DNA Recombination: In Vivo and In Vitro Studies

Potter, Huntington; Dressler, Dirk

doi:10.1101/sqb.1979.043.01.106

Cited by 35 publications

(5 citation statements)

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“…B. The same joint molecule isomerized so as to show both junctions in the open planar configuration (258). C. The four resolution outcomes, numbered according to the resolution options realized at the left and the right junctions.…”

Section: Preamblementioning

confidence: 99%

Homologous Recombination—Experimental Systems, Analysis, and Significance

Kuzminov

2011

EcoSal Plus

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Homologous recombination is the most complex of all recombination events that shape genomes and produce material for evolution. Homologous recombination events are exchanges between DNA molecules in the lengthy regions of shared identity, catalyzed by a group of dedicated enzymes. There is a variety of experimental systems in E. coli and Salmonella to detect homologous recombination events of several different kinds. Genetic analysis of homologous recombination reveals three separate phases of this process: pre-synapsis (the early phase), synapsis (homologous strand exchange) and post-synapsis (the late phase). In E. coli, there are at least two independent pathway of the early phase and at least two independent pathways of the late phase. All this complexity is incongruent with the originally ascribed role of homologous recombination as accelerator of genome evolution: there is simply not enough duplication and repetition in enterobacterial genomes for homologous recombination to have a detectable evolutionary role, and therefore not enough selection to maintain such a complexity. At the same time, the mechanisms of homologous recombination are uniquely suited for repair of complex DNA lesions called chromosomal lesions. In fact, the two major classes of chromosomal lesions are recognized and processed by the two individual pathways at the early phase of homologous recombination. It follows, therefore, that homologous recombination events are occasional reflections of the continual recombinational repair, made possible in cases of natural or artificial genome redundancy.

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Section: Preamblementioning

confidence: 99%

Homologous Recombination—Experimental Systems, Analysis, and Significance

Kuzminov

2011

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“…1B. (B) The same joint molecule isomerized to show both junctions in the open planar configuration (498). (C) The four resolution outcomes, numbered according to the resolution options realized at the left and the right junctions.…”

Section: Mechanisms Of Dna Damage and Repairmentioning

confidence: 99%

Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ

Kuzminov

1999

Microbiol Mol Biol Rev

846

579

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SUMMARY Although homologous recombination and DNA repair phenomena in bacteria were initially extensively studied without regard to any relationship between the two, it is now appreciated that DNA repair and homologous recombination are related through DNA replication. In Escherichia coli, two-strand DNA damage, generated mostly during replication on a template DNA containing one-strand damage, is repaired by recombination with a homologous intact duplex, usually the sister chromosome. The two major types of two-strand DNA lesions are channeled into two distinct pathways of recombinational repair: daughter-strand gaps are closed by the RecF pathway, while disintegrated replication forks are reestablished by the RecBCD pathway. The phage λ recombination system is simpler in that its major reaction is to link two double-stranded DNA ends by using overlapping homologous sequences. The remarkable progress in understanding the mechanisms of recombinational repair in E. coli over the last decade is due to the in vitro characterization of the activities of individual recombination proteins. Putting our knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation.

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“…By strand isomerization (15), and branch migration, the asymmetric phase, in which one chromatid has the single heteroduplex, becomes symmetric, with hybrid DNA in 0105-1938/80/0045/0211/$ 02.80 both chromatids. Recombination intermediates that would be expected from either the HOLLIDAY or the MESELSON-RADD~NG model have been visualized by electron microscopy in E. coli (12) and in Saccharomyces (1).…”

Section: Introductionmentioning

confidence: 99%

Recombination in diploid vegetative cells of Saccharomyces cerevisiae

Roman

1980

Carlsberg Res. Commun.

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DNA Recombination: In Vivo and In Vitro Studies

Cited by 35 publications

References 0 publications

Homologous Recombination—Experimental Systems, Analysis, and Significance

Homologous Recombination—Experimental Systems, Analysis, and Significance

Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ

Recombination in diploid vegetative cells of Saccharomyces cerevisiae

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