpesviruses from a common progenitor and an ancient example of MDV2 belonging to the Alphaherpesvirinae subfamily. In addition, the existence of corresponding genes in human, mammalian, and avian herpesvirus genomes, suggests indirectly an important role for gM in the natural life cycle of the virus.-KEY WORDS: gM, MDV, MDV2, UL10.J. Vet. Med. Sci. 61(5): 503-511,1999 throughout the Herpesviridae family, including homologues of herpes simplex virus type 1 (HSV-1) gB, gH, gL, and gM, and so far, others, such as gC, gD, gE, gG, gI and gK, are found only in the Alphaherpesvirinae subfamily [39].

show abstract

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

Abstract: ABSTRACT

Cited by 63 publications

References 33 publications

Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins

Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins

Characterization of the UL10 gene product of herpes simplex virus type 1 and investigation of its role in vivo

Identification and Structure of the Marek's Disease Virus Serotype 2 Glycoprotein M Gene: Comparison with Glycoprotein M Genes of Herpesviridae Family

Contact Info

Product

Resources

About