DNA restriction and modification systems in Salmonella

Bullas, Leonard R.; Colson, Charles

doi:10.1007/bf00268970

Cited by 27 publications

(2 citation statements)

References 18 publications

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“…DNA hybridization experiments confirmed the similarity between the E. coli K and B systems (Sain and Murray, 1980). The hsd genes for the E. coli K and B systems as well as those for the E. coli A, Salmonella typhimurium SB, and Salmonella potsdam SP systems map close to, and counter-clockwise to, serB (Boyer, 1964;Glover and Colson, 1969;Arber and Wauters-Willems, 1970;Colson and Van Pel, 1974;Bullas and Colson, 1975). Evidence from complementation tests suggests that the hsdSB genes of S. typhimurium and the hsdSP genes of S. potsdam are related to the hsdK genes of E. coli K12 Bullas et al, 1976).…”

Section: Introductionmentioning

confidence: 58%

Structural homologies among type I restriction-modification systems.

Murray¹,

Gough²,

Suri³

et al. 1982

The EMBO Journal

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Structural homologies among different restriction systems of Escherichia coli and several Salmonella species have been investigated by immunological methods using antibodies prepared against two subunits of the E. coli K12 restriction enzyme, and by DNA hybridization experiments using different fragments of the E. coli K12 hsd genes as probes. The results with both techniques show a strong homology between the E. coli K12 and B restriction‐modification systems, weaker but nevertheless marked homology between E. coli K12 and the Salmonella systems SB, SP, and SQ and, surprisingly, no homology between the E. coli K12 and A systems.

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Section: Introductionmentioning

confidence: 58%

Structural homologies among type I restriction-modification systems.

Murray¹,

Gough²,

Suri³

et al. 1982

The EMBO Journal

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show abstract

“…The amino-acid changes resulting from these are displayed in Figure 1B (BrxC) and Figure 1C (PglZ). The multiplicity of changes we attribute to the use of nitrosoguanidine mutagenesis during isolation of the stySA29 allele [39,44]. The R -M + phenotype originally reported for the lineage [40] was unstable, losing modification (M + ) ability [45].…”

Section: Stysa Is a Brex Homologmentioning

confidence: 99%

Reassembling a cannon in the DNA defense arsenal: genetics of StySA, a BREX phage exclusion system in Salmonella lab strains

Zaworski

Dagva

Brandt

et al. 2021

Preprint

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Understanding mechanisms that shape horizontal exchange in prokaryotes is a key problem in biology. A major limit on DNA entry is imposed by restriction-modification (RM) processes that depend on the pattern of DNA modification at host-specified sites. In classical RM, endonucleolytic DNA cleavage follows detection of unprotected sites on entering DNA. Recent investigation has uncovered BREX systems, RM-like activities that employ host protection by DNA modification but replication arrest without evident nuclease action on unmodified phage DNA. We show that the historical stySA RM locus of Salmonella enterica sv Typhimurium is a BREX homolog. The stySA29 allele of the hybrid strain LB5000 carries a mutated version of the ancestral LT2 BREX system. Surprisingly, both a restriction and a methylation defect are observed for this lineage despite lack of mutations in brxX, the modification gene homolog. Instead, flanking genes pglZ and brxC each carry multiple mutations (μ) in C-terminal domains. To avoid plasmid artifacts and potential stoichiometric interference, we chose to investigate this system in situ, replacing the mutated pglZμ and brxCμ genes with wild type (WT). PglZ-WT supports methylation in the presence of either BrxCμ or BrxC-WT but not in the presence of a deletion/insertion allele, ΔbrxC::cat. Restriction of phage L requires both BrxC-WT and PglZ-WT, implicating the BrxC C-terminus specifically in restriction activity. Disruption of four other CDS with cat cassettes still permitted modification, suggesting that BrxC, PglZ and BrxX are principal components of the modification activity. BrxL is required for restriction only. A partial disruption of brxL disrupts transcription globally.

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The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

Suri¹,

Shepherd²,

Bickle³

1984

The EMBO Journal

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been isolated. It proves to be an unusual enzyme, clearly related functionally to the classical type I restriction enzymes. The basic enzyme is a two subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. The DNA recognition sequence of EcoA has an overall structure that is very similar to previously determined type I sequences. It is: 5'-GAGNNNNNNNGTCA-3' 3'-CTCNNNNNNNCAGT-5' where N can be any nucleotide. Modification methylates the adenosyl residue in the specific trinucleotide and the adenosyl residue in the lower strand of the specific tetranucleotide.

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DNA restriction and modification systems in Salmonella

Cited by 27 publications

References 18 publications

Structural homologies among type I restriction-modification systems.

Structural homologies among type I restriction-modification systems.

Reassembling a cannon in the DNA defense arsenal: genetics of StySA, a BREX phage exclusion system in Salmonella lab strains

The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

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