DNA scission inhibition, antioxidant, and antiproliferative activities of water chestnut (Trapa natans) extracted in different solvents

Abstract

This investigation deals with the study of polyphenolic, antioxidant, and antiproliferative activities of water chestnut extracted in different solvents. The antioxidant activities of the extract in different solvents were the maximum in methanol, followed by ethanol and aqueous solution and were found to be 39.8%, 34.1% and 32.6% from DPPH assay, and 45.64%, 35.08% and 30.52% from lipid peroxidation assay, respectively, whereas the reducing power inhibition assay showed the maximum antioxidant potential in the aqueous extract of the sample, followed by methanolic and ethanolic extracts. The polyphenolic content of the water chestnut was found to be more in methanol than in aqueous and ethanolic extracts. The water chestnut extract also showed the antiproliferative potential when tested against the human colon cancer cell line (Colo-205), human ductal breast epithelial tumor cell line (T47D), and human breast adenocarcinoma cell line (MCF7). It also showed some protection from DNA damage induced by hydroxyl radicals in calf thymus DNA.Keywords: water chestnut; DNA scission inhibition; antioxidant activity; antiproliferative activity Esta investigación trata de un estudio sobre la actividad antioxidante, polifenólica y antiproliferativa de la castaña de agua extraída en diferentes solventes. La actividad antioxidante del extracto en diferentes solventes fue máxima en metanol, seguida por etanol y acuoso, además de encontrarse que era de 39,8%; 34,1% y 32,6%; en el ensayo DPPH y 45%; 64%; 35,08% y 30,52% en el ensayo de peroxidación lipídica respectivamente, mientras que el ensayo de reducción del poder de inhibición mostró el máximo potencial antioxidante en el extracto acuoso de la muestra, seguido por los extractos de metanol y etanol. Se encontró que el contenido polifenólico de la castaña de agua era más presente en metanol que en los extractos acuosos y de etanol. El extracto de castaña de agua también mostró el potencial antiproliferativo cuando se analizadó frente a la línea de la célula humana de cáncer de colon (Colo-205), la línea de la célula humana de tumor ductal epitelial de pecho (T47D) y la línea de la célula humana del adenocarcinoma de pecho (MCF7). También mostró cierta protección del daño del ADN inducido por radicales hidroxilos en ADN de timo de ternera.Palabras clave: castaña de agua; escisión inhibitoria del ADN; actividad antioxidante; actividad antiproliferativa

Mushroom varieties found in the Himalayan regions of India: Antioxidant, antimicrobial, and antiproliferative activities

“…Effect of mushroom extracts on cell viability Antioxidants are known to restrain cell proliferation and apoptosis, which depends on the nature of antioxidants, types of tumor cells, and treatment program (43)(44)(45). The present study showed that all the mushroom varieties possess antiproliferative activity against Colo-205 and MCF7, as shown in Fig.…”

Antioxidant and Antiproliferative Activity of Walnut Extract (Juglans regiaL.) Processed by Different Methods and Identification of Compounds Using GC/MS and LC/MS Technique

“…The total phenolic content (TPC) of walnut extracts were determined using the Folin–Ciocalteu reagent method (Gani et al . ) with certain modifications. The sample extract (100 µL) was added to 1.5 mL of freshly prepared Folin–Ciocalteu reagent (1:10 v/v with water).…”

In vitro antioxidant and antiproliferative activity of microwave-extracted green tea and black tea (Camellia sinensis): a comparative study

“…The percent inhibition of black and green tea at a concentration of 20 µg/ml was 9.7% and 10.1% which increased to 48.06% and 56.06% at 100 µg/ml, respectively, which is less than that of BHT (67.76%), the known standard at the same concentration. Gani et al [18] reported that inhibition of lipid peroxidation may be associated with the presence of phenolics in the extract.…”

“…Total phenolic content was determined based on the method described by Gani et al [18] with some Eppendoff) at 37°C in 5% CO 2 with 98% relative humidity. Cells were allowed to adhere and grow for 24 h in culture medium before exposure to extract.…”