DNA Sequence and Regional Assignment of the Human Follicle-Stimulating Hormone β-Subunit Gene to the Short Arm of Human Chromosome 11

Abstract

A human genomic DNA fragment in phage lambda containing FSHB, the gene for the beta-subunit of human follicle-stimulating hormone (FSH-beta), was analyzed and the nucleotide sequence of the region of the clone encoding FSH-beta was determined. A subclone of the lambda phage containing 67% of FSH-beta coding sequence was used as hybridization probe to determine the human chromosomal location of FSHB. A panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was screened with the FSHB probe; complete cosegregation of FSHB with human chromosome 11 was observed in all 26 cell hybrids tested. Analysis of a set of cell hybrids containing translocated derivatives of chromosome 11 further localized FSHB to the human chromosome region 11p11.2----11pter. A Hind III restriction fragment length polymorphism (RFLP) detected by another subclone of the lambda phage containing FSHB now provides a genetic marker for this region of the human genome.

Somatic cell hybrid and long-range physical mapping of 11p13 microdissected genomic clones.

“…The map was assembled using six clones (23, 3E, 7E, 61, 20E, and 68E) from the microdissection library, four clones (57, 530, 706, and 1104) previously isolated from the Livermore Laboratory chromosome 11 library (14), and probes for the CAT (35) and FSHB (36) genes. The 7.5-Mb map extends from the centromere proximal side of CAT, through the WAGR region, to the distal side of FSHB.…”

Chromosomal localisation of two putative 11p oncosuppressor genes involved in human ovarian tumours

“…1 IpI5.5 pADJ762 (Barker et al, 1984) EcoRI ( pJl9.4 (Housman et al, 1985) BamHI-EcoRI (2.1) BamHI (21-13) P-FSH I lpl3 pSHc-7 (Watkins et al, 1987) HinclI-SacI (0.5) HindlIl (14-12) DllS16 llpl3 P32-1 (Feder et al, 1985) EcoRI (8.9) MspI (11-7.6+6+4-6+4+3) Catalase llpl3 pINT-800 (Quan et al, 1985) EcoRI-PstI (0.8) (Table II). In the analysis of the tumour specimens all the polymorphic probes used were able to detect loss or significant reduction (at least 50%) in autoradiographic signal for one of the restriction fragments, indicating LOH in the corresponding locus (Figure 1).…”

Molecular structures of glycoprotein hormones and functions of their carbohydrate components

“…The amino acid sequence of follitropin-fl has been determined in six mammals [42,78,[91][92][93][94][95][96][97][98][99][100][101] and thyrotropin-,f in five mammals [31,43,[102][103][104][105][106][107][108][109][110][111][112][113]. Each choriogonadotropin f-subunit sequence and that of equine lutropin possesses a C-terminal extension of 25-30 amino acids containing at least four 0-linked carbohydrate moieties [33,[79][80][81][82][83].…”

“…Those encoding human lutropin and human choriogonadotropin f-subunits were mapped to chromosome 19 [138] where they occurred in a cluster [139], whereas the other subunit genes were each found on different chromosomes. The human follitropin-fi gene was on chromosome 11 [92,140], the human thyrotropin-,f gene on chromosome 1 [141,142], and the human a-subunit gene on chromosome 6 [138,141]. The presence of the C-terminal extension in the f-subunits of both equine choriogonadotropin and equine lutropin, and its absence from non-equine lutropins, suggests that this extension resulted from a relatively recent mutation which is probably unrelated to the evolution of the human lutropin-human choriogonadotropin gene cluster.…”