DNA Sequence-Dependent Morphological Evolution of Silver Nanoparticles and Their Optical and Hybridization Properties

Abstract

A systematic investigation of the effects of different DNA sequences on the morphologies of silver nanoparticles (AgNPs) grown from Ag nanocube seeds is reported. The presence of 10-mer oligo-A, -T, and -C directed AgNPs growth from cubic seeds into edge-truncated octahedra of different truncation extents and truncated tetrahedral AgNPs, while AgNPs in the presence of oligo-G remained cubic. The shape and morphological evolution of the nanoparticle growth for each system is investigated using SEM and TEM and correlated with UV-vis absorption kinetic studies. In addition, the roles of oligo-C and oligo-G secondary structures in modulating the morphologies of AgNPs are elucidated, and the morphological evolution for each condition of AgNPs growth is proposed. The shapes were found to be highly dependent on the binding affinity of each of the bases and the DNA secondary structures, favoring the stabilization of the Ag{111} facet. The AgNPs synthesized through this method have morphologies and optical properties that can be varied by using different DNA sequences, while the DNA molecules on these AgNPs are also stable against glutathione. The AgNP functionalization can be realized in a one-step synthesis while retaining the biorecognition ability of the DNA, which allows for programmable assembly.

Interaction of flavones with DNA in vitro: structure–activity relationships

“…[10][11][12][13] Deoxyribonucleic acid (DNA) has long stood as a unique polymer due to its role in biology and biochemistry, 14 contains the genetic instructions for the development and functioning of living organismsis, the target molecule for many drugs, especially antitumor therapies, 15,16 always to be the hot topic of research. [17][18][19] In recent years, promoting a research focus many investigators focus on the interaction between small molecules and DNA, [20][21][22] where characterizing polyphenol-DNA interactions has been integral to understanding the bioactivity of said polyphenol micronutrient DNA ligands and presents opportunities for the design of new and effective drugs against a variety of diseases, 23 but rarely consideration about structure-activity relationship between small molecules was taken. Therefore, comparing the interactions between DNA and different flavones, a polyphenol subclass, can shed light on which aspects of flavones which are required for DNA binding and which parts are expendable.…”

Chiral 432 Helicoid II Nanoparticle Synthesized with Glutathione and Poly(T)20 Nucleotide

“…Compared to cubic seeds grown without any additives introduced to growth solution (Figure S2), nanoparticle synthesized with T20 shows more distinct facet development. According to previous studies using oligonucleotide molecules for morphology control in seed‐mediated growth of achiral nanoparticles, oligonucleotide molecules have been described as to passivate seed surfaces or to kinetically affect the deposition of metal to induce morphological changes …”

Discovery of and Insights into DNA “Codes” for Tunable Morphologies of Metal Nanoparticles

“…A) Circular dichroism (CD) measurements demonstrating the disruption and formation of the i‐motif and the G‐quadruplex during the overgrowth of Ag nanocubes in the presence of C10 and G10, respectively. Adapted with permission . Copyright 2014, American Chemical Society.…”

“…While most studies have been focused on gold‐based systems, it is important to be able to expand the metal NPs to make the above approach more generalizable. DNA‐mediated Ag overgrowth on AgNCs as seeds was the first system to demonstrate the DNA sequence dependent nature of Ag nanoparticle growth . Not surprisingly, the trend of affinity of the different oligonucleotides to Ag was different from that of Au.…”

“…Although the nucleobases are often masked due to their supramolecular assembly, the as‐formed secondary structure may still show potentials to interact with the nanoparticle seed's surface. Thus far, the formation of G‐quadraplex and i‐motif has been observed in growth solutions where the overgrowth of silver nanocubes was studied in the presence of short oligomers of DNA (A10, T10, G10, and C10) (Figure A) . The formation of G‐quadruplex occurs only after having added the silver precursor and reductant, indicating that the Ag + ions played a vital role in structure assembly.…”