DNA Sequence-Based Approach for Classifying the Mold Status of Buildings

Hegarty, Bridget; Pan, Annabelle; Haverinen‐Shaughnessy, Ulla; Shaughnessy, Richard; Peccia, Jordan

doi:10.1021/acs.est.0c03904

Cited by 8 publications

(9 citation statements)

References 36 publications

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“…Risk factors for weakened pelvic floor functions and urinary incontinence were analyzed by logistic regression calculating the value of odds ratio (OR). By applying analysis of similarities (ANOSIM), analysis of molecular variance (AMOVA), linear discriminant analysis effect size (LEfSe), software (version 1.0), and principal coordinate analysis (PCoA) based on the Bray–Curtis dissimilarity metrics, group differences were uncovered ( Excoffier et al, 1992 ; Schmidt et al, 2018 ; Hegarty et al, 2020 ). PCoA was conducted by WGCNA package, stat package, and ggplot2 package in R software, and LEfSe was displayed using the Novomagic, a free online platform for data analysis.…”

Section: Methodsmentioning

confidence: 99%

The relationship between pelvic floor functions and vaginal microbiota in 6–8 weeks postpartum women

Zhang

Lin

et al. 2022

Front. Microbiol.

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The impairment of pelvic floor muscle functions and Lactobacillus-deficient vaginal microbiota is common in postpartum women. However, few studies have explored the correlation between pelvic floor muscle functions and vaginal microbiota. Given this research gap, our study aims to investigate any potential association between these two conditions of postpartum women (6–8 weeks after childbirth). A total of 230 women who required postpartum pelvic floor function examination at Peking University International Hospital from December 2021 to April 2022 were enrolled in this study. The collected questionnaire information included progestational weight, body mass index (BMI), weight gain during pregnancy, neonatal weight, delivery type, multiparity, postpartum time, and urinary incontinence (UI). A total of 187 samples of vaginal secretions were collected, and the vaginal microbiota was detected by 16S rRNA sequence analysis. Finally, 183 samples were analyzed in the trial. All individuals were divided into two groups according to the results of pelvic floor muscle assessment to explore the difference between the incidence of postpartum urinary incontinence and vaginal microbiota. We found that the prevalence of UI was higher in the group with weakened pelvic floor muscles. Vaginal delivery, overweight, age, neonatal weight, and weight gain during pregnancy were all risk factors for postpartum urinary incontinence. The vaginal microbiome was no longer Lactobacillus dominant of most postpartum women (91.8%), while the diversity of microbiota increased. The Lactobacillus-deficient community, commonly labeled as community state type (CST) IV, was sub-divided into four communities. The abundance of vaginal Lactobacillus decreased in the group with compromised pelvic muscle functions, while the species richness and diversity increased significantly. In conclusion, the decreased pelvic floor muscle functions of postpartum women 6–8 weeks after delivery may disrupt the balance of vaginal microbiota, and the restoration of pelvic floor functions may contribute to a healthy and balanced vaginal microbiota.

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Section: Methodsmentioning

confidence: 99%

The relationship between pelvic floor functions and vaginal microbiota in 6–8 weeks postpartum women

Zhang

Lin

et al. 2022

Front. Microbiol.

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“…It should be noted that most of these studies were carried out in mouldy dwellings or dwellings in which inhabitants displayed respiratory problems [ 9 , 67 , 72 , 73 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ]. Data are less numerous on normal buildings with no dampness, no visible mould contamination and no respiratory problems for occupants [ 63 , 66 , 75 , 76 , 81 , 82 , 87 , 88 ]. Only a few studies were performed in other types of buildings, such as hospitals, archives and museums [ 64 , 89 , 90 , 91 , 92 , 93 , 94 , 95 ].…”

Section: Microbial Species Identified On Indoor Building Materialsmentioning

confidence: 99%

“…The DNA analysis methods are based on the isolation and determination of DNA sequences that are specific to certain taxonomic groups. Different genes are used for the diagnoses of species such as the fungal internal transcribed spacer (ITS) [ 67 , 81 , 82 ] and β- tubulin gene by using primers Bt2a [ 86 ]. The main advantage is that the culture of microorganisms and the subsequent bias resulting from media selection is not always required and the analysis can be performed on freshly collected samples.…”

