DNA Replication in X-Irradiated Chinese Hamster Cells Made Permeable to Deoxyribonucleoside Triphosphates

Olson, Ann C.; Billen, Daniel

doi:10.2307/3574771

Cited by 11 publications

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“…A rapid decrease in DNA synthesis is evident up to 400 rads which gives rise to a more resistant component at higher doses. These results are in keeping with a mode of inhibition of DNA synthesis by ionizing radiation in other mammalian cells (7)(8)(9). Surprisingly in AT cells no inhibition of DNA synthesis is evident up to 400 rads ( Fig.…”

Section: Thesupporting

confidence: 81%

“…Exposure of manmnalian cells to ionizing radiation results in a depression in DNA synthesis (7)(8)(9). A dose response curve is characterized by radioresistant and radiosensitive components of DNA synthesis (7-9).…”

Section: Introductionmentioning

confidence: 99%

“…The radioresistant component appears to reflect the effect of irradiation on the rate of DNA chain growth (9), whereas the more sensitive component is due to an inhibition of initiation of DNA replication (10). An initial depression in the rate of DNA synthesis in cells exposed to moderate doses of ionizing radiation is observed, followed by a recovery of normal rates 3-4 hr postirradiation (7)(8)(9). We have determined the effect of ionizing radiation on DNA synthesis in control and AT homozygote cells as a means of investigating the nature of the defect in AT.…”

Section: Introductionmentioning

confidence: 99%

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Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells

1980

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The effect of ionizing radiation on DNA synthesis in control and ataxia telangiectasia (AT) lymphoblastoid cell lines was determined. A dose dependent decrease in DNA synthesis was observed in control cells, and the rate and extent of thi decrease in synthesis increased with time after irradiation. No decrease in DNA synthesis was obtained in AT cells, immediately following irradiation, at doses up to 400 rads. At longer times postirradiation, inhibition of synthesis increased but the extent of inhibition was less in AT cell than controls at all doses used. An immediate depression of DNA synthesis was evident in control cells after a radiation dose of 200 rads reaching a maximum at 90 min postirradiation. Little or no decrease in DNA synthesis was evident in AT cells up to 60 min after the same radiation dose, but a decrease occurred between 60 and 90 min after irradiation. The rate of recovery of DNA synthesis to normal levels was more rapid in AT cells than in controls.

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Section: Thesupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells

1980

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The effect of bleomycin on DNA synthesis in ataxia telangiectasia lymphoid cells

Cohen

Simpson

1982

Environ. mutagen

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Bleomycin, a radiomimetic glycopeptide, inhibits de novo DNA synthesis in ataxia telangiectasia lymphoblastoid B cells to a markedly lesser extent than in normal and xeroderma pigmentosum lymphoid cells. This observation is similar to that following ionizing radiation; however, the effect is slower following the chemical treatment. Recovery of the normal cells occurs 15-18 hours after treatment, whereas the ataxia telangiectasia lines do not attain normal levels of DNA synthesis during the entire 24-hour observation period. Similar differences were not observed following treatment with mitomycin C, a bifunctional alkylating agent, indicating a specific effect of bleomycin on DNA synthesis in ataxia telangiectasia cells. Following bleomycin treatment and preincubation with hydroxyurea, residual DNA synthesis in ataxia telangiectasia cells was similar to that in both normal and xeroderma pigmentosum lymphoid cells, suggesting that the capacity to repair the induced DNA lesion is present.

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Changes Induced in Cell Membranes Adsorbing Animal Viruses, Bacteriophages, and Colicins

Fuchs

Kohn

1983

Modern Aspects of Electrochemistry

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DNA Replication in X-Irradiated Chinese Hamster Cells Made Permeable to Deoxyribonucleoside Triphosphates

Cited by 11 publications

References 0 publications

Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells

Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells

The effect of bleomycin on DNA synthesis in ataxia telangiectasia lymphoid cells

Changes Induced in Cell Membranes Adsorbing Animal Viruses, Bacteriophages, and Colicins

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