DNA Repair-Responsive Engineered Whole Cell Microbial Sensors for Sensitive and High-Throughput Screening of Genotoxic Impurities
Jie Li,
Haotian Ding,
Yuning Zhao
et al.
Abstract:Genotoxic impurities (GTIs) occurred in drugs, and food
and environment
pose a threat to human health. Accurate and sensitive evaluation of
GTIs is of significance. Ames assay is the existing gold standard
method. However, the pathogenic bacteria model lacks metabolic enzymes
and requires mass GTIs, leading to insufficient safety, accuracy,
and sensitivity. Whole-cell microbial sensors (WCMSs) can use normal
strains to simulate the metabolic environment, achieving safe, sensitive,
and high-throughput detection… Show more
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