Section: Microbial Species Identified On Indoor Building Materialsmentioning

confidence: 99%

Fungal Contamination of Building Materials and the Aerosolization of Particles and Toxins in Indoor Air and Their Associated Risks to Health: A Review

Hallak

Verdier

Bertron

et al. 2023

Toxins

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It is now well established that biological pollution is a major cause of the degradation of indoor air quality. It has been shown that microbial communities from the outdoors may significantly impact the communities detected indoors. One can reasonably assume that the fungal contamination of the surfaces of building materials and their release into indoor air may also significantly impact indoor air quality. Fungi are well known as common contaminants of the indoor environment with the ability to grow on many types of building materials and to subsequently release biological particles into the indoor air. The aerosolization of allergenic compounds or mycotoxins borne by fungal particles or vehiculated by dust may have a direct impact on the occupant’s health. However, to date, very few studies have investigated such an impact. The present paper reviewed the available data on indoor fungal contamination in different types of buildings with the aim of highlighting the direct connections between the growth on indoor building materials and the degradation of indoor air quality through the aerosolization of mycotoxins. Some studies showed that average airborne fungal spore concentrations were higher in buildings where mould was a contaminant than in normal buildings and that there was a strong association between fungal contamination and health problems for occupants. In addition, the most frequent fungal species on surfaces are also those most commonly identified in indoor air, regardless the geographical location in Europe or the USA. Some fungal species contaminating the indoors may be dangerous for human health as they produce mycotoxins. These contaminants, when aerosolized with fungal particles, can be inhaled and may endanger human health. However, it appears that more work is needed to characterize the direct impact of surface contamination on the airborne fungal particle concentration. In addition, fungal species growing in buildings and their known mycotoxins are different from those contaminating foods. This is why further in situ studies to identify fungal contaminants at the species level and to quantify their average concentration on both surfaces and in the air are needed to be better predict health risks due to mycotoxin aerosolization.

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“…Ongoing research efforts show some promise. In one approach, researchers compare indoor to outdoor samples, but with new sequence-based approaches and analytical tools [6] . Another method showing promise is examining the abundance of targeted groups of fungi, based on a priori hypothesize about their likelihood of growing in damp buildings, in damaged homes compared to dry homes [7].…”

Section: Ongoing Effortsmentioning

confidence: 99%

Measuring microbial growth in damp buildings: Ongoing efforts at an age-old public health problem

Adams

2021

Measuring Microbial Growth in Damp Buildings: Ongoing Efforts at an Age-Old Public Health Problem

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For over 2000 years, building dampness has been considered harmful. Through epidemiological efforts, we have established that damp or moldy building materials are linked to increased risk of respiratory problems. While this is presumably because of unhealthy microbial emissions, the specific causative agent(s) responsible for the health effects remain unknown. In order to identify damp buildings, we want to identify a consistent signature of microbial growth in buildings that can be detected among the rich microbial milieu normally present. This talk will provide a summary of the state of the field (hopefully addressing some long-standing misconceptions) and present findings on promising new strategies, including using sequence-based technologies, for identifying microbial growth in damp buildings. Current research on the moisture conditions in buildings under which microbes are active and contribute to volatile chemistry will also be presented.Peer-review under the responsibility of the organizing committee of the ICMB21.

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DNA Sequence-Based Approach for Classifying the Mold Status of Buildings

Cited by 8 publications

References 36 publications

The relationship between pelvic floor functions and vaginal microbiota in 6–8 weeks postpartum women

The relationship between pelvic floor functions and vaginal microbiota in 6–8 weeks postpartum women

Fungal Contamination of Building Materials and the Aerosolization of Particles and Toxins in Indoor Air and Their Associated Risks to Health: A Review

Measuring microbial growth in damp buildings: Ongoing efforts at an age-old public health problem

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