DNA sequence analysis of a mouse pro alpha 1 (I) procollagen gene: evidence for a mouse B1 element within the gene.

Abstract

In a 3.8-kilobase mouse DNA sequence encoding amino acid sequences for the proal(I) chain of type I procollagen, 14 coding sequences were identified which specify a sequence 95% homologous to amino acid residues 568 to %3 of the bovine al(I) chain. All of these coding sequences were flanked by appropriate splice junctions following the GT/AG rule. These observations suggest, but do not prove, that this proal(I) gene is transcriptionally active. Of the 14 coding sequences, 7 were 54 base pairs in length, whereas the remainder were higher multiples of 54 base pairs. Nonrandom utilization of codons pertained throughout all of the coding sequences showing a preference (56%) for U in the wobble position. Two of the intervening sequences encoded imperfect vestiges of coding sequences which exhibited a codon preference different from that of the proal(I) gene proper and were not flanked by splice junctions. One intervening sequence encoded a member of the mouse Bi family of middle repetitive sequences. It was flanked by 8-base-pair direct repeats and had a truncated A-rich region, suggesting that it may be a mobile element. Within this element were sequences which could function as a RNA polymerase III split promoter.

A long, nontranslatable poly(A) RNA stored in the egg of the sea urchin Strongylocentrotus purpuratus.

“…Pre-mRNAs are also typically polyadenylated, and in organisms whose genomes display short period repeat sequence interspersion, repetitive sequence elements occur frequently within introns (see, e.g., Cochet et al 1979;Wahli et al 1981;Monson et al 1982;Schibler et al 1982;Tsukada et al 1982;Maroteaux et al 1983;Ryffel et al 1983;Suske et al 1983). Thus, one possibility is that the interspersed maternal transcripts are unprocessed oocyte pre-mRNAs that have found their way into the cytoplasm and have been stabilized there, as are stored maternal mRNAs.…”

Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice

“…Briefly, a set of primers was designed for mouse type I procollagen ␣1-chain [␣1(I)] to amplify a 210-bp cDNA fragment (24). To generate competitor cDNA for ␣1(I) competitive PCR assay, the above desired primer sequences were engineered into a heterologous DNA fragment using same strategy as we documented earlier (40).…”

Generation of collagenase-resistant collagen by site-directed mutagenesis of murine pro alpha 1(I) collagen gene.

“…In the Movl3 mouse strain, the Collal gene is inactivated due to a proviral insertion while transcription of the Colla2 gene is not affected (12)(13)(14). Fibroblast cell lines derived from Movl3 homozygous embryos are deficient not only in the production of al(I) chains but also in the production of stable a2(I) collagen chains because triple helices are not formed in the absence of proal(I) chains (15 (16,37).…”