DNA-Responsive Hydrogels That Can Shrink or Swell

Murakami, Yoshihiko; Maeda, Mizuo

doi:10.1021/bm0504330

Cited by 193 publications

(157 citation statements)

References 10 publications

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“…The strategy of the ultrasensitive microchip integrated with smart microgel presented here circumvents the difficulties in simultaneously reducing the detection limit and improving the easy-to-operate property of detection techniques for trace analytes. It can be used to construct versatile new detection platforms for real-time detection of various kinds of trace analyte signals just by incorporating other stimuliresponsive microgels (6,(24)(25)(26)(27)(28), which might be a fertile area of research. Due to the excellent ultrasensitive, fast, and easy-tooperate properties, the detection platforms equipped with microchips incorporating smart microgel will provide ever-better performances in myriad applications including environmental protection, disease diagnosis and epidemic prevention, and may open up new areas of application for hydrogel-based detection techniques.…”

Section: Discussionmentioning

confidence: 99%

Ultrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes

Lin

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Real-time online detection of trace threat analytes is critical for global sustainability, whereas the key challenge is how to efficiently convert and amplify analyte signals into simple readouts. Here we report an ultrasensitive microfluidic platform incorporated with smart microgel for real-time online detection of trace threat analytes. The microgel can swell responding to specific stimulus in flowing solution, resulting in efficient conversion of the stimulus signal into significantly amplified signal of flow-rate change; thus highly sensitive, fast, and selective detection can be achieved. We demonstrate this by incorporating ion-recognizable microgel for detecting trace Pb 2+ , and connecting our platform with pipelines of tap water and wastewater for real-time online Pb 2+ detection to achieve timely pollution warning and terminating. This work provides a generalizable platform for incorporating myriad stimuli-responsive microgels to achieve ever-better performance for real-time online detection of various trace threat molecules, and may expand the scope of applications of detection techniques.

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Section: Discussionmentioning

confidence: 99%

Ultrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes

Lin

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…93 As a result, the gel network was permanently crosslinked unless the DNA chain was cleaved. If the DNA can fold into a hairpin ( Figure 6B), the gel was initially in a more compact state due to the short DNA end-to-end distance.…”

Section: Reversible Change Of Gel Propertymentioning

confidence: 99%

Oligonucleotide-functionalized hydrogels as stimuli responsive materials and biosensors

2011

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Hydrogels are crosslinked hydrophilic polymers that undergo swelling in water. The gel volume is affected by many environmental parameters including temperature, pH, ionic strength, and solvent composition. Therefore, these factors have been traditionally used for making smart hydrogels. DNA, on the other hand, is a special block copolymer. Incorporation of DNA within a hydrogel network can have several important effects. For example, DNA can serve as a reversible crosslinker modulating the mechanical and rheological properties of a hydrogel. Second, DNA can selectively bind to a variety of different molecules. Attaching these binding DNAs inside (aptamers) the hydrogel makes it possible to expand the range of stimuli to chemical and biological molecules. At the same time, the gel matrix can also improve DNA-based sensors and materials. For example, the hydrogel can be dried for storage and rehydrated prior to use and the immobilized DNAs are protected from nuclease cleavage. The gel backbone property can also be tuned to affect the interaction between DNA and other molecules. The rational functionalization of DNA in hydrogels has generated a diverse range of smart materials. In the last 15 years, the field has made tremendous progress and some of the recent developments have been summarized in this review. Challenges and possible future directions are also discussed.

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“…To address this, a number of smart gels with volume change have been reported. Murakami and Maeda employed DNA strands with each of the ends labeled with an amino group to permanently crosslink the gel (but not through hybridization) [64]. The DNA can 8 fold into a hairpin ( Figure 3A), resulting in compact gels due to the short DNA end-to-end distance.…”

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%