DNA repair activation and apoptosis suppression in keratinocytes as a possible mechanism of cytoprotective action of plant polyphenols under conditions of UV-irradiation

Abstract

The work investigated the responses of cultured human keratinocytes to ultraviolet radiation in the C range (UVC) with and without a number of plant polyphenolic compounds. The experimental data obtained in this work indicate a cytoprotective effect of the PPs added immediately after UVC exposure. The cytoprotective activity of plant polyphenolic compounds was reduced in the series: acacetin, silybin, baikalein, leontopodium acid, quercetin, cyanidine chloride, taxifolin, trans-ferulic acid. The effect of UVC irradiation with and without of acacetin on the process of phosphorylation of histones H2AX, the triggering mechanism for the repair of single-stranded DNA damage, was also investigated in keratinocytes. It has been established that, H2AX phosphorylation is activated in response to UVC radiation and acacetin has a significant effect on the kinetics of this process. It is concluded that the PPs can reduce the destructive effect of UVR on the skin cells, activating the process of repairing genetic damage.

The ability of plant polyphenols to reduce the number of UVC-induced single-stranded nuclear DNA breaks in keratinocytes

“…Клеточным ответом на повреждение ДНК является активация механизмов репарации, которые позволяют исправить однонитевые разрывы ДНК, используя в качестве матрицы неповрежденную комплементарную цепь [1]. Однако при достаточно сильном повреждении полной репарации не происходит, следствием чего становится апоптотическая гибель клеток [5]. В предыдущем разделе показано, что РПС достоверно повышают жизнеспособность облученных клеток и снижают их апоптотическую гибель.…”